cytochrome P450 enzymes

出典: meddic

CYPs

UpToDate Contents

全文を閲覧するには購読必要です。 To read the full text you will need to subscribe.

英文文献

  • 1,3-Dichloro-2-propanol inhibits progesterone production through the expression of steroidogenic enzymes and cAMP concentration in Leydig cells.
  • Sun J1, Bai S2, Bai W3, Zou F2, Zhang L2, Li G2, Hu Y2, Li M2, Yan R2, Su Z4, Huang Y2.Author information 1Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China; Department of Food Science and Engineering, Department of Developmental and Regenerative Biology, Biopharmaceutical R&D Center, Jinan University, Guangzhou 510632, China.2Department of Food Science and Engineering, Department of Developmental and Regenerative Biology, Biopharmaceutical R&D Center, Jinan University, Guangzhou 510632, China.3Department of Food Science and Engineering, Department of Developmental and Regenerative Biology, Biopharmaceutical R&D Center, Jinan University, Guangzhou 510632, China. Electronic address: baiweibin@163.com.4Department of Food Science and Engineering, Department of Developmental and Regenerative Biology, Biopharmaceutical R&D Center, Jinan University, Guangzhou 510632, China. Electronic address: su_zhijian@126.com.Abstract1,3-Dichloro-2-propanol (1,3-DCP) is a well-known food processing contaminant that has been shown to impede male reproductive function. However, its mechanism of action remains elusive. In this study, the effects of 1,3-DCP on progesterone production were investigated using the R2C Leydig cell model. 1,3-DCP significantly reduced cell viability from 7.48% to 97.4% at doses comprised between 0.5 and 6mM. Single cell gel/comet assays and atomic force microscopy assays showed that 1,3-DCP induced early phase cell apoptosis. In addition, 1,3-DCP significantly reduced progesterone production detected by radioimmunoassay (RIA). The results from quantitative polymerase chain reaction and western blotting demonstrated that the mRNA expression levels of steroidogenic acute regulatory protein (StAR), cytochrome P450 side-chain cleavage enzyme and 3β-hydroxysteroid dehydrogenase were significantly down-regulated in R2C cells. Particularly, the change rhythm of Star expression was highly consistent with progesterone production. Furthermore, the cyclic adenosine monophosphate (cAMP) and the mitochondrial membrane potential mediated by ROS, which are involved in regulating progesterone synthesis were also decreased in response to the 1,3-DCP treatment. Overall, the data presented here suggested that 1,3-DCP interferes with the male steroidogenic capacity mainly by down-regulating the level of cAMP and the key enzymes involved in the androgen synthesis pathway.
  • Food chemistry.Food Chem.2014 Jul 1;154:330-6. doi: 10.1016/j.foodchem.2014.01.017. Epub 2014 Jan 14.
  • 1,3-Dichloro-2-propanol (1,3-DCP) is a well-known food processing contaminant that has been shown to impede male reproductive function. However, its mechanism of action remains elusive. In this study, the effects of 1,3-DCP on progesterone production were investigated using the R2C Leydig cell model
  • PMID 24518350
  • The Influence of the CYP3A4*22 Polymorphism on Serum Concentration of Quetiapine in Psychiatric Patients.
  • van der Weide K1, van der Weide J.Author information 1From the *St Jansdal Hospital, Department of Clinical Chemistry, Harderwijk; and †Psychiatric Hospital GGz Centraal, location Veldwijk, Ermelo, the Netherlands.AbstractBACKGROUND: Besides dietary, hormonal, or pathological factors, mutations in cytochrome P450 enzymes are thought to be responsible for the interindividual differences in serum concentrations of cytochrome P450 (CYP450)-dependent drugs. Cytochrome P450 3A4 (CYP3A4) is involved in the metabolism of greater than 50% of the prescribed drugs. Recently, a new single-nucleotide polymorphism (SNP) was found (CYP3A4*22), which results in a decreased enzyme activity, in contrast to the other known SNPs in CYP3A4. We investigated to which degree the CYP3A4*22 SNP affects serum concentrations of patients receiving quetiapine, a drug exclusively metabolized by CYP3A4.
  • Journal of clinical psychopharmacology.J Clin Psychopharmacol.2014 Apr;34(2):256-60. doi: 10.1097/JCP.0000000000000070.
  • BACKGROUND: Besides dietary, hormonal, or pathological factors, mutations in cytochrome P450 enzymes are thought to be responsible for the interindividual differences in serum concentrations of cytochrome P450 (CYP450)-dependent drugs. Cytochrome P450 3A4 (CYP3A4) is involved in the metabolism of gr
  • PMID 24525658
  • Inhibition of human cytochrome P450 enzymes by hops (Humulus lupulus) and hop prenylphenols.
  • Yuan Y1, Qiu X1, Nikolić D1, Chen SN1, Huang K1, Li G1, Pauli GF1, van Breemen RB2.Author information 1UIC/NIH Center for Botanical Dietary Supplements Research, Department of Medicinal Chemistry and Pharmacognosy, University of Illinois College of Pharmacy, Chicago, IL 60612-72312, United States.2UIC/NIH Center for Botanical Dietary Supplements Research, Department of Medicinal Chemistry and Pharmacognosy, University of Illinois College of Pharmacy, Chicago, IL 60612-72312, United States. Electronic address: breemen@uic.edu.AbstractAs hops (Humulus lupulus L.) are used in the brewing of beer and by menopausal women as estrogenic dietary supplements, the potential for hop extracts and hop constituents to cause drug-botanical interactions by inhibiting human cytochrome P450 enzymes was investigated. Inhibition of major human cytochrome P450 enzymes by a standardized hop extract and isolated hop prenylated phenols was evaluated using a fast and efficient assay based on ultrahigh pressure liquid chromatography-tandem mass spectrometry. The hop extract at 5μg/mL inhibited CYP2C8 (93%), CYP2C9 (88%), CYP2C19 (70%), and CYP1A2 (27%) with IC50 values of 0.8, 0.9, 3.3, and 9.4μg/mL, respectively, but time-dependent inactivation was observed only for CYP1A2. Isoxanthohumol from hops was the most potent inhibitor of CYP2C8 with an IC50 of 0.2μM, whereas 8-prenylnaringenin was the most potent inhibitor of CYP1A2, CYP2C9 and CYP2C19 with IC50 values of 1.1μM, 1.1μM and 0.4μM, respectively. Extracts of hops contain prenylated compounds such as the flavanones isoxanthohumol and 8-prenylnaringenin and the chalcone xanthohumol that can inhibit CYP450s, especially the CYP2C family, which may affect the efficacy and safety of some CYP2C substrate drugs when co-administered.
  • European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences.Eur J Pharm Sci.2014 Mar 12;53:55-61. doi: 10.1016/j.ejps.2013.12.003. Epub 2013 Dec 14.
  • As hops (Humulus lupulus L.) are used in the brewing of beer and by menopausal women as estrogenic dietary supplements, the potential for hop extracts and hop constituents to cause drug-botanical interactions by inhibiting human cytochrome P450 enzymes was investigated. Inhibition of major human cyt
  • PMID 24342125

