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1. 嚢胞性線維症：肺疾患治療の概要 cystic fibrosis overview of the treatment of lung disease
2. 嚢胞性線維症：栄養上の問題 cystic fibrosis nutritional issues
3. 嚢胞性線維症を有さない小児における気管支拡張症のマネージメント management of bronchiectasis in children without cystic fibrosis
4. レジオネラ感染の疫学および病因 epidemiology and pathogenesis of legionella infection
5. 膵嚢胞の分類 classification of pancreatic cysts

An efficient method to eliminate the protease activity contaminating commercial bovine pancreatic DNase I.

Le T1, Lee HJ2, Jin HJ3.
Analytical biochemistry.Anal Biochem.2015 Aug 15;483:4-6. doi: 10.1016/j.ab.2015.04.030. Epub 2015 May 2.
A method was developed to eliminate the proteases contaminating commercial DNase I, which can cause degradation of target protein during the purification process. Bio Basic DNase stock solution (in Tris-HCl buffer [pH 8.0] containing 5mM CaCl2) was first incubated at 50°C to generate autolysis of p
PMID 25944416

Children and young adults with CF in the USA have better lung function compared with the UK.

Goss CH1, MacNeill SJ2, Quinton HB3, Marshall BC4, Elbert A4, Knapp EA4, Petren K4, Gunn E5, Osmond J5, Bilton D6.
Thorax.Thorax.2015 Mar;70(3):229-36. doi: 10.1136/thoraxjnl-2014-205718. Epub 2014 Sep 25.
BACKGROUND: People with cystic fibrosis (CF) are managed differently in the USA and UK providing an opportunity to learn from differences in practice patterns.OBJECTIVES: To compare cross-sectional demographics, practice patterns and clinical outcomes between US and UK CF patients.METHODS: This was
PMID 25256255

Modified ribosome profiling reveals high abundance of ribosome protected mRNA fragments derived from 3' untranslated regions.

Miettinen TP1, Björklund M2.
Nucleic acids research.Nucleic Acids Res.2015 Jan;43(2):1019-34. doi: 10.1093/nar/gku1310. Epub 2014 Dec 29.
Ribosome profiling identifies ribosome positions on translated mRNAs. A prominent feature of published datasets is the near complete absence of ribosomes in 3' untranslated regions (3'UTR) although substantial ribosome density can be observed on non-coding RNAs. Here we perform ribosome profiling in
PMID 25550424

Molecular, biochemical and immunological studies of hen pancreatic deoxyribonuclease I

Cloning, sequencing and expression of a cDNA encoding bovine pancreatic deoxyribonuclease I in Escherichia coli : purification and characterization of the recombinant enzyme