WordNet

cut a groove into; "chase silver"
a rectangular metal frame used in letterpress printing to hold together the pages or columns of composed type that are printed at one time
pursue someone sexually or romantically (同)chase after
go after with the intent to catch; "The policeman chased the mugger down the alley"; "the dog chased the rabbit" (同)chase after, trail, tail, tag, give chase, dog, go after, track
drive by or as if by pulsation; "A soft breeze pulsed the air"
the rate at which the heart beats; usually measured to obtain a quick evaluation of a persons health (同)pulse_rate, heart_rate
the rhythmic contraction and expansion of the arteries with each beat of the heart; "he could feel the beat of her heart" (同)pulsation, heartbeat, beat
edible seeds of various pod-bearing plants (peas or beans or lentils etc.)
produce or modulate (as electromagnetic waves) in the form of short bursts or pulses or cause an apparatus to produce pulses; "pulse waves"; "a transmitter pulsed by an electronic tube" (同)pulsate
to conduct a test or investigation; "We are experimenting with the new drug in order to fight this disease"
the act of conducting a controlled test or investigation (同)experimentation
a venture at something new or different; "as an experiment he decided to grow a beard"
the testing of an idea; "it was an experiment in living"; "not all experimentation is done in laboratories" (同)experimentation
try something new, as in order to gain experience; "Students experiment sexually"; "The composer experimented with a new style" (同)try out
United States politician and jurist who served as chief justice of the United States Supreme Court (1808-1873) (同)Salmon P. Chase, Salmon Portland Chase
100 puls equal 1 afghani in Afghanistan

PrepTutorEJDIC

〈C〉『追跡』,追撃;追求 / 《the ~》狩猟 / 〈C〉追われる人(動物,船,飛行機) / 〈人・動物など〉'を'『追いかける』,追跡する / (…から)〈人・動物など〉'を'『追い払う』,追い出す《+『名』+『from』(『out of』)+『名』》 / (…を)追う,追跡する《+『after』+『名』》 / 《話》走り回る《+『about』》,(…を)走り回る《+『about』+『名』》
〈金属・木〉‘に'浮き彫りする
(壁・床にある)電線用みぞ
《通例単数形で》『脈はく』 / (一般に,規則的な)鼓動,律動 / (電気の)パルス / (一般的な)傾向 / 脈を打つ,鼓動する
〈U〉《時に複数扱い》(食用の)豆類 / 〈C〉《通例複数形で》豆のとれる植物
(…の)『実験』,試み《+『in』(『on, with』)+『名』》 / (…の)実験をする《+『on(upon, with)』+『名』》

Wikipedia preview

出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2013/08/27 23:10:07」(JST)

wiki en

Pulse-chase analysis of auxin signal transduction in an Arabidopsis thaliana wildtype and an axr2-1 mutant. Wild-type and axr2-1 seedlings were labeled with 35S-methionine, and AXR2/axr2-1 protein was immunoprecipitated either immediately after the labeling period (t = 0) or following a 15-minute chase with unlabeled methionine (t = 15).

In biochemistry and molecular biology, a pulse-chase analysis is a method for examining a cellular process occurring over time by successively exposing the cells to a labeled compound (pulse) and then to the same compound in an unlabeled form (chase). Radioactivity is a commonly used label.

Mechanism[edit source | edit]

A selected cell or a group of cells is first exposed to a labeled compound (the pulse) that is to be incorporated into a molecule or system that is studied (also see pulse labeling). The compound then goes through the metabolic pathways and is used in the synthesis of the product studied. For example, a radioactively labeled form of leucine (³H-leucine) can be supplied to a group of pancreatic B cells, which then uses this amino acid in insulin synthesis.

Shortly after introduction of the labeled compound (usually about 5 minutes, but the actual time needed is dependent on the object studied), excess of the same, but unlabeled, substance (the chase) is introduced into the environment. Following the previous example, the production of insulin would continue, but it would no longer contain the radioactive leucine introduced in the pulse phase and would not be visible using radioactive detection methods. However, the movement of the labeled insulin produced during the pulse period could still be tracked within the cell.

