乳酸脱水素酵素ウイルス

WordNet

a harmful or corrupting agency; "bigotry is a virus that must not be allowed to spread"; "the virus of jealousy is latent in everyone"
(virology) ultramicroscopic infectious agent that replicates itself only within cells of living hosts; many are pathogenic; a piece of nucleic acid (DNA or RNA) wrapped in a thin coat of protein
a software program capable of reproducing itself and usually capable of causing great harm to files or other programs on the same computer; "a true virus cannot spread to another computer without human assistance" (同)computer virus
a salt or ester of lactic acid

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2015/12/11 08:13:55」(JST)

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Lactate dehydrogenase elevating virus, or LDV for short, belongs to part of the arteriviridae family and the nidovirales order. Also included in the nidovirales order are the coronaviridae. The arteriviridae infect macrophages in animals and cause a variety of diseases.^[1] LDV specifically causes lifelong persistent viremia in mice, but doesn't really harm the host and only slightly harms the immune system. The main clinical sign is an increased level of the plasma enzyme lactate dehydrogenase (LDH). LDV has a remarkably narrow cell type specificity, meaning nothing homologous with LDV in mice has been found in another species.^[2]

Discovery

LDV was discovered in 1960 by Dr. Vernon Riley and his colleagues while they were working with plasma enzymes in tumor-bearing mice. They found that many types of transplantable tumors caused a five to tenfold increase in the plasma lactate dehydrogenase (LDH) activity within three days of the transplantation. This occurred even before the tumors were obvious clinically. In further investigations, they found that cell-free plasma from tumor-bearing mice was sufficient to cause this increase, which indicated that the agent was small, and further investigation showed that it was a virus.^[1]

Transmission

Because the virus causes persistent viremia, virus in the bloodstream, it can be spread by blood-sucking ectoparasites. This is the theory for spread of virus in feral or wild mice which have been found to be infected in Europe, America, and Australia. Another method of transmission is from the mother to her fetus during pregnancy; however, this mode is much less likely in a chronically infected mother mouse. The mother can also spread the virus to her young through her milk.^[3] Studies with male mice have shown that they seldom transmit the virus; however, when an infected male fathers a litter with an uninfected female, there are more females in the litter than would be expected, and none of them are infected. However, this is controversial; some believe that it can be spread thought sexual contact. Other modes include fighting and cannibalism.^[1]

Structure

LDV has a genome that consists of single stranded positive sense RNA that is 14.1kb long. The genome is dominated by two large open reading frames, ORF1a and ORF1ab; these code for two polyproteins, PP1a and PP1ab. These polyproteins are thought to be cleaved into 12 products. The virus contains a nucleocapsid that is spherical with a diameter of 35 ± 4 nm. This is then enclosed in an envelope to create a smooth surface. The envelope consists of two proteins, VP2 and VP3. VP2 has a molecular weight of 18,000, and VP3 is a heterogeneous glycoprotein of molecular weight 15,000.^[4] The envelope is extremely labile and tends to slough off; this characteristic is indicated by its extreme sensitivity to detergent treatment. The virus has a density of 1.13g/mL and the nucleocapsid has a density of 1.17g/mL in a sucrose density gradient.^[5] LDV has been shown to mature by budding through the intracytoplasmic membrane. The virions have four structural proteins which include a nucleocapsid protein, a non-glycosylated envelope protein, a major envelope glycoprotein, and a minor envelope glycoprotein.^[4]

Pathogenesis

As mentioned before, LDV has a very high specificity. Studies have shown that LDV is not only host specific, but cell specific as well. The first cells it was shown to replicate in were primary mouse embryo cell cultures, but these cultures had to be freshly explanted. After about seven days the cells lost their ability to support LDV even though they could support other viruses. When other mouse tissues were tested it was found that peritoneal macrophages consistently yielded the highest virus titers. Further studies have shown that in the first twenty-four hours after infection of a macrophage there is very rapid virus replication; this rate gradually falls off to a very low level but continues as long as the macrophage continues to divide. The viremia arises because LDV lyses the cell after replication. The virus is most commonly found in the liver, spleen, lymph nodes, and skin. The main effect of the virus on the host cell is to increase the activity of certain plasma enzymes; this increase in activity is not directly related to the level of viral infectivity, but does depend on the balance between rate of entry and rate of clearance and evidence leans more heavily towards the rate of clearance. In the plasma, LDH consists of five isoenzymes and LDV generally only has an effect on LDH A4. Another effect only occurs in the C58 and AKR type mice and involves destruction of lower motor neurons producing age-dependent polioencephalomyelitis. Other effects of the virus include a temporary fall in the total white blood cell count that lasts for twenty-four hours after infection.^[4]

