WordNet
- grow in a special preparation; "the biologist grows microorganisms"
- (biology) the growing of microorganisms in a nutrient medium (such as gelatin or agar); "the culture of cells in a Petri dish"
- the raising of plants or animals; "the culture of oysters"
- a particular society at a particular time and place; "early Mayan civilization" (同)civilization, civilisation
- the attitudes and behavior that are characteristic of a particular social group or organization; "the developing drug culture"; "the reason that the agency is doomed to inaction has something to do with the FBI culture"
- the tastes in art and manners that are favored by a social group
PrepTutorEJDIC
- 〈U〉『教養』;修養;教化 / 〈C〉〈U〉『文化』(主として精神面をさし,生活・習慣・物の考え方などを含む)Greek cultureギリシア文化 / 〈U〉(…の)『耕作』;『栽培』,養殖,培養《+『of』+『名』》
- 神経単位,神経細胞,ニューロン,ノイロン
- 教養のある,洗練された,上品な / 栽培(養殖)された
UpToDate Contents
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- 1. 筋萎縮性側索硬化症およびその他の運動ニューロン疾患の臨床的特徴 clinical features of amyotrophic lateral sclerosis and other forms of motor neuron disease
- 2. 筋萎縮性側索硬化症およびその他の運動ニューロン疾患の診断 diagnosis of amyotrophic lateral sclerosis and other forms of motor neuron disease
- 3. 重症筋無力症の鑑別診断 differential diagnosis of myasthenia gravis
- 4. 成人における詳細な神経学的検査 the detailed neurologic examination in adults
- 5. 筋萎縮性側索硬化症の疫学および病因 epidemiology and pathogenesis of amyotrophic lateral sclerosis
English Journal
- Three-dimensional self-assembling peptide matrix enhances the formation of embryoid bodies and their neuronal differentiation.
- Li Q1, Chow KL, Chau Y.Author information 1Department of Chemical and Biomolecular Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, Republic of China.AbstractWe have tailored the properties of a self-assembling peptide (SAP) matrix to direct embryonic stem cells towards neuronal differentiation by adopting a three-dimensional (3D) culture, matching mechanical strength with that of neural tissue, and incorporating fixed laminin-derived pentapeptide signals (IKVAV). We report here that such a matrix alone can induce mouse embryonic stem (ES) cells to first develop into embryoid body (EB) and increase their propensity for subsequent neuronal differentiation. Embryoid bodies were observed by day 5 of culture in SAP matrix. βIII-tubulin as an early neuronal commitment marker was more prominently detected in cells cultured in the matrix containing IKVAV signals. Interestingly, ES-derived cells did not display distinct neuron morphology within the 3D culture; however, 55 ± 10% of those cells within IKVAV conjugated matrix and 38 ± 6% of those within base matrix displayed higher potential towards neuronal differentiation after 7 days. When retrieved and recultured on a tissue culture plate, they exhibited extended neurite outgrowths and networks in the absence of any additional neuronal differentiation growth factor. The up-regulated expression of neuronal development markers (MAP2 and MeCP2) and the down-regulation of glial marker (GFAP) support that further neuronal differentiation takes place upon reculture. The results showed that an artificial matrix composed of designer SAPs could prompt the formation of EB and provides the cues favoring neuronal differentiation of ES cells. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1991-2000, 2014.
- Journal of biomedical materials research. Part A.J Biomed Mater Res A.2014 Jun;102(6):1991-2000. doi: 10.1002/jbm.a.34876. Epub 2013 Jul 30.
- We have tailored the properties of a self-assembling peptide (SAP) matrix to direct embryonic stem cells towards neuronal differentiation by adopting a three-dimensional (3D) culture, matching mechanical strength with that of neural tissue, and incorporating fixed laminin-derived pentapeptide signal
- PMID 23894036
- M2 receptors exert analgesic action on DRG sensory neurons by negatively modulating VR1 activity.
