前細胞

関: proto cell

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2015/07/25 23:30:14」(JST)

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The three main structures phospholipids form in solution; the liposome (a closed bilayer), the micelle and the bilayer.

A protocell (or protobiont) is a self-organized, endogenously ordered, spherical collection of lipids proposed as a stepping-stone to the origin of life.^[1]^[2] A central question in evolution is how simple protocells first arose and how they could differ in reproductive output, thus enabling the accumulation of novel biological emergences over time, i.e. biological evolution. Although a functional protocell has not yet been achieved in a laboratory setting, the goal to understand the process appears well within reach.^[3]^[4]^[5]^[6]

1 Overview
2 Selectivity for compartmentalization
- 2.1 Energy gradient
3 Vesicles and micelles
- 3.1 Geothermal ponds and clay
- 3.2 Montmorillonite bubbles
- 3.3 Membrane transport
4 Artificial models
- 4.1 Langmuir-Blodgett deposition
- 4.2 Jeewanu
5 Ethics and controversy
6 See also
7 References
8 External links

Overview

Compartmentalization was important in the origins of life. Membranes create enclosed compartments that are separate from the external environment, thus providing the cell with functionally specialized aqueous spaces. Because lipid bilayer of membranes is impermeable to most hydrophilic molecules (dissolved by water), the cell must have membrane transport systems that are in charge of import of nutritive molecules as well as export of waste.^[7] It is very challenging to construct protocells from molecular assemblies. An important step in this challenge is the achievement of vesicle dynamics that are relevant to cellular functions, such as membrane trafficking and self-reproduction, using amphiphilic molecules. On the primitive Earth, numerous chemical reactions of organic compounds produced the ingredients of life. Of these substances, amphiphilic molecules might be the first player in the evolution from molecular assembly to cellular life.^[8]^[9] A step from vesicle toward protocell might be to develop self-reproducing vesicles coupled with the metabolic system.^[10]

Selectivity for compartmentalization

Self-assembled vesicles are essential components of primitive cells.^[1] The second law of thermodynamics requires that the universe move in a direction in which disorder (or entropy) increases, yet life is distinguished by its great degree of organization. Therefore, a boundary is needed to separate life processes from non-living matter.^[11] The cell membrane is the only cellular structure that is found in all of the cells of all of the organisms on Earth.^[12]

Researchers Irene A. Chen and Jack W. Szostak (Nobel Prize in Physiology or Medicine 2009) amongst others, demonstrated that simple physicochemical properties of elementary protocells can give rise to essential cellular behaviors, including primitive forms of Darwinian competition and energy storage. Such cooperative interactions between the membrane and encapsulated contents could greatly simplify the transition from replicating molecules to true cells.^[4] Furthermore, competition for membrane molecules would favor stabilized membranes, suggesting a selective advantage for the evolution of cross-linked fatty acids and even the phospholipids of today.^[4] This micro-encapsulation allowed for metabolism within the membrane, exchange of small molecules and prevention of passage of large substances across it.^[13] The main advantages of encapsulation include increased solubility of the cargo and creating energy in the form of chemical gradient. Energy is thus often said to be stored by cells in the structures of molecules of substances such as carbohydrates (including sugars), lipids, and proteins, which release energy when chemically combined with oxygen during cellular respiration.^[14]^[15]

Energy gradient

A March 2014 study by NASA's Jet Propulsion Laboratory, demonstrated a unique way to study the origins of life: fuel cells.^[16] Fuel cells are similar to biological cells in that electrons are also transferred to and from molecules. In both cases, this results in electricity and power. The study states that one important factor was that the Earth provides electrical energy at the seafloor. "This energy could have kick-started life and could have sustained life after it arose. Now, we have a way of testing different materials and environments that could have helped life arise not just on Earth, but possibly on Mars, Europa and other places in the Solar System."^[16]

