English Journal

Benders GA.
Methods in molecular biology (Clifton, N.J.).Methods Mol Biol.2012;852:165-80. doi: 10.1007/978-1-61779-564-0_13.
Many bacterial and archaeal genomes are of a similar size to molecules that have been cloned in the yeast Saccharomyces cerevisiae and thus might be clonable as single, circular episomes in this host. Yeast offers a variety of efficient tools for the manipulation and study of cloned DNA. One strateg
PMID 22328433

A new method for protein coexpression in Escherichia coli using two incompatible plasmids.

Yang W1, Zhang L, Lu Z, Tao W, Zhai Z.
Protein expression and purification.Protein Expr Purif.2001 Aug;22(3):472-8.
It is commonly believed that incompatible plasmids carrying the same replicon cannot coexist stably in one Escherichia coli cell. However, we found that two incompatible plasmids carrying different antibiotic resistance genes, if under the selection pressure of the two antibiotics, can coexist in E.
PMID 11483011

Transgenic rice plants expressing the ferredoxin-like protein (AP1) from sweet pepper show enhanced resistance to Xanthomonas oryzae pv. oryzae.

Tang K1, Sun X, Hu Q, Wu A, Lin C, Lin H, Twyman RM, Christou P, Feng T.
Plant science : an international journal of experimental plant biology.Plant Sci.2001 Apr;160(5):1035-1042.
We used particle bombardment to cotransform mature seed-derived rice callus (Oryza sativa L., ssp. japonica, cv. Eyi 105) with plasmids containing the linked marker genes gusA and hpt, and the ap1 gene encoding an amphipathic protein previously shown to delay the hypersensitive response induced in n
PMID 11297801