カルバモイルリン酸合成酵素
WordNet
- a salt of phosphoric acid (同)orthophosphate, inorganic_phosphate
- carbonated drink with fruit syrup and a little phosphoric acid
PrepTutorEJDIC
- 〈U〉リン酸塩 / 《複数形で》リン酸肥料
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English Journal
- Specific Discrimination of Three Pathogenic Salmonella enterica subsp. enterica Serotypes by carB-Based Oligonucleotide Microarray.
- Shin HH, Hwang BH, Seo JH, Cha HJ.Author information Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, South Korea.AbstractIt is important to rapidly and selectively detect and analyze pathogenic Salmonella enterica subsp. enterica in contaminated food to reduce the morbidity and mortality of Salmonella infection and to guarantee food safety. In the present work, we developed an oligonucleotide microarray containing duplicate specific capture probes based on the carB gene, which encodes the carbamoyl phosphate synthetase large subunit, as a competent biomarker evaluated by genetic analysis to selectively and efficiently detect and discriminate three S. enterica subsp. enterica serotypes: Choleraesuis, Enteritidis, and Typhimurium. Using the developed microarray system, three serotype targets were successfully analyzed in a range as low as 1.6 to 3.1 nM and were specifically discriminated from each other without nonspecific signals. In addition, the constructed microarray did not have cross-reactivity with other common pathogenic bacteria and even enabled the clear discrimination of the target Salmonella serotype from a bacterial mixture. Therefore, these results demonstrated that our novel carB-based oligonucleotide microarray can be used as an effective and specific detection system for S. enterica subsp. enterica serotypes.
- Applied and environmental microbiology.Appl Environ Microbiol.2014 Jan;80(1):366-73. doi: 10.1128/AEM.02978-13. Epub 2013 Nov 1.
- It is important to rapidly and selectively detect and analyze pathogenic Salmonella enterica subsp. enterica in contaminated food to reduce the morbidity and mortality of Salmonella infection and to guarantee food safety. In the present work, we developed an oligonucleotide microarray containing dup
- PMID 24185846
- De novo pyrimidine biosynthesis in the oomycete plant pathogen Phytophthora infestans.
- Bayona LG1, Garavito MF1, Lozano GL2, Vasquez JJ1, Myers K3, Fry WE3, Bernal A4, Zimmermann BH2, Restrepo S5.Author information 1Laboratory of Mycology and Plant Pathology, Universidad de los Andes, Carrera 1 # 18-10, Edificio J-205, Bogotá DC, Colombia; Grupo de Investigaciones en Bioquímica y Biología Molecular de Parásitos, Universidad de los Andes, Carrera 1 # 18-10, Edificio M-301, Bogotá DC, Colombia.2Grupo de Investigaciones en Bioquímica y Biología Molecular de Parásitos, Universidad de los Andes, Carrera 1 # 18-10, Edificio M-301, Bogotá DC, Colombia.3Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY, USA.4Laboratory of Mycology and Plant Pathology, Universidad de los Andes, Carrera 1 # 18-10, Edificio J-205, Bogotá DC, Colombia.5Laboratory of Mycology and Plant Pathology, Universidad de los Andes, Carrera 1 # 18-10, Edificio J-205, Bogotá DC, Colombia. Electronic address: srestrep@uniandes.edu.co.AbstractThe oomycete Phytophthora infestans, causal agent of the tomato and potato late blight, generates important economic and environmental losses worldwide. As current control strategies are becoming less effective, there is a need for studies on oomycete metabolism to help identify promising and more effective targets for chemical control. The pyrimidine pathways are attractive metabolic targets to combat tumors, virus and parasitic diseases but have not yet been studied in Phytophthora. Pyrimidines are involved in several critical cellular processes and play structural, metabolic and regulatory functions. Here, we used genomic and transcriptomic information to survey the pyrimidine metabolism during the P. infestans life cycle. After assessing the putative gene machinery for pyrimidine salvage and de novo synthesis, we inferred genealogies for each enzymatic domain in the latter pathway, which displayed a mosaic origin. The last two enzymes of the pathway, orotate phosphoribosyltransferase and orotidine-5-monophosphate decarboxylase, are fused in a multi-domain enzyme and are duplicated in some P. infestans strains. Two splice variants of the third gene (dihydroorotase) were identified, one of them encoding a premature stop codon generating a non-functional truncated protein. Relative expression profiles of pyrimidine biosynthesis genes were evaluated by qRT-PCR during infection in Solanum phureja. The third and fifth genes involved in this pathway showed high up-regulation during biotrophic stages and down-regulation during necrotrophy, whereas the uracil phosphoribosyl transferase gene involved in pyrimidine salvage showed the inverse behavior. These findings suggest the importance of de novo pyrimidine biosynthesis during the fast replicative early infection stages and highlight the dynamics of the metabolism associated with the hemibiotrophic life style of pathogen.
- Gene.Gene.2013 Dec 18. pii: S0378-1119(13)01670-3. doi: 10.1016/j.gene.2013.12.009. [Epub ahead of print]
- The oomycete Phytophthora infestans, causal agent of the tomato and potato late blight, generates important economic and environmental losses worldwide. As current control strategies are becoming less effective, there is a need for studies on oomycete metabolism to help identify promising and more e
- PMID 24361203
- Expression, purification, crystallization and preliminary X-ray diffraction analysis of the aspartate transcarbamoylase domain of human CAD.