References

^ ^a ^b ^c Rowson, K.E.K.; B.W.J. Mahy (1985). "Lactate Dehydrogenase-Elevating Virus". Journal of General Virology. pp. 2297–2312.
^ Mohammadi, Hakimeh; Shayan Sharif; Raymond R. Rowland; Dongwan Yon (11 June 2009). "The Lactate Dehydrogenase-Elevating Virus Capsid Protein is a Nuclear-Cytoplasmic Protein". Archives of Virology. pp. 1071–1080.
^ "Lactate Dehydrogenase Elevating Virus" (PDF). Charles River Technical Sheet.
^ ^a ^b ^c Anderson, Grant W.; Raymond R. R. Rowland; Gene A. Palmer; Chen Even; Peter G. W. Plagemann (August 1995). "Lactate Dehydrogenase-Elevating Virus Replication Persists in Liver, Spleen, Lymph Node, and Testis Tissues and Results in Accumulation of Viral RNA in Germinal Centers, Concomitant with Polycolonal Activation of B Cells.". J. Virol. (Journal of Virology) 69 (8): 5177–5185. PMC 189342. PMID 7609091.
^ Brinton-Darnell, Margo; Peter G. W. Plagemann (August 1975). "Structure and Chemical-Physical Characteristics of Lactate Dehydrogenase-Elevating Virus and its RNA". J. Virol. (Journal of Virology) 16 (2): 420–433. PMC 354680. PMID 1171266.

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English Journal

Modulation of innate immune signaling by nonstructural protein 1 (nsp1) in the family Arteriviridae.

Han M1, Yoo D2.
Virus research.Virus Res.2014 Dec 19;194:100-9. doi: 10.1016/j.virusres.2014.09.007. Epub 2014 Sep 28.
Arteriviruses infect immune cells and may cause persistence in infected hosts. Inefficient induction of pro-inflammatory cytokines and type I IFNs are observed during infection of this group of viruses, suggesting that they may have evolved to escape the host immune surveillance for efficient surviv
PMID 25262851

Modulation of the host microenvironment by a common non-oncolytic mouse virus leads to inhibition of plasmacytoma development through NK cell activation.

Thirion G1, Saxena A1, Hulhoven X1, Markine-Goriaynoff D1, Van Snick J2, Coutelier JP3.
The Journal of general virology.J Gen Virol.2014 Jul;95(Pt 7):1504-9. doi: 10.1099/vir.0.063990-0. Epub 2014 Apr 16.
Although many cells undergo transformation, few actually develop into tumours, due to successful mechanisms of immunosurveillance. To investigate whether an infectious agent may play a role in this process, the growth of a plasmacytoma was investigated in mice infected by lactate dehydrogenase-eleva
PMID 24739273

Biogenesis of non-structural protein 1 (nsp1) and nsp1-mediated type I interferon modulation in arteriviruses.

Han M1, Kim CY1, Rowland RR2, Fang Y2, Kim D1, Yoo D3.
Virology.Virology.2014 Jun;458-459:136-50. doi: 10.1016/j.virol.2014.04.028. Epub 2014 May 13.
Type I interferons (IFNs-α/β) play a key role for the antiviral state of host, and the porcine arterivirus; porcine reproductive and respiratory syndrome virus (PRRSV), has been shown to down-regulate the production of IFNs during infection. Non-structural protein (nsp) 1 of PRRSV has been identif
PMID 24928046

Japanese Journal

Divergent roles of IFNs in the sensitization to endotoxin shock by lactate dehydrogenase-elevating virus

LE-THI-PHUONG Thao,DUMOUTIER Laure,RENAULD Jean-Christophe,VAN SNICK Jacques,COUTELIER Jean-Paul
International immunology 19(11), 1303-1311, 2007-11-01
NAID 10020107442

Distinct gamma interferon-production pathways in mics infected with lactate dehydrogenase-elevating virus

LE-THI-PHUONG T.
J. Gen. Virol. 88, 3063, 2007
NAID 30030030619

The murine antibody response to lactate dehydrogenase-elevating virus

COUTELIER J. -P.
J. Gen. Virol. 67, 1099, 1986
NAID 30037646828

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リンク元	「乳酸脱水素酵素ウイルス」
関連記事	「lactating」「dehydrogenase」「dehydrogenases」「lactate dehydrogenase」「virus」

「乳酸脱水素酵素ウイルス」

Lactate dehydrogenase elevating virus
Virus classification
Group:	Group IV ((+)ssRNA)
Order:	Nidovirales
Family:	Arteriviridae
Genus:	Arterivirus
Species:	Lactate dehydrogenase elevating virus