WordNet

a general term for the research activity that creates a copy of some biological entity (a gene or organism or cell)

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Cloning and characterization of the ionotropic GABA receptor subunit ρ1 from pig (Sus scrofa).

Reyes-Ruiz JM1, Limon A2, Miledi R2.
Neuroscience letters.Neurosci Lett.2014 Jan 13;558:78-81. doi: 10.1016/j.neulet.2013.11.003. Epub 2013 Nov 14.
Since human and pig eyes have remarkably anatomical and physiological similitudes swine models have been broadly used for functional studies and therapeutic research. Recently, a GABAρ-mediated relaxation of retinal vascularity suggested that GABAρ signaling may be used to improve retinal blood fl
PMID 24239647

Feng H, Xia X, Fan X1, Xu G, Miller AJ.
Plant methods.Plant Methods.2013 Dec 20;9(1):48. doi: 10.1186/1746-4811-9-48.
BACKGROUND: Rapid improvements in DNA synthesis technology are revolutionizing gene cloning and the characterization of their encoded proteins. Xenopus laevis oocytes are a commonly used heterologous system for the expression and functional characterization of membrane proteins. For many plant prote
PMID 24359672

Generation and validation of a zebrafish model of EAST (epilepsy, ataxia, sensorineural deafness and tubulopathy) syndrome.

Mahmood F1, Mozere M, Zdebik AA, Stanescu HC, Tobin J, Beales PL, Kleta R, Bockenhauer D, Russell C.
Disease models & mechanisms.Dis Model Mech.2013 May;6(3):652-60. doi: 10.1242/dmm.009480. Epub 2013 Feb 14.
Recessive mutations in KCNJ10, which encodes an inwardly rectifying potassium channel, were recently identified as the cause of EAST syndrome, a severe and disabling multi-organ disorder consisting of epilepsy, ataxia, sensorineural deafness and tubulopathy that becomes clinically apparent with seiz
PMID 23471908

Molecular characterization and histochemical demonstration of salmon olfactory marker protein in the olfactory epithelium of lacustrine sockeye salmon (Oncorhynchus nerka)

Kudo H.,Doi Y.,Ueda H.,Kaeriyama M.
Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology 154(1), 142-150, 2009-09
… By in situ hybridization using a digoxigeninlabeled salmon OMP cRNA probe, signals for salmon OMP mRNA were observed preferentially in the perinuclear regions of the ORNs. … The present study is the first to describe cDNA cloning of OMP in salmon olfactory epithelium, and indicate that OMP is a useful molecular marker for the detection of the ORNs in Pacific salmon. …
NAID 120001425535

Cloning and functional characterization of a novel up-regulator, cartregulin, of carnitine transporter, OCTN2

Nagai Kazuhiko,Takikawa Osamu,Kawakami Naoko,Fukao Mitsuhiro,Soma Takamitsu,Oda Atsushi,Nishiya Tadashi,Hayashi Misato,Lu Lingyun,Nakano Manabu,Kajita Emi,Fujita Hiroyoshi,Miwa Soichi
Archives of Biochemistry and Biophysics 452(1), 29-37, 2006-08-01
… When OCTN2 was expressed in Xenopus oocytes by injection of its cRNA, its transport activity was enhanced by co-expression of the novel protein. …
NAID 120000960814

An mRNA amplification procedure with directional cDNA cloning and strand-specific cRNA synthesis for comprehensive gene expression analysis