Vesicles and micelles

Scheme of a micelle spontaneously formed by phospholipids in an aqueous solution

When phospholipids are placed in water, the molecules spontaneously arrange such that the tails are shielded from the water, resulting in the formation of membrane structures such as bilayers, vesicles, and micelles.^[2] In modern cells, vesicles are involved in metabolism, transport, buoyancy control,^[17] and enzyme storage. They can also act as natural chemical reaction chambers. A typical vesicle or micelle in aqueous solution forms an aggregate with the hydrophilic "head" regions in contact with surrounding solvent, sequestering the hydrophobic single-tail regions in the micelle centre. This phase is caused by the packing behavior of single-tail lipids in a bilayer. Although the protocellular self-assembly process that spontaneously form lipid monolayer vesicles and micelles in nature resemble the kinds of primordial vesicles or protocells that might have existed at the beginning of evolution, they are not as sophisticated as the bilayer membranes of today's living organisms.^[18]

Rather than being made up of phospholipids, however, early membranes may have formed from monolayers or bilayers of fatty acids, which may have formed more readily in a prebiotic environment.^[19] Fatty acids have been synthesized in laboratories under a variety of prebiotic conditions and have been found on meteorites, suggesting their natural synthesis in nature.^[4]

Oleic acid vesicles represent good models of membrane protocells that could have existed in prebiotic times.^[20]

Geothermal ponds and clay

This fluid lipid bilayer cross section is made up entirely of phosphatidylcholine.

Scientists have come to conclude that life began in hydrothermal vents in the deep sea, but a 2012 study led by Armen Mulkidjanian of Germany's University of Osnabrück, suggests that inland pools of condensed and cooled geothermal vapor have the ideal characteristics for the origin of life.^[21] The conclusion is based mainly on the chemistry of modern cells, where the cytoplasm is rich in potassium, zinc, manganese, and phosphate ions, which are not widespread in marine environments. Such conditions, the researchers argue, are found only where hot hydrothermal fluid brings the ions to the surface — places such as geysers, mud pots, fumaroles and other geothermal features. Within these fuming and bubbling basins, water laden with zinc and manganese ions could have collected, cooled and condensed in shallow pools.^[21]

In the 1990s biochemist James Ferris of Rensselaer Polytechnic Institute showed that montmorillonite clay can help create RNA chains of as many as 50 nucleotides joined together spontaneously into a single RNA molecule.^[5] Then in 2002, Hanczyc, Fujikawa and Szostak discovered that by adding montmorillonite to their solution of fatty acid micelles (lipid spheres), the clay sped up the rate of vesicles formation 100-fold.^[5]

Research has shown that some minerals can catalyze the stepwise formation of hydrocarbon tails of fatty acids from hydrogen and carbon monoxide gases - gases that may have been released from hydrothermal vents or geysers. Fatty acids of various lengths are eventually released into the surrounding water,^[19] but vesicle formation requires a higher concentration of fatty acids, so it is suggested that protocell formation started at land-bound hydrothermal vents such as geysers, mud pots, fumaroles and other geothermal features where water evaporates and concentrates the solute.^[5]^[22]^[23]

Montmorillonite bubbles

A team of applied physicists at Harvard's School of Engineering and Applied Sciences say that primitive cells might have formed inside inorganic clay microcompartments, which can provide an ideal container for the synthesis and compartmentalization of complex organic molecules.^[24] Clay-armored "bubbles" form naturally when particles of montmorillonite clay collect on the outer surface of air bubbles under water. This creates a semi permeable vesicle from materials that are readily available in the environment. The authors remark that montmorillonite is known to serve as a chemical catalyst, encouraging lipids to form membranes and single nucleotides to join into strands of RNA. Primitive reproduction can be envisioned when the clay bubbles burst, releasing the lipid membrane-bound product into the surrounding medium.^[24]

Membrane transport

Schematic showing two possible conformations of the lipids at the edge of a pore. In the top image the lipids have not rearranged, so the pore wall is hydrophobic. In the bottom image some of the lipid heads have bent over, so the pore wall is hydrophilic.

