Wikipedia preview

出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2016/01/15 22:44:40」(JST)

wiki en

A spectrofluorometer is an instrument which takes advantage of fluorescent properties of some compounds in order to provide information regarding their concentration and chemical environment in a sample. A certain excitation wavelength is selected, and the emission is observed either at a single wavelength, or a scan is performed to record the intensity versus wavelength, also called an emission spectra. See Fluorescence spectroscopy

Operation

Generally, spectrofluorometers use high intensity light sources to bombard a sample with as many photons as possible. This allows for the maximum number of molecules to be in an excited state at any one point in time. The light is either passed through a filter, selecting a fixed wavelength, or a monochromator, which allows a wavelength of interest to be selected for use as the exciting light. The emission is collected at 90 degrees to the exciting light. The emission is also either passed through a filter or a monochromator before being detected by a photomultiplier tube, photodiode, or charge-coupled device detector. The signal can either be processed as digital or analog output.

Systems vary greatly and a number of considerations affect the choice. The first is the signal-to-noise ratio. There are many ways to look at the signal to noise of a given system but the accepted standard is by using water Raman. Sensitivity or detection limit is another specification to be considered, that is how little light can be measured. The standard would be fluorescein in NaOH, typical values for a high end instrument are in the femtomolar range.

Auxiliary components

These systems come with many options, including:

Polarizers
Peltier temperature controllers
Cryostats
Cold Finger Dewars
Pulsed lasers for lifetime measurements
LEDs for lifetimes
Filter holders
Adjustable optics (very important)
Solid sample holders
Slide holders
Integrating spheres
Near-infrared detectors
Bilateral slits
Manual slits
Computer controlled slits
Fast switching monochromators
Filter wheels

English Journal

Design and synthesis of novel pyrrolo[2,3-a]carbazoles: 7-Chloro-2-oxo-3a-(2'-oxo-2',3'-dihydro-1'H-indol-3'-yl)-2,3,3a,4,5,10-hexahydro-pyrrolo[3,2-a]carbazole-1-carbonitrile as an efficient anticancer agent.

Sathiyachandran P1, Manogaran P2, Nesterov VN3, Padma VV2, Rajendra Prasad KJ4.
European journal of medicinal chemistry.Eur J Med Chem.2018 Apr 25;150:851-863. doi: 10.1016/j.ejmech.2018.03.056. Epub 2018 Mar 21.
PMID 29597168

Fluorescent Polymersomes with Aggregation-Induced Emission.

Zhang N1, Chen H2, Fan Y2, Zhou L1, Trépout S3, Guo J1, Li MH1,2.
ACS nano.ACS Nano.2018 Apr 9. doi: 10.1021/acsnano.8b01755. [Epub ahead of print]
PMID 29617555

SRSF7 knockdown promotes apoptosis of colon and lung cancer cells.

Fu Y1, Wang Y1.
Oncology letters.Oncol Lett.2018 Apr;15(4):5545-5552. doi: 10.3892/ol.2018.8072. Epub 2018 Feb 16.
PMID 29556298

Japanese Journal

Fluorescent quantitation method for differentiating the nativity of green fluorescent protein(METHODS)

Chew Few Ne,Tan Wen Siang,Tey Beng Ti
Journal of bioscience and bioengineering 111(2), 246-248, 2011-02
A gel imaging method was employed to quantitate the GFP that had been subjected to denaturation and degradation treatments. This method is able to differentiate the nativity of GFP by relating the obs …
NAID 110008460342

A05 超音波刺激応答型リポソームを用いたドラッグデリバリーシステムの構築(口頭発表)

田下裕之,仁宮一章,清水宣明
ソノケミストリー討論会講演要旨集 (19), 9-10, 2010-10-21
… After that, the release of calcein from liposome was measured by the fluorescence intensity with a spectrofluorometer. …
NAID 110009710443

Development of a Simple and Low-cost Device for Fluorometric Determination of Selenium in Water Samples

SUZUKI Yasutada,HASHIGAYA Naoki,KAWAKUBO Susumu
Analytical Sciences 26(6), 719-722, 2010
… A portable spectrofluorometer device comprising an ultraviolet LED (380 nm) as a light source, an LED driver, a microsyringe as a cell, an optical fiber cable, a CCD spectrometer and a personal computer was used on-site. … Using fluorescein solution as a standard, the performance of this device was compared with that of a bench-top spectrofluorometer. … The determination was carried out with both the portable device and the spectrofluorometer. …
NAID 130004826976