発現クローニング、発現クローニング法
WordNet
- (genetics) the process of expressing a gene
- the feelings expressed on a persons face; "a sad expression"; "a look of triumph"; "an angry face" (同)look, aspect, facial_expression, face
- the act of forcing something out by squeezing or pressing; "the expression of milk from her breast"
- expression without words; "tears are an expression of grief"; "the pulse is a reflection of the hearts condition" (同)manifestation, reflection, reflexion
- the communication (in speech or writing) of your beliefs or opinions; "expressions of good will"; "he helped me find verbal expression for my ideas"; "the idea was immediate but the verbalism took hours" (同)verbal expression, verbalism
- a general term for the research activity that creates a copy of some biological entity (a gene or organism or cell)
PrepTutorEJDIC
- 〈U〉〈C〉(思想・意見・考えなどを)『言葉で表すこと』,(…の)『表現』《+『of』+『名』》 / 〈C〉(考え・気持ちなどの)『現れ』,印《+『of』+『名』》 / 〈C〉(考え・気持ちなどを表す)『顔つき』,表情《+『of』+『名』》 / 〈U〉(考え・気持ちなどを表す)声の調子 / 〈C〉語句,言い回し,表現法 / 〈C〉(数量・運算などを示す)式
Wikipedia preview
出典(authority):フリー百科事典『ウィキペディア(Wikipedia)』「2014/04/18 09:17:06」(JST)
[Wiki en表示]
|
This article does not cite any references or sources. Please help improve this article by adding citations to reliable sources. Unsourced material may be challenged and removed. (October 2007) |
Expression cloning is a technique in DNA cloning that uses expression vectors to generate a library of clones, with each clone expressing one protein. This expression library is then screened for the property of interest and clones of interest recovered for further analysis. An example would be using an expression library to isolate genes that could confer antibiotic resistance.
Expression vectors
Expression vectors are a specialized type of cloning vector in which the transcriptional and translational signals needed for the regulation of the gene of interest are included in the cloning vector. The transcriptional and translational signals may be synthetically created to make the expression of the gene of interest easier to regulate.
Purpose
Usually the ultimate aim of expression cloning is to produce large quantities of specific proteins. To this end, a bacterial expression clone may include a ribosome binding site (Shine-Dalgarno sequence) to enhance translation of the gene of interest's mRNA, a transcription termination sequence, or, in eukaryotes, specific sequences to promote the post-translational modification of the protein product.
See also
- Molecular cell biology
- genetics
- gene expression
- Transcription (genetics)
- translation
- λ phage
- pBR322
UpToDate Contents
全文を閲覧するには購読必要です。 To read the full text you will need to subscribe.
English Journal
- Fine Mapping Identifies a New QTL for Brown Rice Rate in Rice (Oryza Sativa L.).
- Ren D1, Rao Y1,2, Huang L1, Leng Y1, Hu J1, Lu M2, Zhang G1, Zhu L1, Gao Z1, Dong G1, Guo L1, Qian Q3, Zeng D4.
- Rice (New York, N.Y.).Rice (N Y).2016 Dec;9(1):4. doi: 10.1186/s12284-016-0076-7. Epub 2016 Feb 4.
- BACKGROUND: High yield and quality determine the commercial potential of rice variety. Brown rice rate (BRR) is a key factor ensuring grain yield and quality in rice. So far, there were few reports about the genes that directly controlled the BRR in rice. Therefore, dissecting the genetic mechanism
- PMID 26847792
- Identification of novel isoforms of dairy goat EEF1D and their mRNA expression characterization.
- Zhang S1, Wu X2, Pan C3, Lei C3, Dang R3, Chen H3, Lan X4.
- Gene.Gene.2016 Apr 25;581(1):14-20. doi: 10.1016/j.gene.2016.01.013. Epub 2016 Jan 12.
- Eukaryotic translation elongation factor 1 delta (EEF1D) gene encodes guanine nucleotide exchange protein eEF1Bδ, which participates in the eukaryotic protein synthesis, and plays important roles in regulating cell cycling and milk production. This study firstly focused on detecting the isoforms of
- PMID 26794801
- Construction of Mycobacterium tuberculosis ESAT-6 fused to human Fcγ of IgG1: To target FcγR as a delivery system for enhancement of immunogenicity.
- Soleimanpour S1, Mohammadi MA1, Ghazvini K1, Jamehdar SA1, Sadeghian H1, Taghiabadi M1, Rezaee SA2.
- Gene.Gene.2016 Apr 15;580(2):111-7. doi: 10.1016/j.gene.2016.01.009. Epub 2016 Jan 8.
- In order to prevent spreading of Mycobacterium tuberculosis (Mtb), it is necessary to discover effective vaccines, fast and reliable diagnosis, and appropriate treatment schemes. In the present study, an Fc-tagged recombinant Mtb-ESAT-6 was produced to make a selective delivery system for promoting
- PMID 26778208
Japanese Journal
- cDNA cloning of an alginate lyase from a marine gastropod Aplysia kurodai and assessment of catalytically important residues of this enzyme.
- Rahman Mohammad Matiur,Inoue Akira,Tanaka Hiroyuki,Ojima Takao
- Biochimie 93(10), 1720-1730, 2011-10
- … An Escherichia coli BL21(DE3)-pCold I expression system for recombinant AkAly30 (recAkAly30) was constructed and site-directed mutagenesis was performed to assess catalytically important amino-acid residues which had been suggested in abalone and Chlorella virus PL-14 enzymes. …
- NAID 120003295083
- Cloning, expression and purification of the anion exchanger 1 homologue from the basidiomycete Phanerochaete chrysosporium.
- Tokuda Natsuko,Igarashi Kiyohiko,Shimamura Tatsuro,Yurugi-Kobayashi Takami,Shiroishi Mitsunori,Ito Keisuke,Sugawara Taishi,Asada Hidetsugu,Murata Takeshi,Nomura Norimichi,Iwata So,Kobayashi Takuya
- Protein expression
- Anion exchangers are membrane proteins that have been identified in a wide variety of species, where they transport Cl(-) and HCO3(-) across the cell membrane. In this study, we cloned an anion-exchan …
- NAID 120003255719
Related Links
- Genlantis offers a wide selection of cloning and expression tools that assist in expressing proteins to study genes. Purchase your cloning supplies today. ... Cloning and Expression The general aim of cloning is to express specific ...
- Expression Cloning Jun-yong Noh Ulrich Neumann noh@usc.edu uneumann@usc.edu Computer Graphics and Immesive Technologies Laboratory Computer Science Department Integrated Media Systems Center University of ...
Related Pictures
★リンクテーブル★
[★]
- 英
- expression cloning
- 関
- 発現クローニング法
[★]
- 英
- expression cloning
- 関
- 発現クローニング
[★]
- 関
- Eq.、equation、exert、express、facial expression、formula、formulae、level of expression、manifestation、represent、representation、representational