Tioguanine
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| Clinical data |
| Trade names |
Lanvis, Tabloid, others |
| AHFS/Drugs.com |
International Drug Names |
| MedlinePlus |
a682099 |
Routes of
administration |
by mouth |
| ATC code |
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| Pharmacokinetic data |
| Bioavailability |
30% (range 14% to 46%) |
| Metabolism |
Intracellular |
| Biological half-life |
80 minutes (range 25-240 minutes) |
| Identifiers |
IUPAC name
- 2-amino-1H-purine-6(7H)-thione
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| CAS Number |
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| PubChem CID |
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| IUPHAR/BPS |
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| DrugBank |
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| ChemSpider |
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| UNII |
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| KEGG |
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| ChEBI |
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| ChEMBL |
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| ECHA InfoCard |
100.005.299 |
| Chemical and physical data |
| Formula |
C5H5N5S |
| Molar mass |
167.193 g/mol |
| 3D model (Jmol) |
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InChI
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InChI=1S/C5H5N5S/c6-5-9-3-2(4(11)10-5)7-1-8-3/h1H,(H4,6,7,8,9,10,11) Y
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Key:WYWHKKSPHMUBEB-UHFFFAOYSA-N Y
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Tioguanine, also known as thioguanine or 6-thioguanine (6-TG) is a medication used to treat acute myeloid leukemia (AML), acute lymphocytic leukemia (ALL), and chronic myeloid leukemia (CML). Long term use is not recommended. It is given by mouth.[1]
Common side effects include bone marrow suppression, liver problems and inflammation of the mouth.[1][2] It is recommended that liver enzymes be checked weekly when on the medication.[1] People with a genetic deficiency in thiopurine S-methyltransferase are at higher risk of side effects.[2] Avoiding pregnancy when on the medication is recommended for both males and females.[1] Tioguanine is in the antimetabolite family of medications.[2] It is a purine analogue of guanine and works by disrupting DNA and RNA.[3]
Tioguanine was between 1949 and 1951.[4][5] It is on the World Health Organization's List of Essential Medicines, the most effective and safe medicines needed in a health system.[6] The wholesale price in the developing world is about 7.07 USD per 40 mg pill as of 2014.[7] In the United Kingdom this amount costs the NHS about 4.14 pounds.[1]
Contents
- 1 Medical uses
- 2 Side effects
- 2.1 Hepatic veno-occlusive disease
- 3 Contraindications
- 4 Interactions
- 5 Pharmacogenetics
- 6 Metabolism and pharmacokinetics
- 7 Mechanism of action
- 8 Chemistry
- 9 Names
- 10 References
Medical uses
- Acute leukemias in both adults and children
- Chronic myelogenous leukemia
- Inflammatory bowel disease, especially ulcerative colitis
- Psoriasis[8]
Side effects
- Leukopenia and neutropenia
- Thrombocytopenia
- Anemia
- Anorexia
- Nausea and vomiting
- Hepatotoxicity: this manifests as:
Hepatic veno-occlusive disease
The major concern that has inhibited the use of thioguanine has been veno-occlusive disease (VOD) and its histological precursor nodular regenerative hyperplasia (NRH). The incidence of NRH with thioguanine was reported as between 33-76%.[9] The risk of ensuing VOD is serious and frequently irreversible so this side effect has been a major concern. However, recent evidence using an animal model for thioguanine-induced NRH/VOD has shown that, contrary to previous assumptions, NRH/VOD is dose dependent and the mechanism for this has been demonstrated.[10] This has been confirmed in human trials, where thioguanine has proven to be safe but efficacious for coeliac disease when used at doses below those commonly prescribed.[11] This has led to a revival of interest in thioguanine because of its higher efficacy and faster action compared to other thiopurines and immunosuppressants such as mycophenylate.[12]
Contraindications
- Pregnancy
- Lactation: The safety warning against breastfeeding may have been a conservative assessment, but research evidence suggests that thiopurines do not enter breastmilk.[13]
Interactions
Cancers that do not respond to treatment with mercaptopurine do not respond to thioguanine. On the other hand, some cases of IBD that are resistant to mercaptopurine (or its pro-drug azathioprine) may be responsive to thioguanine.
Pharmacogenetics
The enzyme thiopurine S-methyltransferase (TPMT) is responsible for the direct inactivation of thioguanine to its methylthioguanine base - this methylation prevents thioguanine from further conversion into active, cytotoxic thioguanine nucleotide (TGN) metabolites.[14][15][16] Certain genetic variations within the TPMT gene can lead to decreased or absent TPMT enzyme activity, and individuals who are homozygous or heterozygous for these types of genetic variations may have increased levels of TGN metabolites and an increased risk of severe bone marrow suppression (myelosuppression) when receiving thioguanine.[14] In many ethnicities, TPMT polymorphisms that result in decreased or absent TPMT activity occur with a frequency of approximately 5%, meaning that about 0.25% of patients are homozygous for these variants.[14][17] However, an assay of TPMT activity in red blood cells or a TPMT genetic test can identify patients with reduced TPMT activity, allowing for the adjustment of thiopurine dose or avoidance of the drug entirely.[14][18] The FDA-approved drug label for thioguanine notes that patients who are TPMT-deficient may be prone to developing myelosuppression and that laboratories offer testing for TPMT deficiency.[19] Indeed, testing for TPMT activity is currently one of the few examples of pharmacogenetics being translated into routine clinical care.[20]
Metabolism and pharmacokinetics
A single oral dose of thioguanine has incomplete metabolism, absorption and high interindividual variability. The bioavailability of thioguanine has an average of 30% (range 14-46%). The maximum concentration in plasma after a single oral dose is attained after 8 hours.
