同: FcγRIII、低親和性Fcγ受容体 lowaffinity Fcγ receptor

FcγRIII
NK細胞の抗体依存性細胞介在性細胞傷害(ADCC)に重要

発現細胞

好中球、NK細胞、マクロファージ

機能

Component of low affinity Fc receptor, FcγRIII, mediates phagocytosis and antibody-dependent cell-mediated cytotoxicity ADCC

PrepTutorEJDIC

certificate of deposit / (また『C.D.』)Civil Defense民間防衛

Wikipedia preview

出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2013/04/14 04:47:10」(JST)

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[Wiki en表示]

CD16 is a low affinity Fc receptor.

It is a cluster of differentiation found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages.^[1] It can be used to isolate populations of these cells by antibodies directed towards CD16, utilising FACS (fluorescent-activated cell sorting) or MACS (magnetic-activated cell sorting).

Function

CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies.

References

^ Janeway, Charles (2001). "Appendix II. CD antigens". Immunobiology (5 ed.). New York: Garland. ISBN 0-8153-3642-X.

External links

CD16 Antigens at the US National Library of Medicine Medical Subject Headings (MeSH)

This article on a gene on chromosome 1 is a stub. You can help Wikipedia by expanding it.

UpToDate Contents

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1. NK細胞欠損症候群：臨床症状および診断 nk cell deficiency syndromes clinical manifestations and diagnosis
2. フェルティ症候群の臨床症状と診断 clinical manifestations and diagnosis of feltys syndrome
3. ナチュラルキラー（NK）細胞大顆粒リンパ球性白血病 natural killer nk cell large granular lymphocyte leukemia
4. 運動以外の好中球機能 neutrophil functions other than movement
5. 肝脾T細胞性リンパ腫の臨床症状、病理学的特徴、および診断 clinical manifestations pathologic features and diagnosis of hepatosplenic t cell lymphoma

English Journal

Monocytes as a diagnostic marker of cardiovascular diseases.

Gratchev A, Sobenin I, Orekhov A, Kzhyshkowska J.SourceDepartment of Dermatology, University Medical Center and Medical Faculty Mannheim, Ruprecht-Karls University of Heidelberg, Mannheim, Germany; Blokhin Cancer Research Center, Russian Academy of Medical Sciences, Russia.
Immunobiology.Immunobiology.2012 May;217(5):476-82. Epub 2012 Jan 13.
Inflammation is an important pathogenic factor of cardiovascular diseases. Inflammatory processes induce the organism systemic changes that are sensed by the cells of innate immune system. These systemic changes include increased concentrations of soluble factors capable of activating monocytes in t
PMID 22325375

Aberrant expression of FcγRIIIA (CD16) contributes to the development of atherosclerosis.

Huang Y, Yin H, Wang J, Liu Q, Wu C, Chen K.AbstractPrevious studies have documented that Fc receptor III A of immunoglobulin G (FcγRIIIA, also named CD16) is involved in the development of coronary heart disease (CHD). However, the mechanism responsible for FcγRIIIA's in contribution to CHD development remains largely unclear. Herein, we investigated the possible role of FcγRIIIA in the development of atherosclerosis. Our results showed that the elevated level of FcγRIIIA on monocytes closely correlated to the adhesive efficiency of human umbilical vein endothelial cells (HUVECs) in vitro. Importantly, we also observed increased population of CD16(+) monocytes and elevated CD16 level on monocytes in ApoE(-/-) mice with characterized atherosclerosis after feeding with high-fat diet for 10weeks. The enhancement of CD16 on monocytes closely correlated to increased content of MMP-9 in aorta and increased inflammatory cytokines in sera. In addition, similar to simvastatin, recombinant human M-CSF represented a robust inhibitory influence on plaque instability and inflammation. Taken together, these data established that FcγRIIIA (CD16)-mediated signaling orchestrated by interaction between monocytes and HUVECs, coupled with inflammatory cytokine stimulation and MMP activation, as a fundamental pathway linked to the development of atherosclerotic formation. Inhibition of FcγRIIIA or its signaling thus might represent a promising approach for the prevention and treatment of CHD.
Gene.Gene.2012 Apr 25;498(1):91-5. Epub 2012 Feb 15.
Previous studies have documented that Fc receptor III A of immunoglobulin G (FcγRIIIA, also named CD16) is involved in the development of coronary heart disease (CHD). However, the mechanism responsible for FcγRIIIA's in contribution to CHD development remains largely unclear. Herein, we investiga
PMID 22366371

Methods to measure the binding of therapeutic monoclonal antibodies to the human Fc receptor FcγRIII (CD16) using real time kinetic analysis and flow cytometry.