Instead of the more popular phospholipids of modern cells, the membrane of protocells in the RNA world would be composed of fatty acids,^[25] and that such membranes have relatively high permeability to ions and small molecules,^[1] such as nucleoside monophosphate (NMP), nucleoside diphosphate (NDP), and nucleoside triphosphatee (NTP), and may withstand millimolar concentrations of Mg²⁺.^[26] Osmotic pressure also plays a significant role in protocell membrane transport.^[1]

It has been proposed that electroporation resulting from lightning strikes could be a mechanism of natural horizontal gene transfer.^[27] Electroporation is the rapid increase in bilayer permeability induced by the application of a large artificial electric field across the membrane. During electroporation in laboratory procedures, the lipid molecules are not chemically altered but simply shift position, opening up a pore (hole) that acts as the conductive pathway through the bilayer as it is filled with water. The mechanism is the creation of nanometer sized water-filled holes in the membrane. Experimentally, electroporation is used to introduce hydrophilic molecules into cells. It is a particularly useful technique for large highly charged molecules such as DNA and RNA, which would never passively diffuse across the hydrophobic bilayer core.^[28] Because of this, electroporation is one of the key methods of transfection as well as bacterial transformation.

Fusion

Some molecules or particles are too large or too hydrophilic to pass through a lipid bilayer, but can be moved across the cell membrane through fusion or budding of vesicles.^[29] This may have eventually led to mechanisms that facilitate movement of molecules to the inside (endocytosis) or to release its contents into the extracellular space (exocytosis).

Artificial models

Langmuir-Blodgett deposition

Surfactant molecules arranged on an air – water interface

Starting with a technique commonly used to deposit molecules on a solid surface, Langmuir-Blodgett deposition, scientist are able to assemble phospholipid membranes layer by layer of arbitrary complexity.^[30]^[31] These artificial phospholipid membranes support functional insertion both of purified and of in situ expressed membrane proteins.^[31] The technique could help astrobiologists understand how the first living cells originated.^[30]

Jeewanu

The Jeewanu protocells are synthetic chemical particles that possess cell-like structure and seem to have some functional living properties.^[32] First synthesized in 1963 from simple minerals and basic organics while exposed to sunlight, it is still reported to have some metabolic capabilities, the presence of semipermeable membrane, amino acids, phospholipids, carbohydrates and RNA-like molecules.^[32]^[33] However, the nature and properties of the Jeewanu remains to be clarified.^[32]^[33]^[34]

In a similar synthesis experiment using light, led by Jason Dworkin in 2000,^[35] he exposed a frozen mixture of water, methanol, ammonia and carbon monoxide to ultraviolet (UV) radiation. This combination yielded large amounts of organic material that self-organised to form globules or vesicles when immersed in water. Dworkin considered these globules to resemble cell membranes that enclose and concentrate the chemistry of life, separating their interior from the outside world. The globules were between 10 to 40 micrometres (0.00039 to 0.00157 in), or about the size of red blood cells. Remarkably, the globules fluoresced, or glowed, when exposed to UV light. Absorbing UV and converting it into visible light in this way was considered one possible way of providing energy to a primitive cell. If such globules played a role in the origin of life, the fluorescence could have been a precursor to primitive photosynthesis. Such fluorescence also provides the benefit of acting as a sunscreen, diffusing any damage that otherwise would be inflicted by UV radiation. Such a protective function would have been vital for life on the early Earth, since the ozone layer, which blocks out the sun's most destructive UV rays, did not form until after photosynthetic life began to produce oxygen.^[36]

Ethics and controversy

Protocell research has created controversy and opposing opinions, including critics of the vague definition of "artificial life".^[37] The creation of a basic unit of life is the most pressing ethical concern, although the most widespread worry about protocells is their potential threat to human health and the environment through uncontrolled replication.^[38]

