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A comparative study of different protein immobilization methods for the construction of an efficient nano-structured lactate oxidase-SWCNT-biosensor.

Pagán M1, Suazo D2, Del Toro N3, Griebenow K4.
Biosensors & bioelectronics.Biosens Bioelectron.2015 Feb 15;64:138-46. doi: 10.1016/j.bios.2014.08.072. Epub 2014 Sep 3.
We constructed lactate biosensors by immobilization of lactate oxidase (LOx) onto a single-walled carbon nanotube (SWCNT) electrode. The first step of the sensor construction was the immobilization of oxidized SWCNT onto a platinum electrode modified with 4-aminothiophenol (4-ATP). Two enzyme immobi
PMID 25216450

The Ala95-to-Gly substitution in Aerococcus viridans l-lactate oxidase revisited - structural consequences at the catalytic site and effect on reactivity with O2 and other electron acceptors.

Stoisser T1, Rainer D, Leitgeb S, Wilson DK, Nidetzky B.
The FEBS journal.FEBS J.2015 Feb;282(3):562-78. doi: 10.1111/febs.13162. Epub 2014 Dec 16.
Aerococcus viridansl-lactate oxidase (avLOX) is a biotechnologically important flavoenzyme that catalyzes the conversion of L-lactate and O₂ into pyruvate and H₂O₂. The enzymatic reaction underlies different biosensor applications of avLOX for blood L-lactate determination. The ability of avLO
PMID 25423902

3D graphene nano-grid as a homogeneous protein distributor for ultrasensitive biosensors.

Chu Z1, Shi L1, Jin W2.
Biosensors & bioelectronics.Biosens Bioelectron.2014 Nov 15;61:422-8. doi: 10.1016/j.bios.2014.05.006. Epub 2014 Jun 4.
In order to realize the protein uniform immobilization, a 3D nano-gird architecture of thiol grafted graphene film was fabricated to serve as a novel linker between protein and substrate. Relied on the online monitor by QCM, graphene deposition process can be exactly controlled to construct the perf
PMID 24934742

Ascorbic acid attenuates acute pulmonary oxidative stress and inflammation caused by zinc oxide nanoparticles

Ascorbic acid attenuates acute pulmonary oxidative stress and inflammation caused by zinc oxide nanoparticles

Cytoprotective Effect of Alpha-Lipoic Acid on Paraquat-Exposed Human Bronchial Epithelial Cells <i>via</i> Activation of Nuclear Factor Erythroid Related Factor-2 Pathway