- De Angelis F1, Marinelli S, Fioretti B, Catacuzzeno L, Franciolini F, Pavone F, Tata AM.Author information 1Department of Biology and Biotechnologies C. Darwin, "Sapienza" University of Rome, Rome, Italy.AbstractThe peripheral application of the M2 cholinergic agonist arecaidine on sensory nerve endings shows anti-nociceptive properties. In this work, we analyze in vitro, the mechanisms downstream M2 receptor activation causing the analgesic effects, and in vivo the effects produced by M2 agonist arecaidine administration on nociceptive responses in a murine model of nerve growth factor (NGF)-induced pain. Cultured DRG neurons treated with arecaidine showed a decreased level of VR1 and SP transcripts. Conversely, we found an increased expression of VR1 and SP transcripts in DRG from M2/M4(-/-) mice compared to WT and M1(-/-) mice, confirming the inhibitory effect in particular of M2 receptors on SP and VR1 expression. Patch-clamp experiments in the whole-cell configuration showed that arecaidine treatment caused a reduction of the fraction of capsaicin-responsive cells, without altering the mean capsaicin-activated current in responsive cells. We also demonstrated that arecaidine prevents PKCϵ translocation to the plasma membrane after inflammatory agent stimulation, mainly in medium-small sensory neurons. Finally, in mice, we have observed that intraperitoneal injection of arecaidine reduces VR1 expression blocking hyperalgesia and allodynia caused by NGF intraplantar administration. In conclusion, our data demonstrate that in vivo M2 receptor activation induces desensitization to mechanical and heat stimuli by a down-regulation of VR1 expression and by the inhibition of PKCϵ activity hindering its translocation to the plasma membrane, as suggested by in vitro experiments.
- Journal of cellular physiology.J Cell Physiol.2014 Jun;229(6):783-90. doi: 10.1002/jcp.24499.
- The peripheral application of the M2 cholinergic agonist arecaidine on sensory nerve endings shows anti-nociceptive properties. In this work, we analyze in vitro, the mechanisms downstream M2 receptor activation causing the analgesic effects, and in vivo the effects produced by M2 agonist arecaidine
- PMID 24166293
- Curcumol from Rhizoma Curcumae suppresses epileptic seizure by facilitation of GABA(A) receptors.
- Ding J1, Wang JJ2, Huang C2, Wang L2, Deng S3, Xu TL2, Ge WH4, Li WG5, Li F6.Author information 1Department of Chinese Materia Medica, College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China; Department of Developmental and Behavioral Pediatrics, Shanghai Institute of Pediatric Translational Medicine, Shanghai Children's Medical Center, Ministry of Education-Shanghai Key Laboratory of Children's Environmental Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200129, China; Departments of Anatomy and Embryology, Biochemistry and Molecular Cell Biology, Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Institute of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.2Departments of Anatomy and Embryology, Biochemistry and Molecular Cell Biology, Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Institute of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.3Department of Developmental and Behavioral Pediatrics, Shanghai Institute of Pediatric Translational Medicine, Shanghai Children's Medical Center, Ministry of Education-Shanghai Key Laboratory of Children's Environmental Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200129, China.4Department of Chinese Materia Medica, College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China.5Department of Developmental and Behavioral Pediatrics, Shanghai Institute of Pediatric Translational Medicine, Shanghai Children's Medical Center, Ministry of Education-Shanghai Key Laboratory of Children's Environmental Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200129, China; Departments of Anatomy and Embryology, Biochemistry and Molecular Cell Biology, Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Institute of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. Electronic address: wgli@shsmu.edu.cn.6Department of Developmental and Behavioral Pediatrics, Shanghai Institute of Pediatric Translational Medicine, Shanghai Children's Medical Center, Ministry of Education-Shanghai Key Laboratory of Children's Environmental Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200129, China. Electronic address: feili@shsmu.edu.cn.AbstractRhizoma Curcumae is a common Chinese dietary spice used in South Asia and China for thousands of years. As the main extract, Rhizoma Curcumae oil has attracted a great interest due to its newly raised therapeutic activities including its pharmacological effects upon central nervous system such as neuroprotection, cognitive enhancement, and anti-seizure efficacy; however the molecular mechanisms and the target identification remain to be established. Here we characterize an inhibitory effect of curcumol, a major bioactive component of Rhizoma Curcumae oil, on the excitability of hippocampal neurons in culture, the basal locomotor activity of freely moving animals, and the chemically induced seizure activity in vivo. Electrophysiological recording showed that acute application of curcumol significantly facilitated the γ-aminobutyric acid (GABA)-activated current in cultured mouse hippocampal neurons and in human embryonic kidney cells expressing α1- or α5-containing A type GABA (GABAA) receptors in a concentration-dependent manner. Measurement of tonic and miniature inhibitory postsynaptic GABAergic currents in hippocampal slices indicated that curcumol enhanced both forms of inhibition. In both pentylenetetrazole and kainate seizure models, curcumol suppressed epileptic activity in mice by prolonging the latency to clonic and tonic seizures and reducing the mortality as well as the susceptibility to seizure, presumably by facilitating the activation of GABAA receptors. Taken together, our results identified curcumol as a novel anti-seizure agent which inhibited neuronal excitability through enhancing GABAergic inhibition.