References

^ ^a ^b ^c ^d Chen, Irene A.; Walde, Peter (July 2010). "From Self-Assembled Vesicles to Protocells" (PDF). Cold Spring Harb Perspect Biol. 2 (7). doi:10.1101/cshperspect.a002170. PMC 2890201. PMID 20519344.
^ ^a ^b Garwood, Russell J. (2012). "Patterns In Palaeontology: The first 3 billion years of evolution". Palaeontology Online 2 (11): 1–14. Retrieved June 25, 2015.
^ National Science Foundation (2013). "Exploring Life's Origins - Protocells". Retrieved 2014-03-18.
^ ^a ^b ^c ^d Chen, Irene A. (8 December 2006). "The Emergence of Cells During the Origin of Life". Science 314 (5805): 1558–1559. doi:10.1126/science.1137541.
^ ^a ^b ^c ^d Zimmer, Carl (26 June 2004). "What Came Before DNA?". Discover Magazine: 1–5.
^ Rasmussen, Steen (2 July 2014). "Scientists Create Possible Precursor to Life". A Letters Journal Exploring the Frontiers of Physics. Volume 107, Number 2, July 2014. (Astrobiology Web). Retrieved 2014-10-24.
^ Book: "Molecular Biology of the Cell." Alberts B., Johnson Lewis, et al. 2004.
^ Deamer, D.W.; Dworkin, J.P. "Chemistry and Physics of Primitive Membranes." Top. Curr. Chem. 2005, 259, 1–27.
^ Walde, P. "Surfactant Assemblies and their various possible roles for the origin(s) of life." Orig. Life Evol. Biosph. 2006, 36, 109–150.
^ Sakuma, Yuka; Imai, Masayuki (2015). "From Vesicles to Protocells: The Roles of Amphiphilic Molecules". Life 5 (1): 651–675. doi:10.3390/life5010651. Retrieved 2015-04-13.
^ Shapiro, Robert (12 February 2007). "A Simpler Origin for Life". Scientific American.
^ Vodopich, Darrell S.; Moore., Randy (2002). "The Importance of Membranes". Biology Laboratory Manual, 6/a. McGraw-Hill. Retrieved 2014-03-17.
^ Chang, Thomas Ming Swi (2007). Artificial cells : biotechnology, nanomedicine, regenerative medicine, blood substitutes, bioencapsulation, cell/stem cell therapy. Hackensack, N.J.: World Scientific. ISBN 981-270-576-7.
^ Knowles, JR (1980). "Enzyme-catalyzed phosphoryl transfer reactions". Annu. Rev. Biochem. 49: 877–919. doi:10.1146/annurev.bi.49.070180.004305. PMID 6250450.
^ Campbell, Neil A.; Williamson, Brad; Heyden, Robin J. (2006). Biology: Exploring Life. Boston, Massachusetts: Pearson Prentice Hall. ISBN 0-13-250882-6.
^ ^a ^b Clavin, Whitney (13 March 2014). "How Did Life Arise? Fuel Cells May Have Answers". NASA.
^ Walsby, AE (1994). "Gas vesicles". Microbiological reviews 58 (1): 94–144. PMC 372955. PMID 8177173.
^ Szostak, Jack W. (3 September 2004). "Battle of the Bubbles May Have Sparked Evolution". Howard Hughes Medical Institute.
^ ^a ^b National Science Foundation (2013). "Membrane Lipids of Past and Present". Exploring Life's Origins Project - A timeline of Life's Evolution. Retrieved 2014-03-17.
^ Douliez, Jean-Paul; Zhendre, Vanessa; Grélard, Axelle; Dufourc, Erick J. (24 November 2014). "Aminosilane/Oleic Acid Vesicles as Model Membranes of Protocells". Langmuir 30 (49): 14717–14724. doi:10.1021/la503908z. Retrieved 2015-04-13.
^ ^a ^b Switek, Brian (13 February 2012). "Debate bubbles over the origin of life". Nature - News.
