English Journal
- [Enucleation, formation of cyto- and karyoplasts, and their fusion with neuronal body].
- Sotnikov OS, Laktionova AA, Paramonov NM, Archakova LI, Krasnova TV.AbstractIn this research that was performed on isolated neurons of mollusk Lymnaea stagnalis, using neuron enucleation, the cytoplast was obtained which was then fused with another neuron resulting in cybrid formation. The experiments performed have shown that the isolated neurons are able to fuse with each other, forming binuclear neurons; also, like all other cells, they could be enucleated with the formation of cyto- and karyoplasts and, after fusion, they can form cell body-cytoplast, cytoplasts-karyoplast, and other complexes. This is associated with the appearance of all doubtless indicators of fusion described for fusion of nerve cell bodies. This work demonstrates the possibility to artificially fuse the amputated neuroplasm fragment with neuronal cell body--the metabolic center of another cell. Theoretically, this means that in vivo amputated neuronal process also can be fused with a novel cell.
- Morfologii͡a (Saint Petersburg, Russia).Morfologiia.2012;142(4):43-8.
- In this research that was performed on isolated neurons of mollusk Lymnaea stagnalis, using neuron enucleation, the cytoplast was obtained which was then fused with another neuron resulting in cybrid formation. The experiments performed have shown that the isolated neurons are able to fuse with each
- PMID 23236890
- [Fusion of brain neurons in rat embryos].
- Sotnikov OS, Frumkina LE, Novakovskaia SA, Bogolepov NN.AbstractSyncytial interneuronal connections were studied in the sensomotor cortex and caudate nucleus of twenty 14-22 day rat embryos. It was shown that with the extremely weak development of glial processes, many neuronal bodies and their processes were in the direct contact with each other. The contacting membranes in these areas formed oblong and dot-like contacts resembling gap and tight junctions. As a result, the intercellular cleft experienced varicose-like deformations. In the area of contacts, barely visible membrane pores were formed that broadened to form large perforations. The perforation margins presented the rounded shape of fused plasma membranes of adjacent neurons. Inside the perforations, residual vesicular membranous bodies were formed. The areas of the paired membranes between perforations were fragmented, thus increasing the number of residual vesicles, until the neurons fused with each other completely by unifying the neuroplasm of contacting cells. The results of these studies suggest that that the fusion of neurons in vertebrate brain cortex and brainstem nuclei could occur not only in pathology, but also in normal animals at the stage of embryonic development.
- Morfologii͡a (Saint Petersburg, Russia).Morfologiia.2011;139(2):18-21.
- Syncytial interneuronal connections were studied in the sensomotor cortex and caudate nucleus of twenty 14-22 day rat embryos. It was shown that with the extremely weak development of glial processes, many neuronal bodies and their processes were in the direct contact with each other. The contacting
- PMID 21866800
- Neuron changes in a mollusk in response to proteolytic enzymes.
- Sotnikov OS, Lukovnikova MV, Vasyagina NY, Laktionova AA, Paramonova NM.Author information Laboratory for Neuron Functional Morphology and Physiology, Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, Russia. sotnikov@kolt.infran.ruAbstractThe aims of the present work were to investigate the structure of neurons after treatment with proteases and to identify possible recovery of interneuronal syncytial connections. In the first series of experiments, phase-contrast microscopy studies of live dissociated neurons from ganglia of the mollusk Lymnaea stagnalis treated with 0.4% pronase solution demonstrated retraction of nerve processes and biphasic changes in cell body volume. At stage I, at an average of 82.5 min, neuron body volume decreased by 12.1%, after which it increased by a mean of 14.1%. Signs of neuron viability in Ringer's solution were seen for an average of 828 min; survival time in pronase solution was 1.4 times shorter. In the second series of experiments, studies of neuron ultrastructure showed many cases of persistence of mitochondria, the rough and smooth endoplasmic reticulum (ER), Golgi complex, light and granular vesicles, nuclear structure, and neuroplasm optical density. Cells coming close together after centrifugation formed intracellular clefts of uniform width (about 20 nm). There were very rare cases of points at which membranes came into contact. There were no signs of syncytial connections. Lengthening and fusion of smooth ER cisterns separated fragments of neuron bodies from relatively undamaged cells. Some neurons were damaged, with multiple vacuoles formed form swollen mitochondria and ER cisterns. Fragments of nerve processes formed on dissociation were surrounded by a normal outer cell membrane.
- Neuroscience and behavioral physiology.Neurosci Behav Physiol.2010 Sep;40(7):773-8. doi: 10.1007/s11055-010-9325-1. Epub 2010 Jul 21.
- The aims of the present work were to investigate the structure of neurons after treatment with proteases and to identify possible recovery of interneuronal syncytial connections. In the first series of experiments, phase-contrast microscopy studies of live dissociated neurons from ganglia of the mol
- PMID 20652422
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- Neuroplasm's Upcoming Gigs Saturday 09 February, 2013.
- neuroplasm /neu·ro·plasm/ (-plazm) the protoplasm of a nerve cell.neuroplas´mic. neu·ro·plasm (n r -pl z m). n. The protoplasm of a neuron. neuroplasm [noor´o- plazm]. the protoplasm of a nerve cell. adj., adj neuroplas´mic. neuroplasm ...
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