- 同
- 2D-PAGE
- 同
- 2D-PAGE
WordNet
- indicate the dimensions on; "These techniques permit us to dimension the human heart"
- shape or form to required dimensions
- the magnitude of something in a particular direction (especially length or width or height)
- one of three Cartesian coordinates that determine a position in space
- being one more than one; "he received two messages" (同)2, ii
- the cardinal number that is the sum of one and one or a numeral representing this number (同)2, II, deuce
- of or relating to dimensions
- having dimension--the quality or character or stature proper to a person; "never matures as a dimensional character; he is pasty, bland, faceless"- Norman Cousins
- the motion of charged particles in a colloid under the influence of an electric field; particles with a positive charge go to the cathode and negative to the anode (同)cataphoresis, dielectrolysis, ionophoresis
- a colloid in a more solid form than a sol (同)colloidal gel
- become a gel; "The solid, when heated, gelled"
PrepTutorEJDIC
- 〈C〉(長さ・幅・厚さなどの)『寸法』 / 《通例複数形で》大きさ,範囲,規模 / 〈C〉広がり,展望(scope) / 〈C〉(数学で)次元
- 〈C〉(数の)『2』,2の記号 / 〈U〉2時,2分;2歳 / 《複数扱い》二人;2個 / 〈C〉二つ(二人,2個)1組のもの / 〈C〉(カードゲーム)2の札,(さいころの)2の目 / 2の;二人の;二つの,2個の / 《補語にのみ用いて》2歳の(で)
- (長さ・幅・厚さなどの)寸法の / (数学で)…次元の
- ゲル(コロイド溶液の凝固した状態;ゼラチン・寒天など) / =jell
UpToDate Contents
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- 1. 臨床腫瘍学における遺伝子発現プロファイリング、プロテオミクス、およびマイクロRNAプロファイリングの概要 overview of gene expression profiling proteomics and microrna profiling in clinical oncology
- 2. モノクローナル蛋白の認識 recognition of monoclonal proteins
- 3. 異常ヘモグロビン症の検査室診断 laboratory diagnosis of the hemoglobinopathies
- 4. 肝線維症の非侵襲的評価：超音波エラストグラフィ noninvasive assessment of hepatic fibrosis ultrasound based elastography
- 5. 産婦人科における超音波検査 ultrasound examination in obstetrics and gynecology
English Journal
- Rheological and mechanical behavior of polyacrylamide hydrogels chemically crosslinked with allyl agarose for two-dimensional gel electrophoresis.
- Suriano R1, Griffini G2, Chiari M3, Levi M2, Turri S2.Author information 1Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milan, Italy. Electronic address: raffaella.suriano@polimi.it.2Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milan, Italy.3Istituto di Chimica del Riconoscimento Molecolare, C.N.R., Via Mario Bianco 9, 20131 Milan, Italy.AbstractTwo-dimensional (2-D) gel electrophoresis currently represents one of the most standard techniques for protein separation. In addition to the most commonly employed polyacrylamide crosslinked hydrogels, acrylamide-agarose copolymers have been proposed as promising systems for separation matrices in 2-D electrophoresis, because of the good resolution of both high and low molecular mass proteins made possible by careful control and optimization of the hydrogel pore structure. As a matter of fact, a thorough understanding of the nature of the hydrogel pore structure as well as of the parameters by which it is influenced is crucial for the design of hydrogel systems with optimal sieving properties. In this work, a series of acrylamide-based hydrogels covalently crosslinked with different concentrations of allyl agarose (0.2-1%) is prepared and characterized by creep-recovery measurements, dynamic rheology and tensile tests, in the attempt to gain a clearer understanding of structure-property relationships in crosslinked polyacrylamide-based hydrogels. The rheological and mechanical properties of crosslinked acrylamide-agarose hydrogels are found to be greatly affected by crosslinker concentration. Dynamic rheological tests show that hydrogels with a percentage of allyl agarose between 0.2% and 0.6% have a low density of elastically effective crosslinks, explaining the good separation of high molecular mass proteins in 2-D gel electrophoresis. Over the same range of crosslinker concentration, creep-recovery measurements reveal the presence of non-permanent crosslinks in the hydrogel network that justifies the good resolution of low molecular mass proteins as well. In tensile tests, the hydrogel crosslinked with 0.4% of allyl agarose exhibits the best results in terms of mechanical strength and toughness. Our results show how the control of the viscoelastic and the mechanical properties of these materials allow the design of mechanically stable hydrogels with improved sieving ability in protein electrophoresis over a wide range of molecular masses.
- Journal of the mechanical behavior of biomedical materials.J Mech Behav Biomed Mater.2014 Feb;30:339-46. doi: 10.1016/j.jmbbm.2013.12.006. Epub 2013 Dec 11.
- Two-dimensional (2-D) gel electrophoresis currently represents one of the most standard techniques for protein separation. In addition to the most commonly employed polyacrylamide crosslinked hydrogels, acrylamide-agarose copolymers have been proposed as promising systems for separation matrices in
- PMID 24368174
- Proteome analysis for antifungal effects of Bacillus subtilis KB-1122 on Magnaporthe grisea P131.