Harrison A, Liu Z, Makweche S, Maskell K, Qi H, Hale G.SourceMillipore BioPharma Services, 91 Milton Park, Abingdon, Oxfordshire OX14 4RY, UK.
Journal of pharmaceutical and biomedical analysis.J Pharm Biomed Anal.2012 Apr 7;63:23-8. Epub 2012 Jan 30.
Two different methods have been developed to measure the binding of therapeutic antibodies to the low affinity human Fc receptor FcγRIII (CD16). The first measures binding of antibody to recombinant soluble receptor by surface plasmon resonance and the second uses flow cytometry to measure antibody
PMID 22366323

Japanese Journal

CD16(+) natural killer cells play a limited role against primary dengue virus infection in tamarins.

Yoshida Tomoyuki,Omatsu Tsutomu,Saito Akatsuki,Katakai Yuko,Iwasaki Yuki,Iijima Sayuki,Kurosawa Terue,Hamano Masataka,Nakamura Shinichiro,Takasaki Tomohiko,Yasutomi Yasuhiro,Kurane Ichiro,Akari Hirofumi
Archives of virology 157(2), 363-368, 2012-02
… CD16 is a major molecule expressed on NK cells. … To directly assess the role of natural killer (NK) cells in dengue virus (DENV) infection in vivo, CD16 antibody-treated tamarins were inoculated with a DENV-2 strain. … This resulted in the transient depletion of CD16(+) NK cells, whereas no significant effects on the overall levels or kinetics of plasma viral loads and antiviral antibodies were observed in the treated monkeys when compared to control monkeys. …
NAID 120003874271

4)免疫学的異常による流産原因の解明(シンポジウム1「流産の原因と対策」,第63回日本産科婦人科学会・学術講演会)

中島彰俊
日本産科婦人科學會雜誌 63(12), 2185-2197, 2011-12-01
… Results: First topic is the cytokines expression profiles of CD16^-CD56^<bright> … Second is the direct correlation between extravillous trophoblasts (EVTs) and CD16^-CD56^<bright> …
NAID 110008799037

3)免疫機構から見た流産の病態解明とその対策(シンポジウム1「流産の原因と対策」,第63回日本産科婦人科学会・学術講演会)

福井淳史
日本産科婦人科學會雜誌 63(12), 2167-2184, 2011-12-01
… The main population of uterine NK cell is CD16^-/CD56^<bright> … cytokine producing cells and that of peripheral blood NK cell is CD16^+/CD56^<dim> … We reported that the percentages of CD16^+/CD56^<dim> … NK cells in peripheral blood and the endometrium were significantly higher while that of CD16^-/CD56^<btight> … After treatment with Danazol, endometrial CD16^-/CD56^<bright> …
NAID 110008799036

Related Pictures

★リンクテーブル★

リンク元	「リンパ球」「ナチュラルキラー細胞」「食細胞」「表面抗原分類」「LAK細胞」
拡張検索	「CD161」「CD162」
関連記事	「CD1」「CD」

「リンパ球」

Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
Identifiers
Symbol	FCGR3B
Alt. symbols	FCGR3, FCG3
Entrez	2215
HUGO	3620
OMIM	610665
RefSeq	NM_000570
UniProt	O75015
Other data
Locus	Chr. 1 q23

Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Identifiers
Symbol	FCGR3A
Alt. symbols	FCGR3, FCG3
Entrez	2214
HUGO	3619
OMIM	146740
RefSeq	NM_000569
UniProt	P08637
Other data
Locus	Chr. 1 q23

　　[★]

英: lymphocyte (Z)
同: リンパ細胞、免疫細胞 immunocytes

白血球の一種で、無顆粒球に分類される。
末梢血に白血球が4000-9000 /μl存在するとすれば、リンパ球の存在比は26-46.6 %といわれているので、リンパ球は1040-4194 /μl存在。(IMD)

種類

細胞膜上に発現しているリガンド？ (2007前期生理学授業プリント)

	αβTCR	TCR	Ig	CD3	CD4	CD8	CD16	CD56
T細胞	○			○
Tc細胞		○		○		○
Th細胞		○		○	○
B細胞			○
NK細胞							○	○

成長とリンパ球・好中球数(PED.703)