- Ruiz-Ramos A, Lallous N, Grande-García A, Ramón-Maiques S.Author information Structural Bases of Genome Integrity Group, Structural Biology and Biocomputing Programme, Spanish National Cancer Research Centre (CNIO), Melchor Fernández Almagro 3, 28029 Madrid, Spain.AbstractAspartate transcarbamoylase (ATCase) catalyzes the synthesis of N-carbamoyl-L-aspartate from carbamoyl phosphate and aspartate in the second step of the de novo biosynthesis of pyrimidines. In prokaryotes, the first three activities of the pathway, namely carbamoyl phosphate synthetase (CPSase), ATCase and dihydroorotase (DHOase), are encoded as distinct proteins that function independently or in noncovalent association. In animals, CPSase, ATCase and DHOase are part of a 243 kDa multifunctional polypeptide named CAD. Up-regulation of CAD is essential for normal and tumour cell proliferation. Although the structures of numerous prokaryotic ATCases have been determined, there is no structural information about any eukaryotic ATCase. In fact, the only detailed structural information about CAD is that it self-assembles into hexamers and trimers through interactions of the ATCase domains. Here, the expression, purification and crystallization of the ATCase domain of human CAD is reported. The recombinant protein, which was expressed in bacteria and purified with good yield, formed homotrimers in solution. Crystallization experiments both in the absence and in the presence of the inhibitor PALA yielded small crystals that diffracted X-rays to 2.1 Å resolution using synchrotron radiation. The crystals appeared to belong to the hexagonal space group P6322, and Matthews coefficient calculation indicated the presence of one ATCase subunit per asymmetric unit, with a solvent content of 48%. However, analysis of the intensity statistics suggests a special case of the P21 lattice with pseudo-symmetry and possibly twinning.
- Acta crystallographica. Section F, Structural biology and crystallization communications.Acta Crystallogr Sect F Struct Biol Cryst Commun.2013 Dec;69(Pt 12):1425-30. doi: 10.1107/S1744309113031114. Epub 2013 Nov 29.
- Aspartate transcarbamoylase (ATCase) catalyzes the synthesis of N-carbamoyl-L-aspartate from carbamoyl phosphate and aspartate in the second step of the de novo biosynthesis of pyrimidines. In prokaryotes, the first three activities of the pathway, namely carbamoyl phosphate synthetase (CPSase), ATC
- PMID 24316846
Japanese Journal
- Little gene flow between domestic silkmoth Bombyx mori and its wild relative Bombyx mandarina in Japan, and possible artificial selection on the CAD gene of B. mori
- Yukuhiro Kenji,Sezutsu Hideki,Tamura Toshiki,Kosegawa Eiichi,Iwata Kazuya,Ajimura Masahiro,Gu Shi-Hong,Wang Min,Xia Qingyou,Mita Kazuei,Kiuchi Makoto
- Genes & Genetic Systems 87(5), 331-340, 2012
- … We analyzed PCR-amplified carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD) gene fragments from 146 Bombyx mori native strains and found extremely low levels of DNA polymorphism. …
- NAID 130003360984
- Effect of Conclevan on Endurance Capacity in Mice
- Ikarashi Nobutomo,Fukazawa Yoko,Toda Takahiro [他],Ishii Makoto,Ochiai Wataru,Usukura Masatoshi,Sugiyama Kiyoshi
- Biological and Pharmaceutical Bulletin 35(2), 231-238, 2012
- … The mRNA expression levels of the hepatic enzymes of the urea cycle (carbamoyl-phosphate synthetase, argininosuccinate synthetase, and arginase) and glutamine synthesis (glutamate dehydrogenase and glutamine synthetase) were significantly increased in the Conclevan administration group, compared to the swimming control group. …
- NAID 130001872298
- A case of carbamoyl phosphate synthetase 1 deficiency presenting symptoms at one month of age
- ONO Hiroaki,SUTO Tetsushi,KINOSHITA Yoshihisa,SAKANO Takashi,FURUE Takeki,OHTA Toshiyuki
- Brain & development 31(10), 779-781, 2009-11-01
- NAID 10026413113
Related Links
- Carbamoyl phosphate synthetase (CPS) deficiency is a urea cycle defect that results from a deficiency in an enzyme that mediates the normal path for incorporation of ammonia. CPS is derived from catabolism of amino ...
- Carbamoyl phosphate synthetase I deficiency is an inherited disorder that causes ammonia to accumulate in the blood (hyperammonemia). Ammonia, which is formed when proteins are broken down in the body, is toxic ...
★リンクテーブル★
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- 英
- carbamoyl-phosphate synthetase、carbamoyl-phosphate synthase, carbamyl phosphate synthetase CPS
- 関
- カルバモイルリン酸シンテターゼ、カルバモイルリン酸合成酵素I、カルバモイルリン酸シンターゼ
[show details]
carbamylphosphate synthetase : 約 80,400 件
carbamoylphosphate synthetase : 約 16,200 件
carbamylphosphate synthetase : 30 件
carbamoylphosphate synthetase : 67 件
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カルバモイルリン酸合成酵素、カルバモイルリン酸シンターゼ
- 関
- carbamoyl-phosphate synthetase、carbamoyl-phosphate synthetase I
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- 英
- carbamoyl-phosphate synthetase
- 関
- カルバモイルリン酸合成酵素
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カルバモイルリン酸合成酵素欠損症
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- 関
- inorganic phosphate、orthophosphate、orthophosphoric acid、phospho、phosphoester、phosphoric、phosphoric acid、phosphoric acid ester、phosphorus
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- 関
- ligase、synthase