English Journal

Self-Assembling Nano-Architectures Created from a Protein Nano-Building Block Using an Intermolecularly Folded Dimeric de Novo Protein.

Kobayashi N1, Yasase K, Sato T, Unzai S2, Hecht MH3, Arai R4.
Journal of the American Chemical Society.J Am Chem Soc.2015 Jul 14. [Epub ahead of print]
The design of novel proteins that self-assemble into supramolecular complexes is an important step in the development of synthetic biology and nanotechnology. Recently, we described the three-dimensional structure of WA20, a de novo protein that forms an intermolecularly folded dimeric 4-helix bundl
PMID 26120734

Endocytosis of GABAC receptors depends on subunit composition and is regulated by protein kinase C-ζ and protein phosphatase 1.

Linck L1, Binder J1, Haynl C1, Enz R1.
Journal of neurochemistry.J Neurochem.2015 Jul;134(2):233-46. doi: 10.1111/jnc.13126. Epub 2015 Apr 27.
Neuronal excitability depends on the surface concentration of neurotransmitter receptors. Type C gamma-aminobutyric acid receptors (GABAC R) are composed of ρ subunits that are highly expressed in the retina. Molecular mechanisms that guide the surface concentration of this receptor type are largel
PMID 25868914

Supramolecular chemical shift reagents inducing conformational transitions: NMR analysis of carbohydrate homooligomer mixtures.

Beeren SR1, Meier S.
Chemical communications (Cambridge, England).Chem Commun (Camb).2015 Feb 21;51(15):3073-6. doi: 10.1039/c4cc09710f.
We introduce the concept of supramolecular chemical shift reagents as a tool to improve signal resolution for the NMR analysis of homooligomers. Non-covalent interactions with the shift reagent can constrain otherwise flexible analytes inducing a conformational transition that results in signal sepa
PMID 25604578