- Neuropharmacology.Neuropharmacology.2014 Jun;81:244-55. doi: 10.1016/j.neuropharm.2014.02.009. Epub 2014 Feb 21.
- Rhizoma Curcumae is a common Chinese dietary spice used in South Asia and China for thousands of years. As the main extract, Rhizoma Curcumae oil has attracted a great interest due to its newly raised therapeutic activities including its pharmacological effects upon central nervous system such as ne
- PMID 24565642
Japanese Journal
- Inhibitory Effects of Acorn Extract on Glutamate-Induced Calcium Signaling in Cultured Rat Hippocampal Neurons
- Lee Joonwoo,Hong Yi Jae,Yang Ji Seon [他]
- Biological & pharmaceutical bulletin 36(3), 331-338, 2013-03
- NAID 40019588137
- Self-Patterning of PC12 Cells on Micro-Structures of Protein-Modified Particles
- SUGIHARA Tatsuki,KANEKO Arata
- Journal of Solid Mechanics and Materials Engineering 7(2), 142-154, 2013
- … In this present study, using silica (SiO2) particles modified by specific protein, effects of particle-structure and protein-modification are investigated in a case of cultured rat pheochromocytoma (PC12) cells. … This indicates subsequent differentiation enables a desired network of neuron on the structure. …
- NAID 130003366852
- 結合強度推定に基づくニューロン種の分類:—ドーパミンニューロンを含む培養神経回路網の電気活動—
- 磯村 拓哉,武内 彬正,榛葉 健太,小谷 潔,神保 泰彦
- IEEJ Transactions on Electronics, Information and Systems 133(9), 1806-1813, 2013
- … Connection-strength estimation based on multi-neuron spike data enables us to estimate effective connectivity between neurons and is widely used for investigating information processing in neuronal networks. … We applied connection-strength estimation to categorize neuron types from multi-neuron spike data which were recorded from cultivated neuronal networks including dopamine ones. …
- NAID 130003363417
Related Links
- Cultured Neuron This is a single neuron which has been grown in a petri dish. The lump at the far end is the cell body. The flat part spread out at the closer end is the growth cone area, a part of the neuron that is trying to find the cell ...
- Detection and assessment of faces in neurite skeletons for use in the branch-count correction. (A) A cultured neuron demonstrating three types of simple neurite contacts: t, tip-to-neurite; d, tip-to-tip; x, neurite crossover. (B smeL ...
Related Pictures
★リンクテーブル★
| リンク元 | 「培養神経細胞」「培養ニューロン」 |
| 拡張検索 | 「primary cultured neuron」 |
| 関連記事 | 「neuro」「culture」 |
「培養神経細胞」
「培養ニューロン」
- 英
- cultured neuron
- 関
- ・養神経細胞
「primary cultured neuron」
「neuro」
- comb form.
「culture」
- n.