English Journal

Development of a LacZ-based transcriptional reporter system for use with Moraxella catarrhalis.

Evans AS, Pybus C, Hansen EJ.SourceDepartment of Pediatrics, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA. Amanda.Evans@UTSouthwestern.edu
Plasmid.Plasmid.2013 Mar;69(2):180-5. doi: 10.1016/j.plasmid.2012.11.003. Epub 2012 Dec 4.
The lack of a transcriptional reporter system for use in Moraxella catarrhalis has hindered studies of gene regulation in this pathogen. PCR and recombinant DNA methods were used to insert a multicloning site (MCS) and promoterless full-length Escherichia coli lacZ gene, flanked by transcriptional t
PMID 23219721

pLR: a lentiviral backbone series to stable transduction of bicistronic genes and exchange of promoters.

Vargas JE, Salton G, Sodré de Castro Laino A, Pires TD, Bonamino M, Lenz G, Delgado-Cañedo A.SourceLaboratório de Sinalização e Plasticidade Celular, Departamento de Biofísica, Universidade Federal do Rio Grande do Sul-UFRGS, 91501-970 Porto Alegre, RS, Brazil.
Plasmid.Plasmid.2012 Nov;68(3):179-85. doi: 10.1016/j.plasmid.2012.06.001. Epub 2012 Jun 20.
Gene transfer based on lentiviral vectors allow the integration of exogenous genes into the genome of a target cell, turning these vectors into one of the most used methods for stable transgene expression in mammalian cells, in vitro and in vivo. Currently, there are no lentivectors that allow the c
PMID 22728068

Wakamori M, Umehara T, Yokoyama S.SourceRIKEN Systems and Structural Biology Center, Tsurumi, Yokohama 230-0045, Japan.
Analytical biochemistry.Anal Biochem.2012 Apr 1;423(1):184-6. doi: 10.1016/j.ab.2012.01.010. Epub 2012 Jan 26.
We developed a novel nucleosome DNA template vector, pWMD01, which is optimized for the large-scale preparation of nucleosomal DNA. By using restricted digestion by SapI or EarI within its multicloning site, multiple half-nucleosome DNA units can be introduced unidirectionally into the vector at eac
PMID 22310497

Japanese Journal

Construction of pDESTR, a GATEWAY Vector for Gene Disruption in Filamentous Fungi

ABE A
Current Microbiology 52(3), 210-215, 2006-03
… The vector was constructed by removing the multicloning site of pGEM-T easy vector, and inserting hygromycin phosphotransferase gene construct from pCB1004, and a gateway vector conversion cassette. …
NAID 80019270212

Construction and Characterization of a Bacterial Artificial Chromosome Library of the Indica Rice Kasalath

Miyazaki Chikara,Saito Akira
Breeding science 49(3), 193-201, 1999-09
… We constructed a bacterial artificial chromosome (BAC) library for the indica rice variety Kasalath using a new BAC vector, pCHR8, suited for plasmid rescue, containing nine unique restriction sites in a multicloning site. …
NAID 110001807957

Effects of Secretable SOD Delivered by Genetically Modified Cells on Xanthine/Xanthine Oxidase and Paraquat-Induced Cytotoxicity in Vitro

Komada Fusao,Nishiguchi Koushi,Tanigawara Yusuke [他],IWAKAWA Seigo,OKUMURA Katsuhiko
Biological & pharmaceutical bulletin 22(8), 846-853, 1999-08-15
… The ILSOD(2) cDNA constructed by PCR-based gane expression was ligated into the multicloning site of the pRc/CMV plasmid (pRc/CMV-ILSOD(2)). …
NAID 110003639772