WordNet
- a unit of geological time during which a system of rocks formed; "ganoid fishes swarmed during the earlier geological periods" (同)geological period
- (ice hockey) one of three divisions into which play is divided in hockey games
- the end or completion of something; "death put a period to his endeavors"; "a change soon put a period to my tranquility"
- the interval taken to complete one cycle of a regularly repeating phenomenon
- a punctuation mark (.) placed at the end of a declarative sentence to indicate a full stop or after abbreviations; "in England they call a period a stop" (同)point, full_stop, stop, full point
- (microscopy) a dye or other coloring material that is used in microscopy to make structures visible
- color for microscopic study; "The laboratory worker dyed the specimen"
- color with a liquid dye or tint; "Stain this table a beautiful walnut color"; "people knew how to stain glass a beautiful blue in the middle ages"
- produce or leave stains; "Red wine stains the table cloth"
- a soiled or discolored appearance; "the wine left a dark stain" (同)discoloration, discolouration
- street name for lysergic acid diethylamide (同)back breaker, battery-acid, dose, dot, Elvis, loony toons, Lucy in the sky with diamonds, pane, superman, window pane, Zen
- any of various water-soluble compounds having a sour taste and capable of turning litmus red and reacting with a base to form a salt
- having the characteristics of an acid; "an acid reaction"
- happening or recurring at regular intervals; "the periodic appearance of the seventeen-year locust" (同)periodical
- recurring or reappearing from time to time; "periodic feelings of anxiety" (同)occasional
- marked or dyed or discolored with foreign matter; "a badly stained tablecloth"; "tear-stained cheeks"
- having a coating of stain or varnish (同)varnished
PrepTutorEJDIC
- (あることが続く)『期間』 / (歴史的な)『時代』 / (試合の)『一区切り』,ピリオド / 授業時間,時限: / (循環して起こる現象などの)周期 / (回転運動などの)周期 / 紀(地質時代の区分の一つ;era(代)の下,epoch(世)の上) / 《おもに米》『ピリオド』,終止符(《英》full stop;Mr.,U.S.など略語の符号(.)も含む) / 《複数形で》《文》掉尾(とうび)文(多くの節から成り,論理的に緊密な構成をもっている文) / 《名詞の前にのみ用いて》ある時代[特有]の,時代物の / (文の終わりに感嘆詞的に)以上,終わり・月経(期間)
- (…で)…‘を'『汚す』,‘に'しみをつける《+『名』+『with』+『名』》 / 〈ガラス・木材・布など〉‘に'『着色する』 / (…で)〈人格・名声など〉‘を'汚す,‘に'傷をつける《+『名』+『with』+『名』》 / 汚れる,しみがつく / 〈C〉〈U〉(…についた)『しみ』,汚れ《+『on』+『名』》 / 〈U〉〈C〉着色剤,染料 / 〈C〉(人格・名声などに対する)汚点,傷《+『on』(『upon』)+『名』》
- 酸性の / 酸味のある,すっぱい(sour) / (言葉・態度などが)厳しい,しんらつな / 酸 / すっぱいもの / 《俗》=LSD
- 定期的な,周期的な / ときぎき起こる: / 掉尾(とうび)的の:
Wikipedia preview
出典(authority):フリー百科事典『ウィキペディア(Wikipedia)』「2015/04/07 00:31:51」(JST)
[Wiki en表示]
Periodic acid–Schiff (PAS) is a staining method used to detect polysaccharides such as glycogen, and mucosubstances such as glycoproteins, glycolipids and mucins in tissues. The reaction of periodic acid oxidizes the vicinal diols in these sugars, usually breaking up the bond between two adjacent carbons not involved in the glycosidic linkage or ring closure in the ring of the monosaccharide units that are parts of the long polysaccharides, and creating a pair of aldehydes at the two free tips of each broken monosaccharide ring. The oxidation condition has to be sufficiently regulated so as to not oxidize the aldehydes further. These aldehydes then react with the Schiff reagent to give a purple-magenta color. A suitable basic stain is often used as a counterstain.