和文文献

  • A new in vivo analysis model to detect sexually dimorphic rat liver cytochrome P450 gene expression dependent on growth hormone secretory patterns
  • Identification of candidate target Cyp genes for microRNAs whose expression is altered by PCN and TCPOBOP, representative ligands of PXR and CAR
  • Correlation between Activity and Molecular Structure around the Active Center of Cytochrome P450cam Conjugates

関連リンク

The cytochrome P450 superfamily (officially abbreviated as CYP) is a large and diverse group of enzymes that catalyze .... Cytochrome P450 enzymes are present in most tissues of the body, and play important roles in hormone synthesis and ...
It is know that the gene for cytochrome P-450 has existed for more then 3.5 billion years. This indicates drug metabolism by the P-450 system is a new and secondary role for these enzyme systems. The primary role for the P450 system seems ...

関連画像

Cytochrome p-450 enzyme system; Cytochrome Normally, cytochromes P450 areThe role of cytochrome P450 enzymes in The role of CYP3A4 and p-glycoprotein in Cytochrome p45018.2 Cytochrome P450 enzymes


★リンクテーブル★
関連記事enzyme」「cytochrome

enzyme」

  [★] 酵素

WordNet   license wordnet

「any of several complex proteins that are produced by cells and act as catalysts in specific biochemical reactions」

PrepTutorEJDIC   license prepejdic

「酵素」

cytochrome」

  [★] シトクロム

WordNet   license wordnet

「(biochemistry) a class of hemoprotein whose principal biological function is electron transfer (especially in cellular respiration)」




★コメント★

[メモ入力エリア]
※コメント5000文字まで
ニックネーム:
コメント:




表示
個人用ツール


  meddic.jp

リンク
連絡