関: mengo virus

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2016/03/16 00:29:54」(JST)

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Mengovirus, also known as Columbia SK virus, mouse Elberfield virus and Encephalomyocarditisvirus (EMCV), belongs to the genus Cardiovirus which is a member of the Picornaviridae. Its genome is a single stranded positive-sense RNA molecule, making the Mengoviruses a class IV virus under the Baltimore classification system. The genome is approximately 8400nt in length, and has 5’ VG protein (Virus genome protein) and a 3’ polyadenine tail.

Structure

The Mengovirus is a non-enveloped virus which has a nucleocapsid made up of 12 subunits. The virion is 30 nm in diameter and displays icosohedral symmetry.

Gene Expression and Genome Replication

Once inside a host cell, the Mengovirus genome acts as a piece of mRNA, and is directly translated by host ribosomes in the cytoplasm. There is a large un-translated region at the 5’ end of the RNA that has a ribosome binding site, removing the need of a cap. A single polypeptide is made, and is cleaved into individual proteins by viral proteases. The genome is divided into three parts: P1, P2 and P3. P1 encodes the virus capsid proteins, P2 and P3 encode genes required for genome replication to occur. For replication to occur an intermediate double stranded RNA molecule is made to be used as a template for the production of positive sense genomes.

Infection

Mengovirus is infectious to vertebrate animals, and has been isolated from mice and other rodents. It can also cause acute fever in humans. There is no specific treatment for a Mengovirus infection; although Persantine has been shown to inhibit its replication.^[1] The illness is not severe enough to require vaccination. The Mengovirus is able to suppress the host’s immune response by reducing the expression of Nuclear Factor kappa B using the 5’ un-translated region.

References

^ http://jvi.asm.org/cgi/content/full/79/17/11062?view=long&pmid=16103157

English Journal

Standardized multiplex one-step qRT-PCR for hepatitis A virus, norovirus GI and GII quantification in bivalve mollusks and water.

Fuentes C1, Guix S1, Pérez-Rodriguez FJ1, Fuster N1, Carol M2, Pintó RM3, Bosch A4.
Food microbiology.Food Microbiol.2014 Jun;40:55-63. doi: 10.1016/j.fm.2013.12.003. Epub 2013 Dec 30.
A quadruplex Real-Time RT-PCR assay for the simultaneous quantitative detection of hepatitis A virus (HAV), norovirus (NoV) GI and GII, and mengovirus (used as process control for determination of the virus/nucleic acid extraction efficiency) has been developed. This multiplex assay has been compara
PMID 24549198

Reduction of cell viability induced by IFN-alpha generates impaired data on antiviral assay using Hep-2C cells.

de Oliveira ER1, Lima BM, de Moura WC, Nogueira AC.
Journal of immunological methods.J Immunol Methods.2013 Dec 31;400-401:97-105. doi: 10.1016/j.jim.2013.10.011. Epub 2013 Nov 7.
Type I interferons (IFNs) exert an array of important biological functions on the innate immune response and has become a useful tool in the treatment of various diseases. An increasing demand in the usage of recombinant IFNs, mainly due to the treatment of chronic hepatitis C infection, augmented t
PMID 24211646

Preliminary Study to Assess the Performance of Mengovirus Elution from Sludge.

Amdiouni H1, Soukri A, Nourlil J, Maunula L.
Food and environmental virology.Food Environ Virol.2013 Jun 25. [Epub ahead of print]
In the virus detection protocol for sludge, the viral elution step from solids to solution is critical. In this study, mengoviruses were detected in artificially contaminated sludge with a qRT-PCR assay. The viral yields ranged between 19 and 66 % for 60 % sludge. This study demonstrates that meng
PMID 23797496

Japanese Journal

The mengovirus leader protein suppresses alpha/beta interferon production by inhibition of the iron/ferritin-mediated activation of NF-kappa B

ZOLL J
J Virol 76, 9664-9672, 2002
NAID 30020796995

THE GROWTH KINETICS OF AN ATTENUATED POLIOVIRUS TYPE 1 STRAIN, LSc, 2ab AT AN ELEVATED TEMPERATURE

山崎善市,戸塚敦子,大滝研也
Japanese Journal of Medical Science and Biology 23(6), 381-393, 1970
… (1963) : A new ribonucleic acid polymerase appearing after mengovirus infection of L-cells. …
NAID 130003778246