PAS diastase stain (PAS-D) is PAS stain used in combination with diastase, an enzyme that breaks down glycogen. Alcian blue/periodic acid–Schiff (AB/PAS or AB-PAS) uses alcian blue before the PAS step.
Contents
- 1 Uses
- 2 See also
- 3 References
- 4 External links
Uses
Gastric signet ring cell carcinoma histopathology, PAS stain.
Esophageal candidiasis, PAS stain.
Liver in glycogen storage disease, PAS stain
PAS staining is mainly used for staining structures containing a high proportion of carbohydrate macromolecules (glycogen, glycoprotein, proteoglycans), typically found in e.g. connective tissues, mucus, the glycocalyx, and basal laminae.
PAS staining can be used to assist in the diagnosis of several medical conditions:
- Glycogen storage disease (versus other storage disorders).
- Adenocarcinomas, which often secrete neutral mucins.
- Paget disease of the breast.[1]
- Alveolar soft part sarcoma.[2]
- Staining macrophages in Whipple's disease.[3]
- It can be used to diagnose α1-antitrypsin deficiency if periportal liver hepatocytes stain positive.
- Aggregates of PAS positive lymphocytes are present in epidermis in Mycosis fungoides and Sezary syndrome, called Pautrier microabscesses.
- Erythroleukemia, a leukemia of immature red blood cells. These cells stain a bright fuchsia.
- Pulmonary alveolar proteinosis.
- Fungal infection, the cell walls of fungi stain magenta; this only works on living fungi. In contrast, Grocott's methenamine silver stain (GMS) will stain both living and dead fungal organisms.
- It is used to identify glycogen in lung biopsy specimens of infants with pulmonary interstitial glycogenosis (PIG).
- It can be use to highlight super cross-linked lipids inclusions in ceroid lipofuscinosis (NCL).
Presence of glycogen can be confirmed on a section of tissue by using diastase to digest the glycogen from a section, then comparing a diastase digested PAS section with a normal PAS section. The diastase negative slide will show a magenta staining where glycogen is present within a section of tissue. The slide that has been treated with diastase will lack any positive PAS staining in those locations on the slide
PAS staining is also used for staining cellulose. One example would be looking for implanted medical devices composed of nonoxidized cellulose.
If the PAS stain will be performed on tissue, the recommended fixative is 10% neutral-buffered formalin or Bouin solution. For blood smears, the recommended fixative is methanol. Glutaraldehyde is not recommended because free aldehyde groups may be available to react with the Schiff reagent, which may result in false positive staining.[4]
See also
- Methyl violet
- Prussian blue
- Egyptian Blue
- Methyl blue
- Methylene blue
- New methylene blue
- Han Purple
- Potassium ferrocyanide
- Potassium ferricyanide
- Gentian violet
- Eosin
- Fluorescein
- Carboxyfluorescein
- Fluorescein isothiocyanate (FITC)
- Fluorescein amidite (FAM)
- Erythrosine
- Rose Bengal
- DyLight Fluor, a product line of fluorescent dyes
- Fluorescein diacetate hydrolysis, a biochemistry laboratory test
- Laser dyes
References
- ^ Thomas J. Lawton (27 April 2009). Breast. Cambridge University Press. pp. 55–. ISBN 978-0-521-88159-3. Retrieved 16 November 2010.
- ^ (Ladanyi et al 2002
- ^ C. Hauser (29 August 2005). Mayo Clinic Gastroenterology and Hepatology Board Review. CRC Press. pp. 108–. ISBN 978-0-203-50274-7. Retrieved 16 November 2010.
- ^ Carson, Freida L.; Hladik, Christa (2009). Histotechnology: A Self-Instructional Text (3 ed.). Hong Kong: American Society for Clinical Pathology Press. pp. 137–139. ISBN 978-0-89189-581-7.
