WordNet
- a way of doing something, especially a systematic way; implies an orderly logical arrangement (usually in steps)
- a thin coating of metal deposited on a surface (同)metal_plating
- the application of a thin coat of metal (as by electrolysis)
- copy that is not the original; something that has been copied (同)replication, reproduction
PrepTutorEJDIC
- 〈C〉(特に秩序だった)(…の)『方法』,方式《+『of』+『名』(do『ing』)》 / 〈C〉〈U〉(思考行・行為・行動の)きちょうめんさ;秩序,筋道
- (金・銀などの)めっき / (船・乗物などの鋼鉄板の)被復,装甲
- (原作者が作った)原作の写し / 模写,複製(copy),模型
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- 1. 胸骨創傷合併症の外科的マネージメント surgical management of sternal wound complications
- 2. 心臓手術後の縦隔炎 postoperative mediastinitis after cardiac surgery
- 3. 先天性漏斗胸：治療 pectus excavatum treatment
- 4. 陰圧創傷閉鎖法 negative pressure wound therapy
- 5. 在胎月齢の生後評価 postnatal assessment of gestational age
English Journal
- Non-invasive in situ monitoring and quantification of TOL plasmid segregational loss within Pseudomonas putida biofilms.
- Ma H, Katzenmeyer KN, Bryers JD.Author information Department of Bioengineering, University of Washington, Seattle, Washington, 98195.AbstractMethods for the detection of plasmid loss in natural environments have typically relied on replica plating, selective markers and PCR. However, these traditional methods have the limitations of low sensitivity, underestimation of specific cell populations, and lack of insightful data for non-homogeneous environments. We have developed a non-invasive microscopic analytical method to quantify local plasmid segregational loss from a bacterial population within a developing biofilm. The probability of plasmid segregational loss in planktonic and biofilm cultures of Pseudomonas putida carrying the TOL plasmid (pWWO::gfpmut3b) was determined directly in situ, in the absence of any applied selection pressure. Compared to suspended liquid culture, we report that the biofilm mode of growth enhances plasmid segregational loss. Results based on a biofilm-averaged analysis reveal that the probability of plasmid loss in biofilm cultures (0.016 ± 0.004) was significantly greater than that determined in planktonic cultures (0.0052 ± 0.0011). Non-invasive assessments showed that probabilities of plasmid segregational loss at different locations in a biofilm increased dramatically from 0.1% at the substratum surface to 8% at outside layers of biofilm. Results suggest that higher nutrient concentrations and subsequentially higher growth rates resulted in higher probability of plasmid segregational loss at the outer layers of the biofilm.
- Biotechnology and bioengineering.Biotechnol Bioeng.2013 Nov;110(11):2949-58. doi: 10.1002/bit.24953. Epub 2013 May 23.
- Methods for the detection of plasmid loss in natural environments have typically relied on replica plating, selective markers and PCR. However, these traditional methods have the limitations of low sensitivity, underestimation of specific cell populations, and lack of insightful data for non-homogen
- PMID 23633286
- Diverse gene cassettes in class 1 integrons of facultative oligotrophic bacteria of River Mahananda,West Bengal, India.
- Chakraborty R, Kumar A, Bhowal SS, Mandal AK, Tiwary BK, Mukherjee S.Author information Department of Biotechnology, Omics Laboratory, University of North Bengal, Siliguri, West Bengal, India. rcnbu2003@yahoo.comAbstractBACKGROUND: In this study a large random collection (n=2188) of facultative oligotrophic bacteria, from 90 water samples gathered in three consecutive years (2007-2009) from three different sampling sites of River Mahananda in Siliguri, West Bengal, India, were investigated for the presence of class 1 integrons and sequences of the amplification products.
- PloS one.PLoS One.2013 Aug 9;8(8):e71753. doi: 10.1371/journal.pone.0071753. eCollection 2013.
- BACKGROUND: In this study a large random collection (n=2188) of facultative oligotrophic bacteria, from 90 water samples gathered in three consecutive years (2007-2009) from three different sampling sites of River Mahananda in Siliguri, West Bengal, India, were investigated for the presence of class
- PMID 23951238
- Xer-cise in Helicobacter pylori: one-step transformation for the construction of markerless gene deletions.
