English Journal

Reshaping of T-lymphocyte compartment in adult prepubertaly ovariectomised rats: A putative role for progesterone deficiency.

Leposavić G1, Nanut MP2, Pilipović I2, Kosec D2, Arsenović-Ranin N3, Stojić-Vukanić Z3, Djikić J4, Nacka-Aleksić M4.Author information 1Department of Physiology, University of Belgrade, Faculty of Pharmacy, 450 Vojvode Stepe, 11221 Belgrade, Serbia. Electronic address: Gordana.Leposavic@pharmacy.bg.ac.rs.2Immunology Research Centre "Branislav Janković", Institute of Virology, Vaccines and Sera, "Torlak", 458 Vojvode Stepe, 11221 Belgrade, Serbia.3Department of Microbiology and Immunology, University of Belgrade, Faculty of Pharmacy, 450 Vojvode Stepe, 11221 Belgrade, Serbia.4Department of Physiology, University of Belgrade, Faculty of Pharmacy, 450 Vojvode Stepe, 11221 Belgrade, Serbia.AbstractThis study explores the role of ovarian hormones in the phenotypic shaping of peripheral T-cell pool over the reproductive lifespan of rats. For this purpose, 2-month-old prepubertally ovariectomised (Ox) rats, showing oestrogen and progesterone deficiency, and 11-month-old Ox rats, exhibiting only progesterone deficiency, were examined for thymus output, and cellularity and composition of major TCRαβ+ peripheral blood lymphocyte (PBL) and splenocyte subsets. Although ovariectomy increased thymic output in both 2- and 11-month-old rats, the count of both CD4+ and CD8+ PBLs and splenocytes increased only in the former. In the blood and spleen of 11-month-old Ox rats only the count of CD8+ cells increased. Although ovariectomy affected the total CD4+ count in none of the examined compartments from the 11-month-old rats, it increased CD4+FoxP3+ PBL and splenocyte relative proportions over those in the age-matched controls. The age-related differences in the cellularity and the major subset composition in Ox rats were linked to the differences in the ovarian steroid hormone levels registered in 2- and 11-month-old rats. The administration of progesterone to Ox rats during the seven days before the sacrificing confirmed contribution of this hormone deficiency to the ovariectomy-induced changes in the TCRαβ+ PBL and splenocyte pool from 11-month-old rats. The expansion of the CD8+ splenocyte subset in the 11-month-old Ox rats reflected increases in cellularity of memory and, particularly, naïve cells. This was due to greater thymic output of CD8+ cells and homeostatic proliferation than apoptosis in 11-month-old Ox rats when compared with age-matched sham-Ox control rats. The homeostatic changes within CD8+ splenocyte pool from 11-month-old Ox rats, most likely, reflected the enhanced splenic IL-7 and TGF-β mRNA expression. Overall, in adult female rats, circulating oestrogen and progesterone provide maintenance of T-cell counts, a diversity of T-cell repertoire, and the main T-cell subset composition in the periphery. Progesterone deficiency affects mainly the CD8+ lymphocyte compartment through increasing thymic CD8+ cell export and upsetting homeostatic regulation within the CD8+ splenocyte pool. These alterations were reversible through progesterone supplementation.
Immunobiology.Immunobiology.2014 Feb;219(2):118-30. doi: 10.1016/j.imbio.2013.08.004. Epub 2013 Aug 23.
This study explores the role of ovarian hormones in the phenotypic shaping of peripheral T-cell pool over the reproductive lifespan of rats. For this purpose, 2-month-old prepubertally ovariectomised (Ox) rats, showing oestrogen and progesterone deficiency, and 11-month-old Ox rats, exhibiting only
PMID 24054944

Improved circulating microparticle analysis in acid-citrate dextrose (ACD) anticoagulant tube.

