WordNet

the chief phagocytic leukocyte; stains with either basic or acid dyes (同)neutrophile

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2015/06/01 23:40:44」(JST)

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Basophilic

Eosonophilic

Neutrophilic

A metamyelocyte is a cell undergoing granulopoiesis, derived from a myelocyte, and leading to a band cell.

It is characterized by the appearance of a bent nucleus, cytoplasmic granules, and the absence of visible nucleoli. (If the nucleus is not yet bent, then it is likely a myelocyte.)

Additional images

Hematopoiesis

External links

‹The template EMedicineDictionary is being considered for deletion.› Metamyelocyte at eMedicine Dictionary
Histology image: 01805ooa – Histology Learning System at Boston University - "Bone Marrow and Hemopoiesis: bone marrow smear, neutrophilic metamyelocyte and mature PMN"
Histology image: 75_05 at the University of Oklahoma Health Sciences Center
Histology at KUMC blood-blood11
Histology at KUMC blood-blood12
Interactive diagram at lycos.es
Slide at marist.edu
hematologyatlas.com

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1. 好中球二次顆粒欠損症 neutrophil specific granule deficiency
2. 化学療法による好中球減少症を有する成人患者と、急性白血病、骨髄異形成症候群、造血細胞移植以外の病態を有する成人患者における顆粒球コロニー刺激因子の利用 use of granulocyte colony stimulating factors in adult patients with chemotherapy induced neutropenia and conditions other than acute leukemia myelodysplastic syndrome and hematopoietic cell transplantation
3. 骨髄造血の制御 regulation of myelopoiesis
4. 白血球増多症および好中球増多症の定義およびメカニズム definition and mechanisms of leukocytosis and neutrophilia
5. 先天性好中球減少症 congenital neutropenia

English Journal

Dimethylsulfoxide exposure modulates HL-60 cell rolling interactions.

Gee DJ, Wright LK, Zimmermann J, Cole K, Soule K, Ubowski M.Author information Department of Mechanical Engineering, Rochester Institute of Technology, 76 Lomb Memorial Drive, Rochester, NY, USA. David_Gee@mail.RIT.eduAbstractHuman leukaemic HL-60 cells are widely used for studying interactions involving adhesion molecules [e.g. P-selectin and PSGL-1 (P-selectin glycoprotein ligand-1)] since their rolling behaviour has been shown to mimic the dynamics of leucocyte rolling in vitro. HL-60 cells are neutrophilic promyelocytes that can undergo granulocytic differentiation upon exposure to compounds such as DMSO (dimethylsulfoxide). Using a parallel plate flow chamber functionalized with recombinant P-selectin-Fc chimaera, undifferentiated and DMSO-induced (48, 72 and 96 h) HL-60 cells were assayed for rolling behaviour. We found that depending on P-selectin incubation concentration, undifferentiated cells incurred up to a 6-fold increase in rolling velocity while subjected to an approximately 10-fold increase in biologically relevant shear stress. HL-60 cells exposed to DMSO for up to 72 h incurred up to a 3-fold increase in rolling velocity over the same shear stress range. Significantly, cells exposed for up to 96 h incurred up to a 9-fold decrease in rolling velocity, compared with undifferentiated HL-60 cells. Although cell surface and nuclear morphological changes were evident upon exposure to DMSO, flow cytometric analysis revealed that PSGL-1 expression was unchanged, irrespective of treatment duration. The results suggest that DMSO-treated HL-60 cells may be problematic as a substitute for neutrophils for trafficking studies during advanced stages of the LAC (leucocyte adhesion cascade). We suggest that remodelling of the cell surface during differentiation may affect rolling behaviour and that DMSO-treated HL-60 cells would behave differently from the normal leucocytes during inflammatory response in vivo.
Bioscience reports.Biosci Rep.2012 Aug;32(4):375-82. doi: 10.1042/BSR20110109.
Human leukaemic HL-60 cells are widely used for studying interactions involving adhesion molecules [e.g. P-selectin and PSGL-1 (P-selectin glycoprotein ligand-1)] since their rolling behaviour has been shown to mimic the dynamics of leucocyte rolling in vitro. HL-60 cells are neutrophilic promyelocy
PMID 22494057

A novel p38-MAPK signaling axis modulates neutrophil biology in head and neck cancer.

Dumitru CA, Fechner MK, Hoffmann TK, Lang S, Brandau S.Author information Department of Otorhinolaryngology, University of Duisburg-Essen, Essen, Germany.AbstractNeutrophils are emerging as important mediators in cancer progression. Recent studies associated neutrophils with poor clinical outcome of HNC patients and showed that HNC induces recruitment, survival, and release of proinflammatory factors by neutrophils in vitro. The molecular mechanisms through which HNC and other cancers modulate neutrophil biology are currently unknown. To explore these mechanisms, we used an in vitro system that models the interaction between human HNC cells and neutrophils or neutrophilic-differentiated HL-60 cells, respectively. We show that HNC-derived factors activate p38-MAPK in neutrophils, which partly promotes neutrophil survival, but not neutrophil recruitment and motility. Most importantly, HNC-induced p38-MAPK activation strongly stimulates the release of CCL4, CXCL8, and MMP9 by neutrophils. We identify CREB and interestingly, p27 phosphorylated at T198 as downstream members of the HNC-induced p38-MAPK signaling cascade. Using siRNA technology, we demonstrate that p27 and CREB mediate the release of CCL4 and CXCL8 and that CREB, additionally, mediates the release of MMP9. These data unravel novel molecular mechanisms involved in regulation of neutrophil proinflammatory functions. Our studies on human HNC tissues indicate that tumor-infiltrating neutrophils might be a major source of CCL4 and particularly, MMP9 in cancer patients. Thus, our findings provide novel, mechanistic insights relevant for the pathophysiology of HNC and possibly, other types of cancer as well.
Journal of leukocyte biology.J Leukoc Biol.2012 Apr;91(4):591-8. doi: 10.1189/jlb.0411193. Epub 2012 Jan 18.
Neutrophils are emerging as important mediators in cancer progression. Recent studies associated neutrophils with poor clinical outcome of HNC patients and showed that HNC induces recruitment, survival, and release of proinflammatory factors by neutrophils in vitro. The molecular mechanisms through
PMID 22262799