English Journal

Virulence of Mycobacterium tuberculosis depends on lipoamide dehydrogenase, a member of three multienzyme complexes.

Venugopal A1, Bryk R, Shi S, Rhee K, Rath P, Schnappinger D, Ehrt S, Nathan C.
Cell host & microbe.Cell Host Microbe.2011 Jan 20;9(1):21-31. doi: 10.1016/j.chom.2010.12.004.
Mycobacterium tuberculosis (Mtb) adapts to persist in a nutritionally limited macrophage compartment. Lipoamide dehydrogenase (Lpd), the third enzyme (E3) in Mtb's pyruvate dehydrogenase complex (PDH), also serves as E1 of peroxynitrite reductase/peroxidase (PNR/P), which helps Mtb resist host-react
PMID 21238944

Characterization of rainbow trout branched-chain alpha-keto acid dehydrogenase complex: inter-domain segments of the E2 component affect the overall activity.

Hakozaki M1, Ono K, Suzuki T, Hata H, Mori T, Kochi H.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology.Comp Biochem Physiol B Biochem Mol Biol.2002 Jun;132(2):433-42.
Branched-chain alpha-keto acid dehydrogenase complex (BCKADH) contains decarboxylase (E1), dihydrolipoyl transacylase (E2), and dihydrolipoyl dehydrogenase (E3) as catalytic components. BCKADH purified from rainbow trout (Oncorhynchus mykiss) liver was comparable with mammalian BCKADH in various enz
PMID 12031470

Autoantibodies of sera from patients with primary biliary cirrhosis recognize the alpha subunit of the decarboxylase component of human branched-chain 2-oxo acid dehydrogenase complex.

Mori T1, Ono K, Hakozaki M, Kasukawa R, Kochi H.
Journal of hepatology.J Hepatol.2001 Jun;34(6):799-804.
BACKGROUND/AIMS: The major antigens for anti-mitochondrial autoantibodies in primary biliary cirrhosis (PBC) are the lipoyl-containing components of 2-oxo acid dehydrogenase complexes. Autoantibodies against the E1alpha subunit of the pyruvate dehydrogenase complex (PDH) also have been found, but th
PMID 11451161

Japanese Journal

小野和夫
山陽学園短期大学紀要 38, 1-10, 2007
… 鎖α-ケト酸脱水素酵素複合体(branched-chain α-keto acid dehydrogenase complex,BCKADH)は、3種類の触媒酵素成分;デカルボキシラーゼ(E1,α2β2)、ジヒドロリポイルトランスアシラーゼ(E2)、リポアミドデヒドロゲナーゼ(E3,α2)、およびE1αのリン酸化/脱リン酸化によりBCKADH活性を調節する2種類の調節酵素成分;BCKADHキナーゼ、BCKADHホスファターゼの計5種類の成分酵素からなる。 …
NAID 110007028374

Binding Properties of the Components of Branched-Chain 2-Oxo Acid Dehydrogenase Complex on ELISA.

Mori Tsutomu,Oshima Yasuyoshi,Kochi Hideo
The Journal of Biochemistry 116(5), 1111-1116, 1994
… Binding characteristics among three catalytic components of rat liver branched-chain 2-oxo acid dehydrogenase complex (BCKADH) were investigated by ELISA. …
NAID 130003532335

Purification, resolution, and reconstitution of rat liver branched-chain .ALPHA.-keto acid dehydrogenase complex.

ONO Kazuo,HAKOZAKI Masatoshi,KIMURA Atsushi,KOCHI Hideo
The Journal of Biochemistry 101(1), 19-27, 1987
… Branched-chain α-keto acid dehydrogenase (BCKADH) was solubilized as an enzyme complex from rat liver mitochondria by sonic treatment. … The BCKADH complex was reconstituted by combining E1, E2, and E3, and the formation of the complex was confirmed by analysis by sucrose density gradient centrifugation. …
NAID 130003530153