^ Szostak, Jack W. (4 June 2008). "Researchers Build Model Protocell Capable of Copying DNA". HHMI News (Howard Hughes Medical Institute).
^ Cohen, Philip (23 October 2003). "Clay's matchmaking could have sparked life". New Scientist. Journal reference: Science (vol 302, p 618 )
^ ^a ^b Stone, Howard A. (7 February 2011). "Clay-armored bubbles may have formed first protocells". Harvard School of Engineering and Applied Sciences.
^ Müller, A. W. (June 2006). "Re-creating an RNA world". Cell Mol Life Sci. 63 (11): 1278–93. doi:10.1007/s00018-006-6047-1.
^ Ma, Wentao; Yu, Chunwu; Zhang, Wentao; Hu., Jiming (Nov 2007). "Nucleotide synthetase ribozymes may have emerged first in the RNA world". RNA 13 (11): 2012–2019. doi:10.1261/rna.658507. PMC 2040096. PMID 17878321. Retrieved 2014-03-18.
^ Demanèche, S; Bertolla, F; Buret, F; et. al (August 2001). "Laboratory-scale evidence for lightning-mediated gene transfer in soil". Appl. Environ. Microbiol. 67 (8): 3440–4. doi:10.1128/AEM.67.8.3440-3444.2001. PMC 93040. PMID 11472916.
^ Neumann, E; Schaefer-Ridder, M; Wang, Y; Hofschneider, PH (1982). "Gene transfer into mouse lyoma cells by electroporation in high electric fields". EMBO J. 1 (7): 841–5. PMC 553119. PMID 6329708.
^ Norris, V.; Raine, D.J. (October 1998). "A fission-fussion origin for life". Orig Life Evol Biosph 28 (4-4): 523–537. PMID 9742727. Retrieved 2014-03-18.
^ ^a ^b "Scientists Create Artificial Cell Membranes". Astrobiology Magazine. 4 October 2014. Retrieved 2014-05-07.
^ ^a ^b Matosevic, Sandro; Paegel, Brian M. (29 September 2013). "Layer-by-layer cell membrane assembly". Nature Chemistry 5 (11): 958–963. doi:10.1038/nchem.1765. Retrieved 2014-05-07.
^ ^a ^b ^c Grote, M (September 2011). "Jeewanu, or the 'particles of life'" (PDF). Journal of Biosciences 36 (4): 563–570. doi:10.1007/s12038-011-9087-0. Archived (PDF) from the original on 2014-03-23.
^ ^a ^b Gupta, V. K.; Rai, R. K. (2013). "Histochemical localisation of RNA-like material in photochemically formed self-sustaining, abiogenic supramolecular assemblies 'Jeewanu'". Int. Res. J. of Science & Engineering 1 (1): 1–4. ISSN 2322-0015.
^ Caren, Linda D.; Ponnamperuma, Cyril (1967). "A review of some experiments on the synthesis of 'Jeewanu'" (PDF). NASA Technical Memorandum X-1439 (Moffett Field, California: Ames Research Center).
^ Dworkin, Jason P.; Deamer, David W.; Sandford, Scott A.; Allamandola, Louis J. (30 January 2001). "Self-assembling amphiphilic molecules: Synthesis in simulated interstellar/precometary ices". Proceedings of the National Academy of Sciences of the United States of America 98 (3): 815–9. Bibcode:2001PNAS...98..815D. doi:10.1073/pnas.98.3.815. PMC 14665. PMID 11158552.
^ Mullen, L (5 September 2005). "Building Life from Star-Stuff". Astrobiology Magazine.
^ "Life after the synthetic cell". Nature 465 (7297): 422–424. 27 May 2010. doi:10.1038/465422a. PMID 20495545.
^ Beadau, Mark A.; Parke, Emily C. (2009). The ethics of protocells moral and social implications of creating life in the laboratory (Online ed.). Cambridge, Mass.: MIT Press. ISBN 978-0-262-51269-5.

