English Journal

Dansylation metabolite assay: a simple and rapid method for sample amount normalization in metabolomics.

Wu Y1, Li L.
Analytical chemistry.Anal Chem.2014 Oct 7;86(19):9428-33. doi: 10.1021/ac503359v. Epub 2014 Sep 29.
Metabolomics involves the comparison of the metabolomes of individual samples from two or more groups to reveal the metabolic differences. In order to measure the metabolite concentration differences accurately, using the same amount of starting materials is essential. In this work, we describe a si
PMID 25215550

Development of isotope labeling liquid chromatography mass spectrometry for mouse urine metabolomics: quantitative metabolomic study of transgenic mice related to Alzheimer's disease.

Peng J1, Guo K, Xia J, Zhou J, Yang J, Westaway D, Wishart DS, Li L.
Journal of proteome research.J Proteome Res.2014 Oct 3;13(10):4457-69. doi: 10.1021/pr500828v. Epub 2014 Sep 15.
Because of a limited volume of urine that can be collected from a mouse, it is very difficult to apply the common strategy of using multiple analytical techniques to analyze the metabolites to increase the metabolome coverage for mouse urine metabolomics. We report an enabling method based on differ
PMID 25164377

Quantitation of glutathione and its oxidation products in erythrocytes by multiple-label stable-isotope dilution.

Reinbold J1, Koehler P, Rychlik M.
Analytical biochemistry.Anal Biochem.2014 Jan 15;445:41-8. doi: 10.1016/j.ab.2013.09.029. Epub 2013 Oct 10.
A multiple-label stable isotope dilution assay for quantifying glutathione (GSH), glutathione disulfide (GSSG), and glutathione sulfonic acid in erythrocytes was developed. As the internal standards, [(13)C3,(15)N]glutathione, [(13)C4,(15)N2]glutathione disulfide, and [(13)C3,(15)N]glutathione sulfo
PMID 24120409

Japanese Journal

Determination of muscimol and ibotenic acid in Amanita mushrooms by high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry

TSUJIKAWA Kenji,KUWAYAMA Kenji,MIYAGUCHI Hajime,KANAMORI Tatsuyuki,IWATA Yuko,INOUE Hiroyuki,YOSHIDA Takemi,KISHI Tohru
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 852(1), 430-435, 2007-06-01
NAID 10025802505

Dansylation of Endocrine-disrupting Alkylphenolic Compounds on the Solid Phase Used for Extraction from Environmental Water

An Improved Simultaneous Measurement of Oxidized and Reduced Glutathione in Biological Samples by High-Performance Liquid Chromatography Following Derivatization with Dansyl Chloride

Nishijima Hironori,Yoshida Minoru,Takeya Akira [他],Hara Masayuki,Sagi Morihisa,Sakata Noriaki,Mukai Toshiji,Yamazaki Kentaro
Journal of health science 45(6), 324-328, 1999-12-31
… In the present study, two points were investigated in detail to improve upon their method : 1) pH in the derivatization reaction and 2) the method of deproteinization prior to dansylation. … Dansylation of standard GSH after S-carboxymethylation and of GSSG both gave the highest relative fluorescence at pH 8.5.Degradation of GSSG to GSH was not detected when GSSG was dansylated at pH 8.5 or more acidic pHs but was apparent at pH 9.0 and even more obvious at pH 10.0. …
NAID 110003641579