György B1, Pálóczi K2, Kovács A2, Barabás E3, Bekő G3, Várnai K3, Pállinger E2, Szabó-Taylor K2, Szabó TG2, Kiss AA4, Falus A2, Buzás EI5.Author information 1Semmelweis University, Department of Genetics, Cell- and Immunobiology, Budapest, Hungary. Electronic address: gyorgyben@gmail.com.2Semmelweis University, Department of Genetics, Cell- and Immunobiology, Budapest, Hungary.3Semmelweis University, Department of Laboratory Medicine, Budapest, Hungary.4Military Hospital, National Health Institute, Department of Obstetrics and Gynecology, Budapest, Hungary.5Semmelweis University, Department of Genetics, Cell- and Immunobiology, Budapest, Hungary. Electronic address: edit.buzas@gmail.com.AbstractINTRODUCTION: Recently extracellular vesicles (exosomes, microparticles also referred to as microvesicles and apoptotic bodies) have attracted substantial interest as potential biomarkers and therapeutic vehicles. However, analysis of microparticles in biological fluids is confounded by many factors such as the activation of cells in the blood collection tube that leads to in vitro vesiculation. In this study we aimed at identifying an anticoagulant that prevents in vitro vesiculation in blood plasma samples.
Thrombosis research.Thromb Res.2014 Feb;133(2):285-92. doi: 10.1016/j.thromres.2013.11.010. Epub 2013 Nov 25.
INTRODUCTION: Recently extracellular vesicles (exosomes, microparticles also referred to as microvesicles and apoptotic bodies) have attracted substantial interest as potential biomarkers and therapeutic vehicles. However, analysis of microparticles in biological fluids is confounded by many factors
PMID 24360116

Aberrant phenotype in Iranian patients with acute myeloid leukemia.

Jahedi M1, Shamsasenjan K2, Sanaat Z1, Aliparasti M3, Almasi S3, Mohamadian M1, Nejati B1, Kamalifar A4, Movassaghpour AA1.Author information 1Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.2Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.3Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. ; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. ; Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.4Students Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.AbstractPurpose: The aim of this study was to evaluate the incidence of aberrant phenotypes and possible prognostic value in peripheral and bone marrow blood mononuclear cells of Iranian patients with AML. Methods: 56 cases of de novo AML (2010-2012) diagnosed by using an acute panel of monoclonal antibodies by multiparametric flowcytometry. Immunophenotyping was done on fresh bone marrow aspirate and/or peripheral blood samples using the acute panel of MoAbs is stained with Phycoerythrin (PE) /fluorescein isothiocyanate (FITC), Allophycocyanin (APC) and Peridinin-chlorophyll protein complex (perCP). We investigated Co-expression of lymphoid-associated markers CD2, CD3, CD7, CD 10, CD19, CD20 and CD22 in myeloblasts. Results: Out of the 56 cases, 32 (57.1%) showed AP. CD7 was positive in 72.7% of cases in M1 and 28.5% in M2 but M3 and M4 cases lacked this marker. We detected CD2 in 58.35 of M1cases, 21.40% of M2 cases, 33.3 of M3 and 20% of M5; but M4 patients lacked this marker. The CBC analysis demonstrated a wide range of haemoglobin concentration, Platelet and WBC count which varied from normal to anaemia, thrombocytopenia to thrombocytosis and leukopenia to hyper leukocytosis. Conclusions: Our findings showed that CD7 and CD2 were the most common aberrant marker in Iranian patients with AML. However, we are not find any significant correlation between aberrant phenotype changing and MRD in our population. Taken together, this findings help to provide new insights in to the investigation of other aberrant phenotypes that may play roles in diagnosis and therapeutic of AML.
Advanced pharmaceutical bulletin.Adv Pharm Bull.2014;4(1):43-7. doi: 10.5681/apb.2014.007. Epub 2013 Dec 23.
Purpose: The aim of this study was to evaluate the incidence of aberrant phenotypes and possible prognostic value in peripheral and bone marrow blood mononuclear cells of Iranian patients with AML. Methods: 56 cases of de novo AML (2010-2012) diagnosed by using an acute panel of monoclonal antibodie
PMID 24409408