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Selenocystamine improves protein accumulation in chloroplasts of eukaryotic green algae.

Ferreira-Camargo LS1, Tran M, Beld J, Burkart MD, Mayfield SP.
AMB Express.AMB Express.2015 Dec;5(1):126. doi: 10.1186/s13568-015-0126-3. Epub 2015 Jul 3.
Eukaryotic green algae have become an increasingly popular platform for recombinant proteins production. In particular, Chlamydomonas reinhardtii, has garnered increased attention for having the necessary biochemical machinery to produce vaccines, human antibodies and next generation cancer targetin
PMID 26137911

Molecular Characterization of Endoplasmic Reticulum Oxidoreductin 1 from Bombyx mori.

Seo M1, Ryou HJ2, Yun EY3, Goo TW4.
International journal of molecular sciences.Int J Mol Sci.2015 Nov 5;16(11):26520-26529.
We isolated a complementary DNA (cDNA) clone encoding endoplasmic reticulum oxidoreductin 1 (bERO1, a specific oxidant of protein disulfide isomerase (PDI)) from Bombyx mori. This protein has a putative open reading frame (ORF) of 489 amino acids and a predicted size of 57.4 kDa. Although bERO1 prot
PMID 26556347

Zhou J, Wu Y, Wang L, Rauova L, Hayes VM, Poncz M, Essex DW.
The Journal of clinical investigation.J Clin Invest.2015 Nov 3;2015. pii: 80319. doi: 10.1172/JCI80319.
Protein disulfide isomerase (PDI) has two distinct CGHC redox-active sites; however, the contribution of these sites during different physiologic reactions, including thrombosis, is unknown. Here, we evaluated the role of PDI and redox-active sites of PDI in thrombosis by generating mice with blood
PMID 26529254

Efficient folding/assembly in Chinese hamster ovary cells is critical for high quality (low aggregate content) of secreted trastuzumab as well as for high production : Stepwise multivariate regression analyses

Efficient folding/assembly in Chinese hamster ovary cells is critical for high quality (low aggregate content) of secreted trastuzumab as well as for high production : Stepwise multivariate regression analyses(CELL AND TISSUE ENGINEERING)

Ishii Yoichi,Murakami Junko,Sasaki Kazue,Tsukahara Masayoshi,Wakamatsu Kaori
Journal of bioscience and bioengineering 118(2), 223-230, 2014-08
… The aggregate content was found to positively associate with protein disulfide isomerase mRNA level and negatively with light chain secreted into culture media, specific production rate, intracellular light chain content, and specific growth rate. …
NAID 110009840789

Role of disulfide bond isomerase DsbC, calcium ions, and hemin in cell-free protein synthesis of active manganese peroxidase isolated from Phanerochaete chrysosporium