イソ制限酵素、アイソシゾマー
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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2016/11/11 19:54:30」(JST)
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Isoschizomers are pairs of restriction enzymes specific to the same recognition sequence. For example, SphI (CGTAC/G) and BbuI (CGTAC/G) are isoschizomers of each other. The first enzyme discovered which recognizes a given sequence is known as the prototype; all subsequently identified enzymes that recognize that sequence are isoschizomers. Isoschizomers are isolated from different strains of bacteria and therefore may require different reaction conditions.
An enzyme that recognizes the same sequence but cuts it differently is a neoschizomer. Neoschizomers are a specific type (subset) of isoschizomer. For example, SmaI (CCC/GGG) and XmaI (C/CCGGG) are neoschizomers of each other. Similarly Kpn1 (GGTAC/C) and Acc651 (G/GTACC) are neoschizomers of each other. An enzyme that recognizes a slightly different sequence, but produces the same ends is an isocaudomer.
In some cases, only one out of a pair of isoschizomers can recognize both the methylated as well as unmethylated forms of restriction sites. In contrast, the other restriction enzyme can recognize only the unmethylated form of the restriction site. This property of some isoschizomers allows identification of methylation state of the restriction site while isolating it from a bacterial strain. For example, the restriction enzymes HpaII and MspI are isoschizomers, as they both recognize the sequence 5'-CCGG-3' when it is unmethylated. But when the second C of the sequence is methylated, only MspI can recognize it while HpaII cannot.
References
"Isoschizomers at New England Biolabs".
English Journal
- TfoI produced by Tepidimonas fonticaldi PL17, a moderate thermophilic bacterium, is an isoschizomer of MseI.
- Kumar R1, Pinnaka AK1, Krishnan B2.
- Extremophiles : life under extreme conditions.Extremophiles.2017 May;21(3):523-535. doi: 10.1007/s00792-017-0922-6. Epub 2017 Mar 18.
- PMID 28315976
- Perpetuating the homing endonuclease life cycle: identification of mutations that modulate and change I-TevI cleavage preference.
- Roy AC1, Wilson GG2, Edgell DR3.
- Nucleic acids research.Nucleic Acids Res.2016 Sep 6;44(15):7350-9. doi: 10.1093/nar/gkw614. Epub 2016 Jul 7.
- PMID 27387281
- A putative Type IIS restriction endonuclease GeoICI from Geobacillus sp.--A robust, thermostable alternative to mezophilic prototype BbvI.
- Zebrowska J1, Zolnierkiewicz O, Skowron MA, Zylicz-Stachula A, Jezewska-Frackowiak J, Skowron PM.
- Journal of biosciences.J Biosci.2016 Mar;41(1):27-38.
- PMID 26949085
Japanese Journal
- Analysis of Developmental Changes in Avian DNA Methylation Using a Novel Method for Quantifying Genome-wide DNA Methylation
- Genome-wide profiling of promoter methylation in human
- Comparative isoschizomer profiling of cytosine methylation : the HELP assay
Related Links
- Isoschizomer一覧表 Isoschizomer一覧表 カタログの見方 * R ： A or G K ： G or T H ： A or C or T Y ： C or T M ： A or C V ： A or C or G W ： A or T B ： C or G or T N ： A or C or G or T S ： G or C D ： A or G or T ...
- i·so·schiz·o·mer (ī'sō-skiz'ō-mĕr), A restriction endonuclease from different organisms that recognizes and hydrolyzes at the same DNA sequence. [jiso- + G. schizō to split, + -mer] isoschizomer a RESTRICTION ENZYME that ...
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