English Journal

High throughput cryopreservation of cells by rapid freezing of sub-μl drops using inkjet printing - cryoprinting.

Dou R1, Saunders RE, Mohamet L, Ward CM, Derby B.
Lab on a chip.Lab Chip.2015 Sep 7;15(17):3503-13. doi: 10.1039/c5lc00674k. Epub 2015 Jul 20.
We have successfully used inkjet printing to cryopreserve 3T3 mouse fibroblast cells and human neuroprogenitor cells (NPCs) derived from human embryonic stem cells (hESCs). Sessile drops of volume 114 nl were formed by printing cell suspensions containing dimethyl sulphoxide (DMSO) as a cryoprotecti
PMID 26190571

Correlating steric hydration forces with water dynamics through surface force and diffusion NMR measurements in a lipid-DMSO-H2O system.

Schrader AM1, Donaldson SH Jr1, Song J2, Cheng CY2, Lee DW1, Han S3, Israelachvili JN4.
Proceedings of the National Academy of Sciences of the United States of America.Proc Natl Acad Sci U S A.2015 Aug 10. pii: 201512325. [Epub ahead of print]
Dimethyl sulfoxide (DMSO) is a common solvent and biological additive possessing well-known utility in cellular cryoprotection and lipid membrane permeabilization, but the governing mechanisms at membrane interfaces remain poorly understood. Many studies have focused on DMSO-lipid interactions and t
PMID 26261313

The MORPHEUS II protein crystallization screen.

Gorrec F1.
Acta crystallographica. Section F, Structural biology communications.Acta Crystallogr F Struct Biol Commun.2015 Jul;71(Pt 7):831-7. doi: 10.1107/S2053230X1500967X. Epub 2015 Jun 27.
High-quality macromolecular crystals are a prerequisite for the process of protein structure determination by X-ray diffraction. Unfortunately, the relative yield of diffraction-quality crystals from crystallization experiments is often very low. In this context, innovative crystallization screen fo
PMID 26144227

Japanese Journal

Toward a Molecular Understanding of the Mechanism of Cryopreservation by Polyampholytes: Cell Membrane Interactions and Hydrophobicity

Biomacromolecules 17(5), 1882-1893, 2016-04-14
NAID 120005820918

両性電解質による細胞凍結保護効果(セミナー「NIAS International Seminar for Cryobiology and Cryotechnology」)

低温生物工学会誌 59(1), 23-28, 2013-04-15
NAID 110009612763

凍結保護活性を持つカルボキシル化ポリリジン溶液の固体NMR測定による凍結時の挙動の評価

低温生物工学会誌 57(2), 131-134, 2011-10-15
NAID 110009457795

Related Links

cryoprotection - definition of cryoprotection by Medical dictionary

After complete perfusion the brains were removed and post-fixed for 4 h at room temperature in plastic containers filled with the same fixative solution; for cryoprotection the fixed brains were immersed in 15% sucrose (in phosphate ...