WordNet

perennial of the genus Phytolacca
an agent that triggers mitosis

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2015/10/15 15:08:15」(JST)

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1. 小児での潜在的に毒性を持つ植物の摂取による臨床症状および評価 clinical manifestations and evaluation of potentially toxic plant ingestions in children
2. 体液性免疫応答 the humoral immune response
3. 免疫系の臨床検査評価 laboratory evaluation of the immune system
4. 小児における分類不能型免疫不全症 common variable immunodeficiency in children
5. ディジョージ症候群：評価、診断、および管理 digeorge syndrome evaluation diagnosis and management

English Journal

Optimization of interferon gamma ELISPOT assay to detect human cytomegalovirus specific T-cell responses in solid organ transplants.

Abate D1, Saldan A2, Forner G2, Tinto D2, Bianchin A2, Palù G2.Author information 1Department of Molecular Medicine, University of Padua School of Medicine, via Gabelli, 63-35121 Padova, Italy. Electronic address: davide.abate@unipd.it.2Department of Molecular Medicine, University of Padua School of Medicine, via Gabelli, 63-35121 Padova, Italy.AbstractAssessing the CMV specific CMI in transplant subjects represents a promising strategy to determine the risk of infection on individual basis. In this study 61 adult CMV IgG seropositive solid organ transplant recipients were examined in order to improve the efficacy of CMI detection. For this purpose, pair-wise comparisons were conducted comparing positive control stimuli PWM and PMA/iono and CMV stimuli, pp65 peptide pool and whole CMV particle. Rosette pre-depletion of blood was also investigated for detecting CD4+ or CD8+ T-cell responses using the IFN-g ELISPOT assay. In the time-points 30-180 days after transplantation, PMA/iono produced statistically significant higher responses compared to PWM, probably because PMA/iono activation pathway is independent from the effect of immunosuppressive drugs. The data showed that 11% of transplant patients displayed very low or undetectable responses to pp65 peptide pool antigen while having sustained high responses to whole CMV particle. In addition, in all the subjects analyzed, CMI responses to CMV particle produced a statistically significant higher number of spots compared to pp65 peptide pool antigen. Rosette pre-depletion of whole blood proved to be effective in detecting CD4+ or CD8+ T-cell responses similarly to flow cytometry. Taken together, the following recommendations are suggested to optimize the CMV-ELISPOT for transplantation settings: (1) use PMA/iono as positive control; (2) whole virus particle should be used to avoid peptide-related false negative responses; (3) a rosette pre-depletion step may be useful to detect CD4+ or CD8+ T-cell responses.
Journal of virological methods.J Virol Methods.2014 Feb;196:157-62. doi: 10.1016/j.jviromet.2013.10.036. Epub 2013 Nov 8.
Assessing the CMV specific CMI in transplant subjects represents a promising strategy to determine the risk of infection on individual basis. In this study 61 adult CMV IgG seropositive solid organ transplant recipients were examined in order to improve the efficacy of CMI detection. For this purpos
PMID 24216234

The pattern recognition molecule ficolin-1 exhibits differential binding to lymphocyte subsets, providing a novel link between innate and adaptive immunity.

Genster N, Ma YJ, Munthe-Fog L, Garred P.Author information Laboratory of Molecular Medicine, Department of Clinical Immunology, Section 7631, Rigshospitalet, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark.AbstractFicolin-1 is a soluble pattern recognition molecule synthesized by myeloid cells and capable of activating the lectin pathway of complement on the surface of pathogens. It is tethered to the membranes of monocytes and granulocytes; however, the biological significance of cell-associated ficolin-1 is unknown. Recognition of healthy host cells by a pattern recognition molecule constitutes a potential hazard to self cells and tissues, emphasizing the importance of further elucidating the reported self-recognition. In the current study we investigated the potential recognition of lymphocytes by ficolin-1 and demonstrated that CD56(dim) NK-cells and both CD4(+) and CD8(+) subsets of activated T-cells were recognized by ficolin-1. In contrast we did not detect binding of ficolin-1 to CD56(bright) NK-cells, NKT-cells, resting T-cells or B-cells. Furthermore, we showed that the protein-lymphocyte interaction occurred via the pathogen-recognition domain of ficolin-1 to sialic acid on the cell surface. Thus, the differential binding of ficolin-1 to lymphocyte subsets suggests ficolin-1 as a novel link between innate and adaptive immunity. Our results provide new insight about the recognition properties of ficolin-1 and point toward additional immune modulating functions of the molecule besides its role in pathogen recognition.
Molecular immunology.Mol Immunol.2014 Feb;57(2):181-90. doi: 10.1016/j.molimm.2013.09.006. Epub 2013 Oct 23.
Ficolin-1 is a soluble pattern recognition molecule synthesized by myeloid cells and capable of activating the lectin pathway of complement on the surface of pathogens. It is tethered to the membranes of monocytes and granulocytes; however, the biological significance of cell-associated ficolin-1 is
PMID 24161415