External links

"Protocells: Bridging Nonliving and Living Matter." Edited by Steen Rasmussen, Mark A. Bedau, Liaochai Chen, David Deamer, David Krakauer, Norman, H.Packard and Peter F. Stadler. MIT Press, Cambridge, Massachusetts. 2008.

"Living Chemistry & A Natural History of Protocells." Synth-ethic: Art and Synthetic Biology Exhibition (2013) at the Natural History Museum, Vienna, Austria.

English Journal

Antioxidant effects of gastrointestinal digested purple carrot extract on the human cells of colonic mucosa.

Olejnik A1, Rychlik J2, Kidoń M3, Czapski J3, Kowalska K2, Juzwa W2, Olkowicz M2, Dembczyński R2, Moyer MP4.
Food chemistry.Food Chem.2016 Jan 1;190:1069-77. doi: 10.1016/j.foodchem.2015.06.080. Epub 2015 Jun 23.
Purple carrot (PC) is a potential dietary constituent, which represents a valuable source of antioxidants and can modulate the reactive oxygen species (ROS) level in the gastrointestinal tract. Antioxidant capacity of a PC extract subjected to digestion process simulated in the artificial alimentary
PMID 26213078

Cell Cycle Synchronization Using a Microfluidic Synchronizer for Fission Yeast Cells.

Wang S1, Luo C.
Methods in molecular biology (Clifton, N.J.).Methods Mol Biol.2016;1342:259-68. doi: 10.1007/978-1-4939-2957-3_15.
To produce synchronized cell colonies, many cell cycle synchronization technologies have been developed, among which the baby machine may be considered the most artifact-free. Baby machines incubate "mother cells" under normal conditions and collects their "babies," producing cell cultures that are
PMID 26254929

Whole-cell biosensor for label-free detection of GPCR-mediated drug responses in personal cell lines.

Hillger JM1, Schoop J1, Boomsma DI2, Eline Slagboom P3, IJzerman AP1, Heitman LH4.
Biosensors & bioelectronics.Biosens Bioelectron.2015 Dec 15;74:233-42. doi: 10.1016/j.bios.2015.06.031. Epub 2015 Jun 23.
Deciphering how genetic variation in drug targets such as G protein-coupled receptors (GPCRs) affects drug response is essential for precision medicine. GPCR signaling is traditionally investigated in artificial cell lines which do not provide sufficient physiological context. Patient-derived cell l
PMID 26143464

Japanese Journal

ベシクルからプロトセルヘのアプローチ (特集境界領域シンポジウム「膜解析の最前線 : 生体膜・膜タンパク質から模擬膜,ソフトマターまで)

佐久間由香,今井正幸
膜 = Membrane 39(5), 316-321, 2014-09
NAID 40020215588

Molecular Building Blocks and Their Architecture in Biologically/Environmentally Compatible Soft Matter Chemical Machinery

, , [他], , , , ,
Journal of Oleo Science 63(11), 1085-1098, 2014
… If the degradation or dissipation of soft matter molecular building blocks and biomaterial molecules/polymers can be achieved, soft matter particles composed of them can be used to realize chemical machinery such as selfpropelled droplets, drug delivery carriers, tissue regeneration scaffolds, protocell models, cell-/tissuemarkers, and molecular computing systems. …
NAID 130004700271

脂質膜のダイナミクスからみる細胞の起源

鈴木宏明
生物物理 53(3), 134-139, 2013-05-25
… The first cell on earth, or the protocell, is thought to be a very primitive existence; … The proliferation and evolution of the protocell should have been achieved by self-reproduction of the informational molecules as well as that of the membrane structure. …
NAID 10031159064

Related Pictures

★リンクテーブル★

リンク元	「前細胞」「proto cell」

「前細胞」

　　[★]

英: protocell、proto cell

「proto cell」

　　[★]

前細胞

関: protocell

[Chen_2010-1] Chen, Irene A.; Walde, Peter (July 2010). "From Self-Assembled Vesicles to Protocells" (PDF). Cold Spring Harb Perspect Biol. 2 (7). doi:10.1101/cshperspect.a002170. PMC 2890201. PMID 20519344.