- Zhang C, Zhang X, Shen S.Author information Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, 125100, Liaoning, People's Republic of China, cxzhang-bj@163.com.AbstractRice blast, caused by Magnaporthe grisea threatens rice production worldwide. It is important to develop novel and environment-safe strategies to control the fungus. Here we reported that Bacillus subtilis KB-1122 could strikingly inhibit the growth of M. grisea P131 in agar diffusion assays. To further understand the molecular mechanism on the suppressive role of B. subtilis on M. grisea, the antagonist-pathogen interaction of the two strains was studied by using comparative proteome analysis in this report. The cellular and culture supernatant (CSN) proteins were prepared from co-culture and subjected to two-dimensional polyacrylamide gel electrophoresis. Proteome analysis revealed 33 cellular and 18 CSN proteins showing changes upon co-culture respectively. Importantly, down-regulated cellular proteins came from M. grisea, whereas up-regulated proteins derived from B. subtilis. Results suggested that glyceraldehyde-3-phosphate dehydrogenase and serine protein kinase might contribute to antifungal activity of B. subtilis KB-1122. Of CSN proteins identified, the endo-1,4-beta-glucanase (involved in degradation of polysaccharides) was up-regulated consistently at different times of incubation. This suggests that this enzyme plays an important role in the interaction between B. subtilis KB-1122 with M. grisea P131.
- World journal of microbiology & biotechnology.World J Microbiol Biotechnol.2014 Jan 18. [Epub ahead of print]
- Rice blast, caused by Magnaporthe grisea threatens rice production worldwide. It is important to develop novel and environment-safe strategies to control the fungus. Here we reported that Bacillus subtilis KB-1122 could strikingly inhibit the growth of M. grisea P131 in agar diffusion assays. To fur
- PMID 24442786
- Novel regulatory roles of omega-3 fatty acids in metabolic pathways: a proteomics approach.
- Ahmed AA, Balogun KA, Bykova NV, Cheema SK.Author information Department of Biochemistry, Memorial University of Newfoundland, St, John's, NL, A1B 3X9, Canada. skaur@mun.ca.AbstractBACKGROUND: Omega-3 polyunsaturated fatty acids (n-3 PUFA) have been shown to alleviate the symptoms of metabolic disorders, such as heart disease, diabetes, obesity and insulin resistance. Several putative mechanisms by which n-3 PUFA elicit beneficial health effects have been proposed; however, there is still a shortage of knowledge on the proteins and pathways that are regulated by n-3 PUFA.
- Nutrition & metabolism.Nutr Metab (Lond).2014 Jan 17;11(1):6. doi: 10.1186/1743-7075-11-6.
- BACKGROUND: Omega-3 polyunsaturated fatty acids (n-3 PUFA) have been shown to alleviate the symptoms of metabolic disorders, such as heart disease, diabetes, obesity and insulin resistance. Several putative mechanisms by which n-3 PUFA elicit beneficial health effects have been proposed; however, th
- PMID 24438320
Japanese Journal
- Identification of two phosphorylated species of β-catenin involved in the ubiquitin-proteasome pathway by using two-dimensional Phos-tag affinity electrophoresis
- Kinoshita-Kikuta Emiko,Kinoshita Eiji,Koike Tohru
- Journal of Electrophoresis
- … We recently reported a neutral-pH gel system buffered with 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol hydrochloride (Bis-Tris-HCl) for use in Zn2+-Phos-tag sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for advanced profiling of protein phosphorylation. …
- NAID 130004713130
- Alteration in the Expression of Proteins in Unexplained Recurrent Pregnancy Loss Compared with in the Normal Placenta
- GHARESI-FARD Behrouz,ZOLGHADRI Jaleh,KAMALI-SARVESTANI Eskandar
- Journal of Reproduction and Development 60(4), 261-267, 2014
- … Total placental proteins were extracted, and the two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) technique was used for separation of the placental proteomes. … Protein spots differentially expressed between URPL and normal placentas were selected and identified by the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF/TOF) technique after being digested in the gel. …
- NAID 130003390832
- Investigation of Proteomic Profiles of Lamina of Ecklonia kurome (Laminariales): Homology-Based Cross-Species Protein Identification and Analysis of the Post-translational Processing of Vanadium-Dependent Bromoperoxidases Using MALDI-TOF/TOF
- Nagai Kouhei,Morimoto Koichi,Ikegami Haruka,Kimura Hajime,Yotsukura Norishige
- Marine Biotechnology 15(4), 487-498, 2013-08
- … Proteomic profiles of the lamina of Ecklonia kurome Okamura, one of the Japanese dominant laminarialean kelps, were investigated by two-dimensional electrophoresis (2-DE) and MALDI-TOF/TOF. … Proteins were extracted from the lamina by an ethanol/phenol method and subjected to 2-DE (pI 4-7, 10 % polyacrylamide gel). …
- NAID 120005460240
Related Links
- Two-dimensional PolyAcrylamide Gel Electrophoresis (2D PAGE) Click here to see what it is all about. What is 2D Gel Electrophoresis? A method for the separation of proteins in 2 dimensions. 1st Dimension 2nd Dimension ...
- Two-dimensional polyacrylamide gel electrophoresis (2-DE), when combined with mass spectrometry (MS) has the power to identify and quantify differential protein expression of large sample sets analyse ... Abdolzade-Bavil, A ...
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★リンクテーブル★
| リンク元 | 「two-dimensional gel electrophoresis」「二次元ポリアクリルアミドゲル電気泳動」 |
| 関連記事 | 「electrophoresis」「dimension」「dimensional」「polyacrylamide gel」「g」 |
「two-dimensional gel electrophoresis」
「二次元ポリアクリルアミドゲル電気泳動」
「electrophoresis」
「dimension」
「dimensional」
- 次元の
「polyacrylamide gel」
「g」
- 関
- gram