External links
Stains
|
|
Iron/hemosiderin |
|
|
Lipids |
- Sudan stain
- Sudan II
- Sudan III
- Sudan IV
- Oil Red O
- Sudan Black B
|
|
Carbohydrates |
- Periodic acid-Schiff stain
|
|
Amyloid |
|
|
Bacteria |
- Gram staining
- Methyl violet/Gentian violet
- Safranin
- Ziehl–Neelsen stain/acid-fast
- Carbol fuchsin/Fuchsine
- Methylene blue
- Auramine-rhodamine stain
|
|
Connective tissue |
- trichrome stain: Masson's trichrome stain/Lillie's trichrome
- Light Green SF yellowish
- Biebrich scarlet
- Phosphomolybdic acid
- Fast Green FCF
|
|
Other |
- H&E stain
- Silver stain
- Grocott's methenamine silver stain
- Warthin–Starry stain
- Methyl blue
- Wright's stain
- Giemsa stain
- Gömöri trichrome stain
- Neutral red
- Janus Green B
- Alcian blue stain
- Movat's stain
|
|
Tissue stainability |
- Acidophilic
- Basophilic
- Chromophobic
|
|
UpToDate Contents
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English Journal
- Induction of nasal polyps using house dust mite and Staphylococcal enterotoxin B in C57BL/6 mice.
- Khalmuratova R1, Lee M2, Kim DW3, Park JW2, Shin HW4.
- Allergologia et immunopathologia.Allergol Immunopathol (Madr).2015 Aug 1. pii: S0301-0546(15)00090-7. doi: 10.1016/j.aller.2015.04.004. [Epub ahead of print]
- BACKGROUND: The murine polyp model was developed previously using ovalbumin and Staphylococcus aureus enterotoxin B (SEB). Here, we established a model mimicking key aspects of chronic eosinophilic rhinosinusitis with nasal polyps using the house dust mite (HDM), a clinically relevant aeroallergen,
- PMID 26242569
- B cell autophagy mediates TLR7-dependent autoimmunity and inflammation.
- Weindel CG1, Richey LJ, Bolland S, Mehta AJ, Kearney JF, Huber BT.
- Autophagy.Autophagy.2015 Jul 3;11(7):1010-24. doi: 10.1080/15548627.2015.1052206.
- Systemic lupus erythematosus (SLE) is a heterogeneous autoimmune disease, defined by loss of B cell self-tolerance that results in production of antinuclear antibodies (ANA) and chronic inflammation. While the initiating events in lupus development are not well defined, overexpression of the RNA-rec
- PMID 26120731
Japanese Journal
- 山田 英司,片岡 和彦,藤原 俊哉,松浦 求樹
- 日本呼吸器外科学会雑誌 = The journal of the Japanese Association for Chest Surgery 26(7), 734-738, 2012-11-15
- … A Periodic-acid Schiff (PAS) stain and a PAS with diastase pre-digestion (d-PAS) stain revealed intracellular glycogen and mucin in the glands. …
- NAID 10031126001
- 宇野 友絵,南場 研一,加瀬 諭 [他],齋藤 航,北市 伸義,大野 重昭,石田 晋
- あたらしい眼科 = Journal of the eye 29(1), 135-138, 2012-01-30
- NAID 10030234641
Related Links
- PAS染色(英: Periodic acid-Schiff stain)とは組織学や病理学において使用される染色 法の一つ。PAS染色は主に組織におけるグリコーゲンの証明のために使用される。過 ヨウ素酸はグルコース残基を選択的に酸化してアルデヒドを生成し、シッフ試薬によって 赤 ...
Related Pictures
★リンクテーブル★
[★]
- 周期的な、(天)周期運動をする、周期手金あ。定期の、定時の。間欠的な、断続的な。
- 関
- cyclic, cyclical, cyclically, cyclicity, periodical, periodically, periodicity, regular, regularly, rhythmic
[★]
- 関
- coloring agent、dye、staining、tissue stain
[★]
- 関
- duration、phase、stage、term、time
[★]
- 関
- periodate