WordNet

amide combining the amino group of one amino acid with the carboxyl group of another; usually obtained by partial hydrolysis of protein

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A signal "on" photoelectrochemical biosensor for assay of protein kinase activity and its inhibitor based on graphite-like carbon nitride, Phos-tag and alkaline phosphatase.

Yin H1, Sun B2, Dong L1, Li B1, Zhou Y3, Ai S4.
Biosensors & bioelectronics.Biosens Bioelectron.2015 Feb 15;64:462-8. doi: 10.1016/j.bios.2014.09.070. Epub 2014 Oct 4.
A highly sensitive and selective photoelectrochemical (PEC) biosensor is fabricated for the detection of protein kinase activity based on visible-light active graphite-like carbon nitride (g-C3N4) and the specific recognition utility of Phos-tag for protein kinase A (PKA)-induced phosphopeptides. Fo
PMID 25286353

Label-free fluorescence assay for protein kinase based on peptide biomineralized gold nanoclusters as signal sensing probe.

Song W1, Wang Y1, Liang RP1, Zhang L1, Qiu JD2.
Biosensors & bioelectronics.Biosens Bioelectron.2015 Feb 15;64:234-40. doi: 10.1016/j.bios.2014.08.082. Epub 2014 Sep 6.
A label-free, sensitive and simple method to detect protein kinase based on the selective aggregation of phosphorylated peptide-gold nanoclusters (peptide-AuNCs) triggered by Zr(4+) ion coordination is developed. The AuNCs were synthesized by peptide without any strong reducing agents, which prevent
PMID 25222326

Characterization of anti-P monoclonal antibodies directed against the ribosomal protein-RNA complex antigen and produced using Murphy Roths large autoimmune-prone mice.

Sato H1, Onozuka M, Hagiya A, Hoshino S, Narita I, Uchiumi T.
Clinical and experimental immunology.Clin Exp Immunol.2015 Feb;179(2):236-44. doi: 10.1111/cei.12460.
Autoantibodies, including anti-ribosomal P proteins (anti-P), are thought to be produced by an antigen-driven immune response in systemic lupus erythematosus (SLE). To test this hypothesis, we reconstituted the ribosomal antigenic complex in vitro using human P0, phosphorylated P1 and P2 and a 28S r
PMID 25255895

Stimulation of CK2-dependent Grp94 phosphorylation by the nuclear localization signal peptide.

Miyata Yoshihiko,Yoneda Yoshihiro,Yahara Ichiro
Molecular and cellular biochemistry 356(1-2), 191-200, 2011-10
… In this study, we investigated effects of the NLS-peptide of Large T antigen on protein phosphorylation. … When crude cell lysates were incubated with [γ-(32)P]ATP, phosphorylation of several endogenous substrates with molecular masses of 100, 80, 50, and 45 kDa by an endogenous kinase was stimulated by the addition of the wild type NLS-peptide (CPKKKRKVEDP). …
NAID 80022043429

A manual sequence method of peptides and phosphopeptides using 4-(1'-cyanoisoindolyl)phenylisothiocyanate.

Shibata Takayuki,Wainaina Moses N,Miyoshi Takayuki,Kabashima Tsutomu,Kai Masaaki
Journal of Chromatography. A 1218(24), 3757-3762, 2011-06-17
… Up to the 4th residue of phosphorylated pentapeptides was successfully identified, whereas phosphoamino acid residues could not be detected by the conventional procedure using phenylisothiocyanate (PITC). … The results demonstrated the potential of CIPIC as a derivatization reagent for peptide sequencing and the applicability of the method for the study and identification of phosphoamino acids in peptides. …
NAID 120003220737