OBJECTIVE: To evaluate the effect of varicocele on oxidative stress, sperm mitochondrial respiratory efficiency, sperm morphology, and semen parameters.METHODS: A total of 20 patients with varicocele and 20 normozoospermic subjects without varicocele (control group) were recruited from a medical cen
In boar sperm capacitation L-lactate and succinate, but not pyruvate and citrate, contribute to the mitochondrial membrane potential increase as monitored via safranine O fluorescence.
Biochemical and biophysical research communications.Biochem Biophys Res Commun.2015 Jul 3;462(3):257-62. doi: 10.1016/j.bbrc.2015.04.128. Epub 2015 May 5.
Having ascertained using JC-1 as a probe that, in distinction with the controls, during capacitation boar sperm maintains high mitochondrial membrane potential (ΔΨ), to gain some insight into the role of mitochondria in capacitation, we monitored ΔΨ generation due to externally added metabolites
Ca(2+) is a Regulator of the WNK/OSR1/NKCC Pathway in a Human Salivary Gland Cell Line.
Park S1, Ku SK2, Ji HW1, Choi JH2, Shin DM1.
The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology.Korean J Physiol Pharmacol.2015 May;19(3):249-55. doi: 10.4196/kjpp.2015.19.3.249. Epub 2015 Apr 30.
Wnk kinase maintains cell volume, regulating various transporters such as sodium-chloride cotransporter, potassium-chloride cotransporter, and sodium-potassium-chloride cotransporter 1 (NKCC1) through the phosphorylation of oxidative stress responsive kinase 1 (OSR1) and STE20/SPS1-related proline/a
Previous work has shown increased uptake of hypotonically loaded rat RBCs by the spleen and liver "in vivo, " suggesting that the cells of MPS are involved in their elimination from the circulation. In order to elucidate the mechanism ...
Additionally, the total number of Cl-ions effluxed through the hypotonically activated channels was markedly similar to the total solute efflux during volume regulation. We therefore propose the hypotonically activated chloride ...