[PiP-2] Garwood, Russell J. (2012). "Patterns In Palaeontology: The first 3 billion years of evolution". Palaeontology Online 2 (11): 1–14. Retrieved June 25, 2015.

[Exploring-3] National Science Foundation (2013). "Exploring Life's Origins - Protocells". Retrieved 2014-03-18.

[Chen_2006-4] Chen, Irene A. (8 December 2006). "The Emergence of Cells During the Origin of Life". Science 314 (5805): 1558–1559. doi:10.1126/science.1137541.

[Discover_2004-5] Zimmer, Carl (26 June 2004). "What Came Before DNA?". Discover Magazine: 1–5.

[6] Rasmussen, Steen (2 July 2014). "Scientists Create Possible Precursor to Life". A Letters Journal Exploring the Frontiers of Physics. Volume 107, Number 2, July 2014. (Astrobiology Web). Retrieved 2014-10-24.

[7] Book: "Molecular Biology of the Cell." Alberts B., Johnson Lewis, et al. 2004.

[8] Deamer, D.W.; Dworkin, J.P. "Chemistry and Physics of Primitive Membranes." Top. Curr. Chem. 2005, 259, 1–27.

[9] Walde, P. "Surfactant Assemblies and their various possible roles for the origin(s) of life." Orig. Life Evol. Biosph. 2006, 36, 109–150.

[10] Sakuma, Yuka; Imai, Masayuki (2015). "From Vesicles to Protocells: The Roles of Amphiphilic Molecules". Life 5 (1): 651–675. doi:10.3390/life5010651. Retrieved 2015-04-13.

[SciAm_2007-11] Shapiro, Robert (12 February 2007). "A Simpler Origin for Life". Scientific American.

[mgh.ex9-12] Vodopich, Darrell S.; Moore., Randy (2002). "The Importance of Membranes". Biology Laboratory Manual, 6/a. McGraw-Hill. Retrieved 2014-03-17.

[Chang_2007-13] Chang, Thomas Ming Swi (2007). Artificial cells : biotechnology, nanomedicine, regenerative medicine, blood substitutes, bioencapsulation, cell/stem cell therapy. Hackensack, N.J.: World Scientific. ISBN 981-270-576-7.

[Knowles_1980-14] Knowles, JR (1980). "Enzyme-catalyzed phosphoryl transfer reactions". Annu. Rev. Biochem. 49: 877–919. doi:10.1146/annurev.bi.49.070180.004305. PMID 6250450.

[pph.2006-15] Campbell, Neil A.; Williamson, Brad; Heyden, Robin J. (2006). Biology: Exploring Life. Boston, Massachusetts: Pearson Prentice Hall. ISBN 0-13-250882-6.

[NASA-20140313-16] Clavin, Whitney (13 March 2014). "How Did Life Arise? Fuel Cells May Have Answers". NASA.

[mr.58-17] Walsby, AE (1994). "Gas vesicles". Microbiological reviews 58 (1): 94–144. PMC 372955. PMID 8177173.

[HHMI_2004-18] Szostak, Jack W. (3 September 2004). "Battle of the Bubbles May Have Sparked Evolution". Howard Hughes Medical Institute.

[Exploring_Origins-19] National Science Foundation (2013). "Membrane Lipids of Past and Present". Exploring Life's Origins Project - A timeline of Life's Evolution. Retrieved 2014-03-17.