リンパ球数は生後1ヶ月以降に増加して6ヶ月～1年でピークとなり、以降減少して成人と同程度となる。
これに対して好中球は生下時にピークとなり、以降減少して成人と同程度となる。
生後一ヶ月までは好中球優位であり、1ヶ月～2-6歳まではリンパ球優位となり、以降好中球優位となる。

「ナチュラルキラー細胞」

　　[★]

英: natural killer cell, NK cell
同: NK細胞
関: 単球、自然免疫、LAK細胞、ナチュラルキラー細胞活性、natural killer

概念

自然免疫

リンパ球の5-10%を占めるらしい。

Large granular lymphocytes(LGLs) or NK cells account for 5-10% of periphral blood plymphocytes.(HAR.2024)

大型のアズール好性顆粒を有する(SMB.41)

単球と共に自然免疫に関与
ウイルス感染細胞やガン細胞を傷害する細胞で、T細胞に特異的なマーカをもたずに直接標的細胞を攻撃する (人間の正常構造と機能 VIIA血管・免疫 p.29)

抗体依存性細胞毒性(ADCC)

II型アレルギーにかかわる

分子細胞学的な定義

大顆粒リンパ球の形状(15μm以上の大リンパ球。アズール顆粒を3つ以上有する)
CD56(+), sCD3(-), TCR再構成(-), 胚細胞型
MHC非拘束性のキラー活性を有する

機能

自然免疫

activation signalにより、IFN-γの放出や顆粒(パーフォリン、グランザイム)を放出する(IMM.96)

ADCC

IgGのFc部を結合し、顆粒を放出する(IMM.96)

分子マーカー

CD16：IgGのFc部に対するレセプター
CD56：NCAM-I
NK1.1(NKRP-1 = CD161)

~

CD8：IL-2に反応してNK細胞が増殖
CD57：多くの細胞表面で発現

ケモカイン

リガンド	作用	産生
IL-12	NK細胞活性化	INF-γ
IL-18	Th1分化促進	INF-γ

IL-12,IL-18により活性化され、IFN-γを産生

IL-2により活性化し、細胞障害能が高まる (SPU.65)

受容体

NIR

MHC class Iを認識して攻撃を抑制。MHC class Iが少ないと抑制が外れる(IMM.96)

NKR

「食細胞」

　　[★]

英: phagocyte
同: 貪食細胞

[show details]

貪食細胞 : 約 24,500 件
食細胞 : 約 74,100 件
ファゴサイト : 約 1,660 件

貪食細胞 phagocyte : 約 2,200 件
食細胞 phagocyte : 約 13,000 件
ファゴサイト phagocyte : 34 件

食細胞表面レセプター (SMB.38)

リガンド種	受容体	CD	リガンド	発現
抗体	FcγRI	CD64	IgG3, IgG1, IgG4	マクロファージ
	FcγRII	CD32	IgG3, IgG1, IgG2	多核白血球
	FcγRIII	CD16	IgG1, IgG3
補体	CR1	CD35	C3b
	CR3	CD11b/CD18	iC3b
	CR4	CD11c/CD18	iC3b

「表面抗原分類」

　　[★]

英: cluster of differentiation, CD

細胞の表面抗原を認識する抗体を整理した分類

Bリンパ球 CD10,CD19,CD20

Tリンパ球 CD2,CD3,CD5,CD7

幹細胞 CD34

顆粒球 CD13,CD33

単球 CD14：LPSをリガンドとし、Toll-like receptorと共役して細胞内シグナルを伝達する分子。

巨核球 CD41(GpIIb),CD42(GpIb)

NK細胞：CD16(IgGのFc部に対する受容体)、CD56(NCAM-I)

CD1	CD21	CD41	CD61	CD81
CD2	CD22	CD42	CD62	CD82
CD3	CD23	CD43	CD63	CD83
CD4	CD24	CD44	CD64	CD84
CD5	CD25	CD45	CD65	CD85
CD6	CD26	CD46	CD66	CD86
CD7	CD27	CD47	CD67	CD87
CD8	CD28	CD48	CD68	CD88
CD9	CD29	CD49	CD69	CD89
CD10	CD30	CD50	CD70	CD90
CD11	CD31	CD51	CD71	CD91
CD12	CD32	CD52	CD72	CD92
CD13	CD33	CD53	CD73	CD93
CD14	CD34	CD54	CD74	CD94
CD15	CD35	CD55	CD75	CD95
CD16	CD36	CD56	CD76	CD96
CD17	CD37	CD57	CD77	CD97
CD18	CD38	CD58	CD78	CD98
CD19	CD39	CD59	CD79	CD99
CD20	CD40	CD60	CD80	CD100