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1. 男性不妊症の評価 evaluation of male infertility

English Journal

Meta-analysis of the association of oestrogen receptor-beta gene RsaI (G/A) and AluI (A/G) polymorphisms with male infertility.

Cai Y1, Liu T, Li H, Xiong C.Author information 1Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.AbstractA more precise assessment of association of oestrogen receptor-beta genes RsaI(G/A) and AluI(A/G) polymorphisms with male infertility from current contradictory results is the aim of this meta-analysis including five RsaI and six AluI studies respectively. No association was observed between infertility and RsaI or AluI. In the stratified analysis by ethnicity, increased risk was found among Caucasians with GA versus GG (OR = 2.263, 95% CI = 1.073-4.776, I2 = 57.1%) and dominant model (OR = 2.117, 95% CI = 1.018-4.403, I2 = 49.0%) of RsaI. It was not observed for AluI. In the stratified analysis by infertility subtypes, a reduced risk in GA of AluI was observed among azoospermia or severe oligospermia (GA versus AA: OR = 0.686, 95% CI = 0.498-0.945, I2 = 21.2%; recessive model: OR = 1.403, 95% CI = 1.056-1.864, I2 = 31.7%), and reduced risk was in recessive model (OR = 0.650, 95% CI = 0.446-0.948, I2 = 0.0%) of subtypes, except for azoospermia or severe oligospermia. However, this finding was not observed in RsaI. The meta-analysis showed GA and GG of AluI are possibly resistant factors for spermatogenesis dysfunction and deteriorated sperm quality.
Andrologia.Andrologia.2014 Mar 23. doi: 10.1111/and.12254. [Epub ahead of print]
A more precise assessment of association of oestrogen receptor-beta genes RsaI(G/A) and AluI(A/G) polymorphisms with male infertility from current contradictory results is the aim of this meta-analysis including five RsaI and six AluI studies respectively. No association was observed between inferti
PMID 24655068

Melatonin in testes of infertile men: evidence for anti-proliferative and anti-oxidant effects on local macrophage and mast cell populations.

Rossi SP1, Windschuettl S, Matzkin ME, Terradas C, Ponzio R, Puigdomenech E, Levalle O, Calandra RS, Mayerhofer A, Frungieri MB.Author information 1Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina; Departamento de Bioquímica Humana, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.AbstractMelatonin acting through the hypothalamus and pituitary regulates testicular function. In addition, direct actions of melatonin at the testicular level have been recently suggested. We have described that melatonin inhibits androgen production in hamster Leydig cells via melatonin subtype 1a (mel1a) receptors and the local corticotrophin-releasing hormone (CRH) system. The initial events of the melatonin/CRH signalling pathway have also been established. Melatonin and all components of the melatonergic/CRH system were also detected in Leydig cells of infertile men. This study attempted to search for additional targets of melatonin in the human testis, and to investigate the effects of melatonin on proliferation and the oxidative state in these novel target cells. To this aim, evaluation of human testicular biopsies of patients suffering from hypospermatogenesis or Sertoli cell only syndrome and cell culture studies were performed. Melatonergic receptors were found in macrophages (MACs) and mast cells (MCs) of the human testis. In biopsies of patients suffering idiopathic infertility, melatonin testicular concentrations were negatively correlated with MAC number per mm2 and TNFα, IL1β and COX2 expression, but positively correlated with the expression of the anti-oxidant enzymes SOD1, peroxiredoxin 1 and catalase. Melatonin inhibited proliferation and the expression of pro-inflammatory cytokines and cyclooxygenase 2 (COX2) in both the human non-testicular THP-1 MAC cell line and primary cell cultures of hamster testicular MACs. In the human HMC-1 MC line, melatonin increased the expression of anti-oxidant enzymes and decreased reactive oxygen species (ROS) generation. The results reveal new testicular targets of melatonin and describe anti-proliferative and anti-inflammatory effects of this hormone on testicular MACs. Furthermore, melatonin might provide protective effects against oxidative stress in testicular MCs.
Andrology.Andrology.2014 Mar 21. doi: 10.1111/j.2047-2927.2014.00207.x. [Epub ahead of print]
Melatonin acting through the hypothalamus and pituitary regulates testicular function. In addition, direct actions of melatonin at the testicular level have been recently suggested. We have described that melatonin inhibits androgen production in hamster Leydig cells via melatonin subtype 1a (mel1a)
PMID 24659586

Pathological changes of testicular tissue in normal adult mice: A retrospective analysis.

