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1. 成人における皮膚筋炎および多発性筋炎の初期治療 initial treatment of dermatomyositis and polymyositis in adults
2. 薬理ゲノム学の概要 overview of pharmacogenomics
3. 6-メルカプトプリン（6-MP）またはアザチオプリンを用いた炎症性腸疾患治療における6-MP代謝産物モニタリングおよびTPMT検査 6 mercaptopurine 6 mp metabolite monitoring and tpmt testing in the treatment of inflammatory bowel disease with 6 mp or azathioprine
4. 成人における皮膚筋炎および多発性筋炎の臨床症状 clinical manifestations of dermatomyositis and polymyositis in adults
5. 炎症性ミオパチーの病因 pathogenesis of inflammatory myopathies

English Journal

ICESluvan, a 94-Kilobase Mosaic Integrative Conjugative Element Conferring Interspecies Transfer of VanB-Type Glycopeptide Resistance, a Novel Bacitracin Resistance Locus, and a Toxin-Antitoxin Stabilization System.

Bjørkeng EK, Hjerde E, Pedersen T, Sundsfjord A, Hegstad K.SourceResearch Group for Host-Microbe Interactions, Department of Medical Biology, Faculty of Health Sciences, University of Tromsø, Tromsø, Norway.
Journal of bacteriology.J Bacteriol.2013 Dec;195(23):5381-90. doi: 10.1128/JB.02165-12. Epub 2013 Sep 27.
A 94-kb integrative conjugative element (ICESluvan) transferable to Enterococcus faecium and Enterococcus faecalis from an animal isolate of Streptococcus lutetiensis consists of a mosaic of genetic fragments from different Gram-positive bacteria. A variant of ICESluvan was confirmed in S. lutetiens
PMID 24078615

Altered 2-thiouridylation impairs mitochondrial translation in reversible infantile respiratory chain deficiency.

Boczonadi V, Smith PM, Pyle A, Gomez-Duran A, Schara U, Tulinius M, Chinnery PF, Horvath R.SourceInstitute of Genetic Medicine, Newcastle University, Central Parkway, Newcastle upon Tyne NE1 3BZ, UK.
Human molecular genetics.Hum Mol Genet.2013 Nov 15;22(22):4602-15. doi: 10.1093/hmg/ddt309. Epub 2013 Jun 28.
Childhood-onset mitochondrial encephalomyopathies are severe, relentlessly progressive conditions. However, reversible infantile respiratory chain deficiency (RIRCD), due to a homoplasmic mt-tRNA(Glu) mutation, and reversible infantile hepatopathy, due to tRNA 5-methylaminomethyl-2-thiouridylate met
PMID 23814040

Activation of a Unique Flavin-Dependent tRNA Methylating Agent.

Hamdane D, Bruch EM, Un S, Field MJ, Fontecave M.AbstractTrmFO is a tRNA methyltransferase using methylenetetrahydrofolate (CH2THF) and a flavin adenine dinucleotide hydroquinone as cofactors. We have recently shown that TrmFO from Bacillus subtilis stabilizes a TrmFO-CH2-FADH adduct and an ill-defined neutral flavin radical. The adduct contains a unique N-CH2-S moiety, with a methylene group bridging N5 of the isoalloxazine ring and the sulfur of an active site cysteine (Cys53). In the absence of tRNA substrate, this species is remarkably stable but becomes catalytically competent, using the methylene group as the source of methyl, for tRNA methylation following tRNA addition. Here we demonstrate that this dormant methylating agent can be activated at low pH and propose that this process is triggered upon tRNA addition. The reaction proceeds via protonation of Cys53, cleavage of the C-S bond and generation of a highly reactive [FADH(N5)= CH2]+ iminium intermediate, proposed as the actual tRNA methylating agent. This mechanism is fully supported by DFT calculations. The radical present in TrmFO is characterized here by optical and EPR/ENDOR spectroscopies together with DFT calculations and shown to be the one-electron oxidized product of the TrmFO-CH2-FADH adduct. It is also relatively stable and its decomposition is facilitated at high pH. These results provide new insights into the structure and reactivity of the unique flavin-dependent methylating agent used by this class of enzymes.
Biochemistry.Biochemistry.2013 Nov 14. [Epub ahead of print]
TrmFO is a tRNA methyltransferase using methylenetetrahydrofolate (CH2THF) and a flavin adenine dinucleotide hydroquinone as cofactors. We have recently shown that TrmFO from Bacillus subtilis stabilizes a TrmFO-CH2-FADH adduct and an ill-defined neutral flavin radical. The adduct contains a unique
PMID 24228791

Japanese Journal

Trmt112 Gene Expression in Mouse Embryonic Development

Gu Tiantian,He Hongjuan,Zhang Yan,Han Zhengbin,Hou Guangyuan,Zeng Tiebo,Liu Qi,Wu Qiong
ACTA HISTOCHEMICA ET CYTOCHEMICA 45(2), 113-119, 2012
… Mouse Trmt112, the homologous gene of yeast Trm112 (tRNA methyltransferase 11-2), was initially cloned from RIKEN with uncertain function. …
NAID 130001854238

3B1322 細菌型tRNA37位グアノシンm^1メチル基転移酵素と基質tRNA、メチル基供与体アナログの複合体の結晶構造(3B 核酸結合蛋白質,日本生物物理学会第49回年会)

Ito Takuhiro,Yoshida Kenichi,Goto-Ito Sakurako,Yokoyama Shigeyuki
生物物理 51(SUPPLEMENT_1), S110-S111, 2011-08-15
NAID 110008903397

トランスファーRNAメチル化酵素の分子進化

堀弘幸
工学ジャーナル 4, 95-104, 2004-03
… これほど多様に細分化されたRNAメチル化酵素群を包括的にとらえ、その概念を統一することは可能であろうか?また、その分子進化を追跡できないであろうか?このような観点から、我々はtRNAメチル化酵素をモデルとして構造・機能解析を進めてきた。 … RNA修飾ヌクレオシドはtRNA中にもっとも多く、タンパク質合成系を円滑かつ正確に動作させるために必須の因子である。 …
NAID 110004708226

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★リンクテーブル★

リンク元	「tRNAメチル基転移酵素」「tRNAメチルトランスフェラーゼ」
関連記事	「methyltransferase」「tRNA」「tRNAs」「methyltransferases」