[20] Douliez, Jean-Paul; Zhendre, Vanessa; Grélard, Axelle; Dufourc, Erick J. (24 November 2014). "Aminosilane/Oleic Acid Vesicles as Model Membranes of Protocells". Langmuir 30 (49): 14717–14724. doi:10.1021/la503908z. Retrieved 2015-04-13.

[Switek_2012-21] Switek, Brian (13 February 2012). "Debate bubbles over the origin of life". Nature - News.

[HHMI_protocell_model-22] Szostak, Jack W. (4 June 2008). "Researchers Build Model Protocell Capable of Copying DNA". HHMI News (Howard Hughes Medical Institute).

[ns.dn4307-23] Cohen, Philip (23 October 2003). "Clay's matchmaking could have sparked life". New Scientist. Journal reference: Science (vol 302, p 618 )

[Harvard_2011-24] Stone, Howard A. (7 February 2011). "Clay-armored bubbles may have formed first protocells". Harvard School of Engineering and Applied Sciences.

[cmls.63-25] Müller, A. W. (June 2006). "Re-creating an RNA world". Cell Mol Life Sci. 63 (11): 1278–93. doi:10.1007/s00018-006-6047-1.

[Chunwu_2007-26] Ma, Wentao; Yu, Chunwu; Zhang, Wentao; Hu., Jiming (Nov 2007). "Nucleotide synthetase ribozymes may have emerged first in the RNA world". RNA 13 (11): 2012–2019. doi:10.1261/rna.658507. PMC 2040096. PMID 17878321. Retrieved 2014-03-18.

[Deman.C3.A8che2001-27] Demanèche, S; Bertolla, F; Buret, F; et. al (August 2001). "Laboratory-scale evidence for lightning-mediated gene transfer in soil". Appl. Environ. Microbiol. 67 (8): 3440–4. doi:10.1128/AEM.67.8.3440-3444.2001. PMC 93040. PMID 11472916.

[Neumann1982-28] Neumann, E; Schaefer-Ridder, M; Wang, Y; Hofschneider, PH (1982). "Gene transfer into mouse lyoma cells by electroporation in high electric fields". EMBO J. 1 (7): 841–5. PMC 553119. PMID 6329708.

[oleb.28-29] Norris, V.; Raine, D.J. (October 1998). "A fission-fussion origin for life". Orig Life Evol Biosph 28 (4-4): 523–537. PMID 9742727. Retrieved 2014-03-18.

[Artificial_cell_membranes-30] "Scientists Create Artificial Cell Membranes". Astrobiology Magazine. 4 October 2014. Retrieved 2014-05-07.

[Matosevic_2014-31] Matosevic, Sandro; Paegel, Brian M. (29 September 2013). "Layer-by-layer cell membrane assembly". Nature Chemistry 5 (11): 958–963. doi:10.1038/nchem.1765. Retrieved 2014-05-07.

[Grote_2011-32] Grote, M (September 2011). "Jeewanu, or the 'particles of life'" (PDF). Journal of Biosciences 36 (4): 563–570. doi:10.1007/s12038-011-9087-0. Archived (PDF) from the original on 2014-03-23.

[Gupta_2013-33] Gupta, V. K.; Rai, R. K. (2013). "Histochemical localisation of RNA-like material in photochemically formed self-sustaining, abiogenic supramolecular assemblies 'Jeewanu'". Int. Res. J. of Science & Engineering 1 (1): 1–4. ISSN 2322-0015.

[NASA_1967-34] Caren, Linda D.; Ponnamperuma, Cyril (1967). "A review of some experiments on the synthesis of 'Jeewanu'" (PDF). NASA Technical Memorandum X-1439 (Moffett Field, California: Ames Research Center).

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[StarStuff-36] Mullen, L (5 September 2005). "Building Life from Star-Stuff". Astrobiology Magazine.

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