Xie BG1, Li J2, Zhu WJ1.Author information 1Department of Developmental and Regenerative Biology, College of Life Science and Technology, Jinan University, Guangzhou, Guangdong 510632, P.R. China.2Department of Pathophysiology, Medical College, Jinan University, Guangzhou, Guangdong 510632, P.R. China.AbstractMouse testicular experimental models are widely used in the study of andrology, reproductive toxicology and pharmacology. Under physiological conditions, a normal adult mouse is usually considered to have normal testes. However, whether normal adult mouse testes exhibit pathological changes has not been evaluated. The objective of this study was to investigate the pathological changes of testicular tissues in normal adult mice. A retrospective analysis of 720 adult male Kunming mice, used in previous studies as controls, was performed. Bilateral testicular tissues were stained with hematoxylin and eosin for pathological examinations. Among the 720 mice, nine had abnormal testes, an incidence of 1.3%. The nine mice with abnormal testes included two with microrchidia (22.2%) while the others had a normal testicular size. The observed pathological changes associated with microrchidia were seminiferous epithelial vacuolation, spermatogenesis arrest at the spermatocyte stage and the absence of sperm in all tubules. In other abnormal testes, pathological alterations included seminiferous epithelial vacuolation, severe hypospermatogenesis and symplasts composed of collapsed spermatids in tubules. The results demonstrate that normal adult male mice exhibit testicular pathological changes. Therefore, the possibility of abnormal testes in normal adult mice must be considered when using mice to establish a testicular experimental model.
Experimental and therapeutic medicine.Exp Ther Med.2014 Mar;7(3):654-656. Epub 2014 Jan 9.
Mouse testicular experimental models are widely used in the study of andrology, reproductive toxicology and pharmacology. Under physiological conditions, a normal adult mouse is usually considered to have normal testes. However, whether normal adult mouse testes exhibit pathological changes has not
PMID 24520262

Japanese Journal

Aberrant methylation of the TDMR of the GTF2A1L promoter does not affect fertilisation rates via TESE in patients with hypospermatogenesis

Sugimoto Kazuhiro,Koh Eitetsu,Iijima Masashi,Taya Masaki,Maeda Yuji,Namiki Mikio
Asian Journal of Andrology 15(5), 634-639, 2013-09
… Based on histological criteria, 26 of the 86 patients had normal spermatogenesis (controls), 17 had hypospermatogenesis and 26 had a Sertoli cell-only phenotype or tubular sclerosis. … Patients with hypospermatogenesis were divided into two subgroups: high DNA methylation (HM, n=5) and low DNA methylation (LM, n=12). …
NAID 120005334019

Histopathology of Incidental Findings in Beagles Used in Toxicity Studies

Sato Junko,Doi Takuya,Wako Yumi [他],Hamamura Masao,Kanno Takeshi,Tsuchitani Minoru,Narama Isao
Journal of Toxicologic Pathology 25(1), 103-134, 2012
… Spontaneous focal hypospermatogenesis and lobular Sertoli-cell-only seminiferous tubules occurring frequently in beagles must be distinguished from drug-induced damage of the seminiferous tubules in toxicity studies. …
NAID 130001884591

Toxic responses to deltamethrin (DM) low doses on gonads, sex hormones and lipoperoxidation in male rats following subcutaneous treatments

Issam Chargui,Samir Haouem,Zohra Haouas [他],Monia Zaouali,Hassen Ben Cheikh
Journal of toxicological sciences 34(6), 663-670, 2009-12-01
… Our study mentioned an hypospermatogenesis within the testes accompanied by some apoptotic figures in particular cell fragments into the seminiferous tubules (ST)' lumen. …
NAID 110007485676

Related Pictures

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