English Journal

Demasking of epithelial cell adhesion molecule (EpCAM) on circulating epithelial tumor cells by Tween®20 treatment in breast cancer patients.

Hekimian K, Stein EL, Pachmann U, Pachmann K.AbstractAbstract Background: The epithelial cell adhesion molecule (EpCAM) embedded in the plasma membrane of circulating epithelial tumor cells (CETC) is used for detection and enrichment of circulating tumor cells in peripheral blood and as a target for anti-epithelial antibodies elicited during immune response in anti-tumor immunization. Although an efficient immune response against EpCAM can be generated, the clinical application of such approaches has not been successful so far and the detection of circulating epithelial cells is highly variable. One reason for these discrepancies may be that not all circulating tumor cells are equally accessible for the specific antibody. A possible reason might be masking of EpCAM by glycoproteins or membrane lipoproteins preventing antibody binding. Methods: We have tested the application of detergents as demasking agents known to be successful in demasking red blood cell epitopes and determined how and in which way they affect integral membrane proteins and membrane lipids. Results: The results showed that the polysorbate Tween®20, a non-ionic detergent like organic solvent is able to demask EpCAM on CETC and makes it better accessible to its specific antibody retaining at the same time full cell viability. Conclusions: The data presented in this study suggest that EpCAM is present on part of circulating tumor cells in a masked form and that it is possible to demask EpCAM on CETC of breast cancer patients using Tween®20 treatment. But further studies are needed to elucidate the mechanism of demasking.
Clinical chemistry and laboratory medicine : CCLM / FESCC.Clin Chem Lab Med.2012 Dec 7;50(4):701-8. doi: 10.1515/cclm.2011.812.
Abstract Background: The epithelial cell adhesion molecule (EpCAM) embedded in the plasma membrane of circulating epithelial tumor cells (CETC) is used for detection and enrichment of circulating tumor cells in peripheral blood and as a target for anti-epithelial antibodies elicited during immune re
PMID 22505561

Cell biological characterization of a multidomain adaptor protein, ArgBP2, in epithelial NMuMG cells, and identification of a novel short isoform.

Murase K, Ito H, Kanoh H, Sudo K, Iwamoto I, Morishita R, Soubeyran P, Seishima M, Nagata K.SourceDepartment of Dermatology, Gifu University Graduate School of Medicine, Gifu, Japan.
Medical molecular morphology.Med Mol Morphol.2012 Dec;45(1):22-8. Epub 2012 Mar 20.
ArgBP2 is a member of the SoHo (sorbin-homology) family of adaptor proteins believed to play roles in cell adhesion, cytoskeletal organization, and signaling. We show here a novel splicing isoform of ArgBP2, i.e., ArgBP2™, composed of only three SH3 (src-homology 3) domains and structurally simila
PMID 22431180

Japanese Journal

P-12. An established murine intestinal epitheliocyte (MIE) cell line(Abstracts of the International Symposium on Recent Advances in Animal Science(IS-RAAS),Joint meeting of 2^<nd> IS-AS and 3^<rd> IS-IFS)

Kanaya T.,Aso H.,Miyazawa K.,Minashima T.,Kido T.,Watanabe K.,Ohwada S.,Yamaguchi T.
Tohoku journal of agricultural research 56(1/2), 41, 2005-11-25
NAID 110004811288