Thioguanine, like other thiopurines, is cytotoxic to white cells; as a result it is immunosuppressive at lower doses and anti-leukemic/anti-neoplastic at higher doses. Thioguanine is incorporated into human bone marrow cells, but like other thiopurines, it is not known to cross the blood-brain barrier. Thioguanine cannot be demonstrated in cerebrospinal fluid, similar to the closely related compound 6-mercaptopurine which also cannot penetrate to the brain.
The plasma half-life of thioguanine is short, due to the rapid uptake into liver and blood cells and conversion to 6-TGN. The median plasma half-life of 80-minutes with a range of 25–240 minutes. Thioguanine is excreted primarily through the kidneys in urine, but mainly as a metabolite, 2-amino-6-methylthiopurine. However, the intra-cellular thio-nucleotide metabolites of thioguanine (6-TGN) have longer half-lives and can therefore be measured after thioguanine is eliminated from the plasma.
Thioguanine is catabolized (broken down) via two pathways.[21] One route is through the deamination by the enzyme guanine deaminase to 6-thioxanthine, which has minimal anti-neoplastic activity, then by oxidation by xanthine oxidase of the thioxanthine to thiouric acid. This metabolic pathway is not dependent on the efficacy of xanthine oxidase, so that the inhibitor of xanthine oxidase, the drug allopurinol, does not block the breakdown of thioguanine, in contrast to its inhibition of the breakdown of the related thiopurine 6-mercaptopurine. The second pathway is the methylation of thioguanine to 2-amino-6-methylthiopurine, which is minimally effective as an anti-neoplastic and significantly less toxic than thioguanine. This pathway also is independent of the enzyme activity of xanthine oxidase.
Mechanism of action
Further information: Azathioprine § Mechanism of action
6-Thioguanine is a thio analogue of the naturally occurring purine base guanine. 6-thioguanine utilises the enzyme hypoxanthine-guanine phosphoribosyltransferase (HGPRTase) to be converted to 6-thioguanosine monophosphate (TGMP). High concentrations of TGMP may accumulate intracellularly and hamper the synthesis of guanine nucleotides via the enzyme Inosine monophosphate dehydrogenase (IMP dehydrogenase).[22]
TGMP is converted by phosphorylation to thioguanosine diphosphate (TGDP) and thioguanosine triphosphate (TGTP). Simultaneously deoxyribosyl analogs are formed, via the enzyme ribonucleotide reductase. The TGMP, TGDP and TGTP are collectively named 6-thioguanine nucleotides (6-TGN). 6-TGN are cytotoxic to cells by: (1) incorporation into DNA during the synthesis phase (S-phase) of the cell; and (2) through inhibition of the GTP-binding protein (G protein) Rac1, which regulates the Rac/Vav pathway.[23] An additional effect may be derived from the incorporation of 6-thioguanine into RNA. This yields a modified RNA strand which cannot be read by the ribosomes.
Chemistry
It is a pale yellow, odorless, crystalline powder.
Names
Tioguanine (INN, BAN), or thioguanine (AAN, USAN).
Thioguanine is administered by mouth (as a tablet - 'Lanvis').
References
- ^ a b c d e British national formulary : BNF 69 (69 ed.). British Medical Association. 2015. p. 588, 592. ISBN 9780857111562.
- ^ a b c "Tioguanine 40 mg Tablets - Summary of Product Characteristics (SPC) - (eMC)". www.medicines.org.uk. Retrieved 21 December 2016.
- ^ Golan, David E.; Tashjian, Armen H.; Armstrong, Ehrin J. (2011). Principles of Pharmacology: The Pathophysiologic Basis of Drug Therapy. Lippincott Williams & Wilkins. p. 686. ISBN 9781608312702.
- ^ Sigel, Astrid; Sigel, Helmut (1996). Metal Ions in Biological Systems: Volume 32: Interactions of Metal Ions with Nucleotides: Nucleic Acids, and Their Constituents. CRC Press. p. 302. ISBN 9780824795498.
- ^ Landau, Ralph; Achilladelis, Basil; Scriabine, Alexander (1999). Pharmaceutical Innovation: Revolutionizing Human Health. Chemical Heritage Foundation. p. 342. ISBN 9780941901215.
- ^ "WHO Model List of Essential Medicines (19th List)" (PDF). World Health Organization. April 2015. Retrieved 8 December 2016.
- ^ "Tioguanine". International Drug Price Indicator Guide. Retrieved 28 November 2015.
