High-Resolution Genome-Wide Mapping of Nucleosome Positioning and Occupancy Level Using Paired-End Sequencing Technology.
Brunelle M1, Rodrigue S1, Jacques PÉ1,2,3, Gévry N4.
Methods in molecular biology (Clifton, N.J.).Methods Mol Biol.2017;1528:229-243.
Because of its profound influence on DNA accessibility for protein binding and thus on the regulation of diverse biological processes, nucleosome positioning has been studied for many years. In the past decade, high-throughput sequencing technologies have opened new perspectives in this research fie
Analytical Ultracentrifuge Analysis of Nucleosomes Assembled from Recombinant, Acid-Extracted, HPLC-Purified Histones.
Cheema MS1, Ausió J2.
Methods in molecular biology (Clifton, N.J.).Methods Mol Biol.2017;1528:75-95.
The accumulating discoveries of new posttranslational modifications (PTMs) and the increasing relevance of histone variants within the frame of epigenetics demand the availability of methods for a rapid and efficient nucleosome reconstitution to analyze their structural and functional implications.
Histone Purification from Saccharomyces cerevisiae.
Jourquin F1, Géli V2.
Methods in molecular biology (Clifton, N.J.).Methods Mol Biol.2017;1528:69-73.
The nucleosome structure consists of a histone octamer made by a tetramer of H3-H4 histones and two dimers of H2A-H2B. Nucleosomes undergo extensive posttranslational modifications that regulate nucleosome interactions, position, and stability.We describe a protocol allowing the robust purification
Thermal denaturation and template activities of reconstituted DNA-histone complexes
Oda Takuzo,Omura Sachiko,Hidaka Hideyuki
Acta Medica Okayama 31(5), 1977-10
… The thermal denaturation profile and nuclease digestion pattern of DNA-histone H2A, H2B, H3 and H4 complex were compatible with those of the polynucleosome structure of chromatin. …
3] U/[micro]L MNase, where the bands indicative of polynucleosomes (larger than 4mers and 3-mers, respectively) were missing from FA-treated cells (arrowheads ... Disclaimer All content on this website, including dictionary ...
polynucleosome (plural polynucleosomes) A series of many nucleosomes Retrieved from "https://en.wiktionary.org/w/index.php?title=polynucleosome&oldid=39478829" Categories: English words prefixed with poly- English nouns ...