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Enzyme-Immobilized 3D-Printed Reactors for Online Monitoring of Rat Brain Extracellular Glucose and Lactate.

Su CK1, Yen SC2, Li TW2, Sun YC2.
Analytical chemistry.Anal Chem.2016 Jun 21;88(12):6265-73. doi: 10.1021/acs.analchem.6b00272. Epub 2016 May 27.
In this study we constructed a highly sensitive system for in vivo monitoring of the concentrations of rat brain extracellular glucose and lactate. This system involved microdialysis (MD) sampling and fluorescence determination in conjunction with a novel sample derivatization scheme in which glucos
PMID 27232384

Conformational flexibility related to enzyme activity: evidence for a dynamic active-site gatekeeper function of Tyr(215) in Aerococcus viridans lactate oxidase.

Stoisser T1,2, Brunsteiner M1, Wilson DK3, Nidetzky B1,2.
Scientific reports.Sci Rep.2016 Jun 15;6:27892. doi: 10.1038/srep27892.
L-Lactate oxidase (LOX) belongs to a large family of flavoenzymes that catalyze oxidation of α-hydroxy acids. How in these enzymes the protein structure controls reactivity presents an important but elusive problem. LOX contains a prominent tyrosine in the substrate binding pocket (Tyr(215) in Aero
PMID 27302031

Electrocatalytic processes promoted by diamond nanoparticles in enzymatic biosensing devices.

Briones M1, Petit-Domínguez MD1, Parra-Alfambra AM1, Vázquez L2, Pariente F1, Lorenzo E3, Casero E4.
Bioelectrochemistry (Amsterdam, Netherlands).Bioelectrochemistry.2016 May 26;111:93-99. doi: 10.1016/j.bioelechem.2016.05.007. [Epub ahead of print]
We have developed a biosensing platform for lactate determination based on gold electrodes modified with diamond nanoparticles of 4nm of nominal diameter, employing the enzyme lactate oxidase and (hydroxymethyl)ferrocene (HMF) as redox mediator in solution. This system displays a response towards la
PMID 27261566