Lack of expression of an alternative CD20 transcript variant in circulating B cells from patients with pemphigus.

Gamonet C, Ferrand C, Colliou N, Musette P, Joly P, Girardin M, Humbert P, Aubin F.Author information Inserm UMR 1098, Etablissement Français du Sang Bourgogne-Franche Comté, Besançon, France.AbstractWe have identified a spliced mRNA transcript of CD20 (named D393-CD20) which was associated with resistance to RTX in primary B cell from patients with lymphoma and leukaemia. In the present work, we wished to investigate whether D393-CD20 variant was expressed by B cells from patients with pemphigus. Ten patients with bullous pemphigoid and twenty-five patients with pemphigus were included. All patients were responder to conventional immunosuppressive agents or rituximab (n = 11). Efficacy of B-cell activation by pokeweed mitogen was assessed by CD86 expression using a FACS Canto II flow cytometer. mRNA CD20 expression study was then performed using RT-PCR assay allowing first to discriminate wild-type (wt)-CD20 and D393-CD20 transcript. Although wt-CD20 expression was always detected, we were unable to detect D393-CD20, even after B-cell activation or RTX treatment. Our results suggest that D393-CD20 transcript may be a molecular marker of B-cell malignancies rather than autoimmune disease like pemphigus. Further study of RTX non-responders or non-escaping PV patients is thus still required to appreciate whether D393-CD20 expression may be detected under the pressure of RTX therapy.
Experimental dermatology.Exp Dermatol.2014 Jan;23(1):66-7. doi: 10.1111/exd.12299.
We have identified a spliced mRNA transcript of CD20 (named D393-CD20) which was associated with resistance to RTX in primary B cell from patients with lymphoma and leukaemia. In the present work, we wished to investigate whether D393-CD20 variant was expressed by B cells from patients with pemphigu
PMID 24313590

Japanese Journal

ニホンウズラ(Coturnix japonica)末梢血リンパ球活性化における4種のマイトージェンの培養至適濃度

原ひろみ,半澤惠,吉田豊,渡邉誠喜
東京農業大学農学集報 55(2), 108-114, 2010-09-17
ニホンウズラの末梢血リンパ球活性化におけるConA, PHA-L, PHA-MとPWMのマイトージェンの至適培養濃度を検討した。末梢血よりリンパ球をPercoll密度勾配遠心法により分離し,2×106/ml浮遊液に調製し,各マイトージェンを0〜1,000μg/ml添加したRPMI1640培地(血清不含)で0,24,48,72時間培養した。各培養時間後にMTTアッセイにより細胞活性を測定し,培養0時 …
NAID 110007666417

乳牛の末梢血液中のリンパ球幼若化能に及ぼすBacillus subtilis菌体製剤の影響

佐藤繁,小野秀弥,一條俊浩,岡田啓司
日本家畜臨床学会誌 29(1), 6-9, 2006
健康乳牛にBacillus subtilis菌体製剤を経口投与し、末梢血液中のリンパ球幼若化能の推移を検討した。リンパ球幼若化能は50gの5日間あるいは250gの1回投与牛のいずれも、初回投与日から投与後2-3日目にかけて上昇し、その後しだいに低下したが、7日目にも投与前に比べて高値(ConA、PWM)を示した。一方、50g28日間投与牛のリンパ球幼若化能は、投与開始時から投与後3-4週目にかけて …
NAID 130001005967