- Debowski AW, Gauntlett JC, Li H, Liao T, Sehnal M, Nilsson HO, Marshall BJ, Benghezal M.Author information Ondek Pty Ltd. and Helicobacter pylori Research Laboratory, Marshall Centre for Infectious Disease Research and Training, School of Pathology & Laboratory Medicine, Discipline of Microbiology & Immunology, The University of Western Australia, M504, L Block, QEII Medical Centre, Nedlands, WA, 6009, Australia.AbstractBACKGROUND: Xer-cise is an efficient selectable marker removal technique that was first applied in Bacillus subtilis and Escherichia coli for the construction of markerless gene deletions. Xer-cise marker excision takes advantage of the presence of site-specific Xer recombination in most bacterial species for the resolution of chromosome dimers at the dif site during replication. The identification and functional characterization of the difH/XerH recombination system enabled the development of Xer-cise in Helicobacter pylori.
- Helicobacter.Helicobacter.2012 Dec;17(6):435-43. doi: 10.1111/j.1523-5378.2012.00969.x. Epub 2012 Jun 24.
- BACKGROUND: Xer-cise is an efficient selectable marker removal technique that was first applied in Bacillus subtilis and Escherichia coli for the construction of markerless gene deletions. Xer-cise marker excision takes advantage of the presence of site-specific Xer recombination in most bacterial s
- PMID 23066820
Japanese Journal
- Micromolding for three-dimensional metal microstructures using stereolithography of photopolymerized resin
- Mukai Kohki,Kitayama Shinya,Kawajiri ?Yasunobu,Maruo Shoji
- Microelectronic engineering. 86(4-6), 1169-1172, 2009
- … We have proposed a metal micromolding method based on the stereolithography of photopolymerized resin. … In this work, we report our recent successful development of three fundamental processes used in the micromolding method. … The electroless plating of palladium with a purity of 93 - 97% was realized on photopolymerized resin with a view to realizing a shape memory alloy. … A copper replica was successfully extracted from a resin mold by using heat treatment. …
- NAID 120004680479
- Fabrication of Stainless Steel Mold Using Electrochemical Fabrication Method for Microfluidic Biochip
- Cho Min-Soo,Lim Hyun-Woo,Lee Caroline Sunyong [他],Cho Byung-Ki,Park Jin-Goo
- Jpn J Appl Phys 47(6), 5217-5220, 2008-06-25
- … Imprinting method mechanically transfers patterns from a stamp onto a substrate. … A new micro fabrication method termed electrochemical fabrication (ECF) is introduced to overcome conventional problems of electrical discharge machining (EDM), FeCl3 Wet etching, laser method, electro plating, such as low reliability and reproducibility, high cost. …
- NAID 150000050878
- Development of a New Most-probable-number Method for Enumerating Methanotrophs, Using 48-well Microtiter Plates
- Saitoh Satoshi,Iwasaki Kazuhiro,Yagi Osami
- Microbes and environments 17(4), 191-196, 2002-12
- … We developed a new most-probable-number (MPN) method for enumeration of methanotrophs, using 48-well microtiter plates. … With this method, a microtiter plate which has been inoculated with serial dilutions of a sample is placed in a plastic desiccator containing methane and shaken. … The proposed method gave estimates of population densities of a pure culture of Methylocystis sp. …
- NAID 110001269319
Related Links
- i'm having difficulty in understanding the Replica Plating method.there this question about the isolation of Alanine mutant using this method.as i don't really understand what is Replica Plating method,i hope ...
- Development of a New Most-probable-number Method for Enumerating Methanotrophs, Using 48-well Microtiter Plates Saitoh Satoshi , Iwasaki Kazuhiro , Yagi Osami
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★リンクテーブル★
| リンク元 | 「replica plating」「レプリカ平板法」 |
| 関連記事 | 「method」「plating」「replica」「replica plating」 |
「replica plating」
「レプリカ平板法」
「method」
- n.
- 関
- assay、fashion、law、manner、means、method for measurement、-metry、mode、procedure、process、system、technique、way
「plating」
- n.
「replica」
- n.
- 関
- copy