English Journal

Yoshida T, Mitsukura K, Mizutani T, Nakashima R, Shimizu Y, Kawabata H, Nagasawa T.SourceDepartment of Biomolecular Science, Gifu University, Yanagido 1-1, Gifu, 501-1193, Japan, toyosida@gifu-u.ac.jp.
Biotechnology letters.Biotechnol Lett.2013 Jan 4. [Epub ahead of print]
The nitrilase gene of Rhodococcus rhodochrous J1 was expressed in Escherichia coli using the expression vector, pKK223-3. The recombinant E. coli JM109 cells hydrolyzed enantioselectively 2-methyl-2-propylmalononitrile to form (S)-2-cyano-2-methylpentanoic acid (CMPA) with 96 % e.e. Under optimized
PMID 23288294

Asymmetric Total Syntheses of (-)-Penicipyrone and (-)-Tenuipyrone via Biomimetic Cascade Intermolecular Michael Addition/Cycloketalization.

Song L, Yao H, Zhu L, Tong R.SourceDepartment of Chemistry, The Hong Kong University of Science and Technology , Clear Water Bay, Kowloon, Hong Kong, China.
Organic letters.Org Lett.2013 Jan 4;15(1):6-9. doi: 10.1021/ol303071t. Epub 2012 Dec 18.
The first total syntheses of (-)-penicipyrone and (-)-tenuipyrone were accomplished enantioselectively in 12 steps with an 11% yield and 6 steps with a 28% yield from the known 4-((tert-butyldimethylsilyl)oxy)-cyclopent-2-enone, respectively, by developing a biomimetic bimolecular cascade cyclizatio
PMID 23249160