Uses[edit source | edit]

This method is useful for determining the activity of certain cells over a prolonged period of time. The method has been used to study protein kinase C, ubiquitin, and many other proteins. The method was also used to prove the existence and function of Okazaki fragments. George Palade used pulse-chase of radioactive amino acids to elicit the secretory pathway.

References[edit source | edit]

Takahashi M, Ono Y (2003). "Pulse-chase analysis of protein kinase C". Methods Mol. Biol. 233: 163–70. doi:10.1385/1-59259-397-6:163. PMID 12840506.
Ferguson PL, Coombs DH (March 2000). "Pulse-chase analysis of the in vivo assembly of the bacteriophage T4 tail". J. Mol. Biol. 297 (1): 99–117. doi:10.1006/jmbi.2000.3551. PMID 10704310.
Hoyt MA, Zich J, Takeuchi J, Zhang M, Govaerts C, Coffino P (April 2006). "Glycine-alanine repeats impair proper substrate unfolding by the proteasome". EMBO J. 25 (8): 1720–9. doi:10.1038/sj.emboj.7601058. PMC 1440830. PMID 16601692. "Figures and tables — showing Pulse-Chase Analysis"
Alberts, B. (March 2002) Molecular Biology of the Cell, Fourth Edition ISBN 0-8153-3218-1

This molecular biology article is a stub. You can help Wikipedia by expanding it.

UpToDate Contents

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1. 動脈拍動の検討 examination of the arterial pulse
2. 新生児における先天性心疾患（CHD）：重症ＣＨＤの症状およびスクリーニング congenital heart disease chd in the newborn presentation and screening for critical chd
3. 脈圧の上昇 increased pulse pressure
4. パルスオキシメトリ pulse oximetry
5. リウマチ性疾患および腎疾患におけるシクロホスファミドの使用の一般原則 general principles of the use of cyclophosphamide in rheumatic and renal disease

English Journal

Mutations in the fusion protein cleavage site of avian paramyxovirus serotype 2 increase cleavability and syncytium formation but do not increase viral virulence in chickens.

Subbiah M, Khattar SK, Collins PL, Samal SK.SourceVirginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, MD 20742, USA.
Journal of virology.J Virol.2011 Jun;85(11):5394-405. Epub 2011 Mar 30.
Avian paramyxovirus serotype 2 (APMV-2) is one of the nine serotypes of APMV, which infect a wide variety of avian species around the world. In this study, we constructed a reverse genetics system for recovery of infectious recombinant APMV-2 strain Yucaipa (APMV-2/Yuc) from cloned cDNA. The rescued
PMID 21450835

Structural basis of an ERAD pathway mediated by the ER-resident protein disulfide reductase ERdj5.

Hagiwara M, Maegawa K, Suzuki M, Ushioda R, Araki K, Matsumoto Y, Hoseki J, Nagata K, Inaba K.SourceDepartment of Molecular and Cellular Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan.
Molecular cell.Mol Cell.2011 Feb 18;41(4):432-44.
ER-associated degradation (ERAD) is an ER quality-control process that eliminates terminally misfolded proteins. ERdj5 was recently discovered to be a key ER-resident PDI family member protein that accelerates ERAD by reducing incorrect disulfide bonds in misfolded glycoproteins recognized by EDEM1.
PMID 21329881

Japanese Journal

Difference in Carbon Distribution of Initial Photoassimilates between Soybean and Rice as Revealed by 20 s Pulse-300 s Chase Experiments

Okazaki Keiki,Shinano Takuro,Osaki Mitsuru
Soil science and plant nutrition 51(6), 835-840, 2005-10
… Short pulse-chase labeling experiments indicated that there was a considerable difference between soybean and rice in the distribution pattern of 14C to major metabolites during a 300 s chase period. … The distribution of 14C to starch at the end of the chase period was largest in soybean but very small in rice. …
NAID 110004704827

Tissue Distribution of a Major Mevalonate Pyrophosphate Decarboxylase in Rats

MICHIHARA Akihiro,AKASAKI Kenji,YAMORI Yukio,TSUJI Hiroshi
Biological & pharmaceutical bulletin 24(11), 1231-1234, 2001-11-01
… In a pulse-chase experiment using anti-rat 45-kDa MPD antibody, there was no precursor-product relationship between the 45- and the 35-kDa MPD. …
NAID 110003638392