bacterial inhibition assay

同

BIA法

WordNet

1. a quantitative or qualitative test of a substance (especially an ore or a drug) to determine its components; frequently used to test for the presence or concentration of infectious agents or antibodies etc.
2. analyze (chemical substances)
3. a substance that is undergoing an analysis of its components
4. an appraisal of the state of affairs; "they made an assay of the contents"; "a check on its dependability under stress" (同)check
5. a written report of the results of an analysis of the composition of some substance
6. (physiology) the process whereby nerves can retard or prevent the functioning of an organ or part; "the inhibition of the heart by the vagus nerve"
7. (psychology) the conscious exclusion of unacceptable thoughts or desires (同)suppression
8. the quality of being inhibited
9. (microbiology) single-celled or noncellular spherical or spiral or rod-shaped organisms lacking chlorophyll that reproduce by fission; important as pathogens and for biochemical properties; taxonomy is difficult; often considered to be plants (同)bacterium
10. hardy and sure-footed animal smaller and with longer ears than the horse
11. a pompous fool
12. relating to or caused by bacteria; "bacterial infection"

PrepTutorEJDIC

1. (金属の品質・鉱石の金属含有量・薬品の)分析,試金 / 試金物,分析物 / …'を'試金する,分析する
2. 禁止; 抑制; （心理学で）抑制; （化学で）（反応の）阻害, 抑制
3. 『バクテリア』,細菌
4. ロバ / ばか者
5. 《米俗》しり,けつ(《英俗》arse)
6. バクテリアの,細菌の

Wikipedia preview

出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2015/06/08 14:11:04」(JST)

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[Wiki en表示]

Haemophilus influenzae requires X and V factors for growth. In this culture haemophilus has only grown around the paper disc that has been impregnated with X and V factors. There is no bacterial growth around the discs that only contain either X or V factor.

A bacterial inhibition assay is an assay that uses bacterial colony growth as a semi quantitative indicator to measure the concentration of a product in a sample.

Example: classical Guthrie test

The Guthrie bacterial inhibition assay was a semiquantitative assay designed to detect elevated blood levels of the amino acid phenylalanine, using the ability of phenylalanine to facilitate bacterial growth in a culture medium with an inhibitor.

A drop of blood is usually obtained by pricking the heel of a newborn infant and is collected on a piece of filter paper. A small disk of the filter paper is punched out and placed on an agar gel plate containing Bacillus subtilis and B-2-thienylalanine. Each gel holds 60-80 disks. The agar gel is able to support bacterial growth but the B-2-thienylalanine inhibits bacterial growth. However, in the presence of extra phenylalanine leached from the impregnated filter paper disk, the inhibition is overcome and the bacteria grow. Within a day the bacterial growth surrounding the paper disk is visible. The amount of growth, measured as the diameter of the colony, is roughly proportional to the amount of phenylalanine in the serum. The result is read by comparing the diameter of each sample disk's colony to the colonies of a series of reference disks with standard phenylalanine content included on each large plate.

The Guthrie bacterial inhibition assay is sensitive enough to detect serum phenylalanine levels of 180-240 μmol/L (3–4 mg/dL). In healthy normal people, phenylalanine levels are usually under 120 μmol/L.

The test has been widely used throughout North America and Europe as one of the core newborn screening tests since the late 1960s. The classical Guthrie bacterial inhibition assay is gradually being replaced in many areas by newer techniques such as tandem mass spectrometry that can detect a wider variety of congenital diseases.

See also: Neonatal heel prick

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Japanese Journal

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• Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy 18(1), 22-29, 2012-02-01
• … We have previously demonstrated that the steroid receptor antagonist mifepristone (RU486) causes growth inhibition of Chlamydophila pneumoniae by binding to and subsequently destroying the bacteria during their normal developmental cycle in epithelial HEp-2 cells. … Assessment of bacterial growth modification, the number of infectious progenies, the formation of inclusions, and the expressions of the C. …
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Related Links

• Guthrie's bacterial inhibition assay - definition of Guthrie's ...

Guthrie's bacterial inhibition assay (GBIA) [guth′rēz] a screening for phenylketonuria (PKU) used to detect the abnormal presence of phenylalanine metabolites in the blood. A small amount of blood is obtained and placed in a medium ...

• Laboratory procedure of bacterial inhibition assay

BACTERIAL INHIBITION ASSAY By: MARY JEAN D. SOMCIO, RMT ... Laboratory procedure of bacterial inhibition assay Presentation Transcript BACTERIAL INHIBITION ASSAY By: MARY JEAN D. SOMCIO, RMT ...

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★リンクテーブル★

リンク元	「ガスリー法」
関連記事	「assay」「inhibition」「bacterial」

「ガスリー法」

　　[★]

英

Guthrie test

同

ガスリー試験 Guthrie試験 Guthrie法、BIA法 bacterial inhibition assay

[show details]

ガスリー試験 : 25 件
ガスリー法 : 約 798 件
Guthrie試験 : 7 件
Guthrie法 : 95 件

-Guthrie法

「assay」

　　[★]

• n.

• アッセイ、アッセイ法、測定法、試験法、検定法、検定、分析

• v.

• 検定する、定量する、アッセイする

関

anal、analyse、analyses、analysis、analytical、analyze、determination、determine、dissect、method、method for measurement、-metry、quantitative determination、statistical method、test

　 　 　 　 　 　

「inhibition」

　　[★]

• n.

• 1.抑止、抑制、阻止、禁止
• 2.抑止(阻止)するもの
• 3.(心理学)(衝動などの)抑制
• 4.(化)(化学反応の)抑制
• 5.(生物)(酵素の働きなどの)抑制、阻害

　 　 　 　

「bacterial」

　　[★]

• adj.

• 細菌性の、細菌の、細菌的な

関

bacteria、bacterially、bacterio、bacterium