- ^ Mason C, Krueger GG (January 2001). "Thioguanine for refractory psoriasis: a 4-year experience". J. Am. Acad. Dermatol. 44 (1): 67–72. doi:10.1067/mjd.2001.109296. PMID 11148479.
- ^ Dubinsky MC, Vasiliauskas EA, Singh H, et al. (2003). "6-thioguanine can cause serious liver injury in inflammatory bowel disease patients.". Gastroenterology. 125 (2): 298–303. doi:10.1016/S0016-5085(03)00938-7. PMID 12891528.
- ^ Oancea I, Png CW, Das I, Lourie R, Winkler IG, et al. (July 2012). "A novel mouse model of veno-occlusive disease provides strategies to prevent thioguanine-induced hepatic toxicity.". Gut. 62 (4): 594–605. doi:10.1136/gutjnl-2012-302274. PMID 22773547.
- ^ Tack GJ, van Asseldonk DP, van Wanrooij RL, et al. (August 2012). "Tioguanine in the treatment of refractory coeliac disease – a single centre experience.". Aliment Pharmacol Ther. 36 (3): 274–81. doi:10.1111/j.1365-2036.2012.05154.x. PMID 22646133.
- ^ Van Asseldonk DP, Oancea I, Jharap B, et al. (March 2012). "Is thioguanine-associated sinusoidal obstruction syndrome avoidable? Lessons learned from 6-thioguanine treatment of inflammatory bowel disease and a mouse model.". Rev Assoc Med Bras. 58 (Suppl.1): S8–13.
- ^ Gardiner SJ, Gearry RB, Roberts RL, et al. (2006). "Exposure to thiopurine drugs through breast milk is low based on metabolite concentrations in mother-infant pairs.". Br J Clin Pharmacol. 62 (4): 453–6. doi:10.1111/j.1365-2125.2006.02639.x. PMC 1885151. PMID 16995866.
- ^ a b c d Relling MV, Gardner EE, Sandborn WJ, Schmiegelow K, Pui CH, Yee SW, Stein CM, Carrillo M, Evans WE, Klein TE; Clinical Pharmacogenetics Implementation Consortium (March 2011). "Clinical Pharmacogenetics Implementation Consortium guidelines for thiopurine methyltransferase genotype and thiopurine dosing". Clin Pharmacol Ther. 89 (3): 387–91. doi:10.1038/clpt.2010.320. PMC 3098761. PMID 21270794.
- ^ Zaza G, Cheok M, Krynetskaia N, Thorn C, Stocco G, Hebert JM, McLeod H, Weinshilboum RM, Relling MV, Evans WE, Klein TE, Altman RB (September 2010). "Thiopurine pathway". Pharmcogenet Genomics. 20 (9): 573–4. doi:10.1097/FPC.0b013e328334338f. PMC 3098750. PMID 19952870.
- ^ Fujita K, Sasaki Y (August 2007). "Pharmacogenomics in drug-metabolizing enzymes catalyzing anticancer drugs for personalized cancer chemotherapy". Curr. Drug Metab. 8 (6): 554–62. doi:10.2174/138920007781368890. PMID 17691917.
- ^ Mutschler, Ernst; Schäfer-Korting, Monika (2001). Arzneimittelwirkungen (in German) (8 ed.). Stuttgart: Wissenschaftliche Verlagsgesellschaft. pp. 107, 936. ISBN 3-8047-1763-2.
- ^ Payne, K.; Newman, W.; Fargher, E.; Tricker, K.; Bruce, I. N.; Ollier, W. E. R. (2007). "TPMT testing in rheumatology: Any better than routine monitoring?". Rheumatology. 46 (5): 727–729. doi:10.1093/rheumatology/kel427. PMID 17255139.
- ^ "TABLOID- thioguanine tablet". http://dailymed.nlm.nih.gov/dailymed/drugInfo.cfm?setid=4490128b-e73f-4849-9d6e-e8591639d771.
- ^ Wang L, Pelleymounter L, Weinshilboum R, Johnson JA, Hebert JM, Altman RB, Klein TE (June 2010). "Very important pharmacogene summary: thiopurine S-methyltransferase". Pharmacogenet Genomics. 20 (6): 401–5. doi:10.1097/FPC.0b013e3283352860. PMC 3086840. PMID 20154640.
- ^ Oncea I; Duley J. (2008). "Chapter 38. Pharmacogenetics of Thiopurines.". In Brunton, L. L.; Lazo, J. S.; Parker, K. Goodman & Gilman's The Pharmacological Basis of Therapeutics (11th ed.). McGraw-Hill's Access Medicine (on-line).
- ^ Evans WE. (2004). "Pharmacogenetics of thiopurine S-methyltransferase and thiopurine therapy.". Ther Drug Monit. 26 (2): 186–91. doi:10.1097/00007691-200404000-00018. PMID 15228163.
- ^ de Boer NK, van Bodegraven AA, Jharap B, et al. (Dec 2007). "Drug Insight: pharmacology and toxicity of thiopurine therapy in patients with IBD.". Nat Clin Pract Gastroenterol Hepatol. 4 (12): 686–94. doi:10.1038/ncpgasthep1000. PMID 18043678.
