WordNet

any of a large group of nitrogenous organic compounds that are essential constituents of living cells; consist of polymers of amino acids; essential in the diet of animals for growth and for repair of tissues; can be obtained from meat and eggs and milk and legumes; "a diet high in protein"

PrepTutorEJDIC

全文を閲覧するには購読必要です。 To read the full text you will need to subscribe.

Efficient expression, purification and characterization of native human cystatin C in Escherichia coli periplasm.

Zhou Y1, Zhou Y2, Li J1, Chen J1, Yao Y1, Yu L1, Peng D3, Wang M3, Su D1, He Y4, Gou L5.
Protein expression and purification.Protein Expr Purif.2015 Jul;111:18-22. doi: 10.1016/j.pep.2015.03.006. Epub 2015 Mar 17.
Human cystatin C (HCC), encoded by cystatin 3 gene, is a 13.3kDa endogenous cysteine proteinase inhibitor and an important biomarker of renal function. However, expressing recombinant cystatin C is difficult because of low yield and inclusion bodies in Escherichia coli (E. coli). In this study, we c
PMID 25795130

Hatayama K1, Ide T2.
Protein expression and purification.Protein Expr Purif.2015 Jul;111:1-8. doi: 10.1016/j.pep.2015.03.005. Epub 2015 Mar 17.
Recombinant human erythropoietin receptor (rhEPOR) has applicability as an affinity ligand for purification of recombinant human erythropoietin (rHuEPO) because of its specific binding to rHuEPO. For application of rhEPOR as a ligand for purification of rHuEPO, soluble rhEPOR was expressed in the pe
PMID 25795089

Functional analysis of the N-terminal region of endolysin Lyb5 encoded by Lactobacillus fermentum bacteriophage φPYB5.

Guo T1, Zhang C2, Liu W2, Wang S2, Kong J3.
International journal of food microbiology.Int J Food Microbiol.2015 Jun 16;203:1-7. doi: 10.1016/j.ijfoodmicro.2015.02.033. Epub 2015 Mar 3.
Lactobacillus fermentum temperate bacteriophage φPYB5 uses endolysin Lyb5 and holin Hyb5 to burst the host cell. Previous results showed that expression of Lyb5 in Escherichia coli caused host cell lysis slowly, leading us to suppose that Lyb5 could pass the cytoplasmic membrane partly. In this wor
PMID 25770427