bFGF

出典: meddic

basic FGF, basic fibroblast growth factor
FGF-2 塩基性線維芽細胞成長因子
FGF fibroblast growth factor
  • 癌における血管新生に関与している (同:VEGF。対:angiostatin, endostatin, vasculostatin)
  • 細胞間マトリックスに蓄えらているbFGFはプロテアーゼによって放出される (BPT.201)
  • mitogenである:mesenchymall, neural, and epithelial organ



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出典(authority):フリー百科事典『ウィキペディア(Wikipedia)』「2013/06/03 16:10:34」(JST)

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英文文献

  • Neurogenesis of Neural Crest-Derived Periodontal Ligament Stem Cells by EGF and bFGF.
  • Fortino VR, Chen RS, Pelaez D, Cheung HS.Author information Department of Biomedical Engineering, College of Engineering, University of Miami, Coral Gables, Florida.AbstractNeuroregenerative medicine is an ever-growing field in which regeneration of lost cells/tissues due to a neurodegenerative disease is the ultimate goal. With the scarcity of available replacement alternatives, stem cells provide an attractive source for regenerating neural tissue. While many stem cell sources exist, including: mesenchymal stem cells, embryonic stem cells, and induced pluripotent stem cells, the limited cellular potency, technical difficulties, and ethical considerations associated with these make finding alternate sources a desirable goal. Periodontal ligament stem cells (PDLSCs) derived from the neural crest were induced into neural-like cells using a combination of epidermal growth factor, and basic fibroblast growth factor. Morphological changes were evident in our treated group, seen under both light microscopy and scanning electron microscopy. A statistically significant increase in the expression of neuron-specific β-tubulin III and the neural stem/progenitor cell marker nestin, along with positive immunohistochemical staining for glial fibrillary acidic protein, demonstrated the success of our treatment in inducing both neuronal and glial phenotypes. Positive staining for synaptophysin demonstrated neural connections and electrophysiological recordings indicated that when subjected to whole-cell patch clamping, our treated cells displayed inward currents conducted through voltage-gated sodium (Na(+) ) channels. Taken together, our results indicate the success of our treatment in inducing PDLSCs to neural-like cells. The ease of sourcing and expansion, their embryologic neural crest origin, and the lack of ethical implications in their use make PDLSCs an attractive source for use in neuroregenerative medicine. J. Cell. Physiol. 229: 479-488, 2014. © 2013 Wiley Periodicals, Inc.
  • Journal of cellular physiology.J Cell Physiol.2014 Apr;229(4):479-88. doi: 10.1002/jcp.24468.
  • Neuroregenerative medicine is an ever-growing field in which regeneration of lost cells/tissues due to a neurodegenerative disease is the ultimate goal. With the scarcity of available replacement alternatives, stem cells provide an attractive source for regenerating neural tissue. While many stem ce
  • PMID 24105823
  • Bone marrow-derived myofibroblasts promote colon tumorigenesis through the IL-6/JAK2/STAT3 pathway.
  • Zhu L1, Cheng X2, Ding Y2, Shi J3, Jin H3, Wang H3, Wu Y2, Ye J4, Lu Y4, Wang TC5, Yang CS3, Tu SP6.Author information 1Department of Gastroenterology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China; Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA.2Department of Gastroenterology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.3Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA.4Emergency Medicine, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.5Department of Medicine, College of Physicians & Surgeons, Columbia University, New York, NY 10032, USA.6Department of Gastroenterology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China; Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA. Electronic address: tushuiping@yahoo.com.AbstractBone marrow-derived myofibroblasts (BMFs) have been shown to promote tumor growth. Here, we found that BMFs or BMF conditioned medium (BMF-CM) induced cancer stem cell-like sphere formation of colon cancer cells. The co-cultured BMFs, but not co-cultured cancer cells, expressed higher levels of IL-6 than BMFs or cancer cells cultured alone. Anti-mouse IL-6 neutralizing antibody, JAK2 inhibitors and STAT3 knockdown in mouse cancer cells reduced BMF- and BMF-CM-induced sphere formation of colon cancer cells. When co-injected, BMFs significantly enhanced tumorigenesis of colon cancer cells in mice. Our results demonstrate that BMFs promote tumorigenesis via the activation of the IL-6/JAK2/STAT3 pathway.
  • Cancer letters.Cancer Lett.2014 Feb 1;343(1):80-9. doi: 10.1016/j.canlet.2013.09.017. Epub 2013 Oct 18.
  • Bone marrow-derived myofibroblasts (BMFs) have been shown to promote tumor growth. Here, we found that BMFs or BMF conditioned medium (BMF-CM) induced cancer stem cell-like sphere formation of colon cancer cells. The co-cultured BMFs, but not co-cultured cancer cells, expressed higher levels of IL-6
  • PMID 24145153
  • Regulation of human mesenchymal stem cells differentiation into chondrocytes in extracellular matrix-based hydrogel scaffolds.
  • Du M1, Liang H1, Mou C1, Li X1, Sun J1, Zhuang Y1, Xiao Z2, Chen B2, Dai J3.Author information 1Division of Nanobiomedicine Research and Suzhou Key Laboratory of Nanobiomedical Characterization, Suzhou Institute of Nano-Tech and Nano-Bionics (SINANO), Chinese Academy of Sciences (CAS), 398 Ruoshui Road, Suzhou Dushu Lake Science and Education Innovation District (SEID), Suzhou Industrial Park (SIP), Suzhou 215123, PR China.2State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences (CAS), Beijing 100190, PR China.3Division of Nanobiomedicine Research and Suzhou Key Laboratory of Nanobiomedical Characterization, Suzhou Institute of Nano-Tech and Nano-Bionics (SINANO), Chinese Academy of Sciences (CAS), 398 Ruoshui Road, Suzhou Dushu Lake Science and Education Innovation District (SEID), Suzhou Industrial Park (SIP), Suzhou 215123, PR China; State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences (CAS), Beijing 100190, PR China. Electronic address: jwdai@genetics.ac.cn.AbstractTo induce human mesenchymal stem cells (hMSCs) to differentiate into chondrocytes in three-dimensional (3D) microenvironments, we developed porous hydrogel scaffolds using the cartilage extracellular matrix (ECM) components of chondroitin sulfate (CS) and collagen (COL). The turbidity and viscosity experiments indicated hydrogel could form through pH-triggered co-precipitation when pH=2-3. Enzyme-linked immunosorbent assay (ELISA) confirmed the hydrogel scaffolds could controllably release growth factors as envisaged. Transforming growth factor-β (TGF-β) was released to stimulate hMSCs differentiation into chondrocytes; and then collagen binding domain-basic fibroblast growth factor (CBD-bFGF) was released to improve the differentiation and preserve the chondrocyte phenotype. In in vitro cell culture experiments, the differentiation processes were compared in different microenvironments: 2D culture in culture plate as control, 3D culture in the fabricated scaffolds without growth factors (CC), the samples with CBD-bFGF (CC-C), the samples with TGF-β (CC-T), the samples with CBD-bFGF/TGF-β (CC-CT). Real-time polymerase chain reaction (RT-PCR) revealed the hMSC marker genes of CD44 and CD105 decreased; at the same time the chondrocyte marker genes of collagen type II and aggrecan increased, especially in the CC-CT sample. Immunostaining results further confirmed the hMSC marker protein of CD 44 disappeared and the chondrocyte marker protein of collagen type II emerged over time in the CC-CT sample. These results imply the ECM-based hydrogel scaffolds with growth factors can supply suitable 3D cell niches for hMSCs differentiation into chondrocytes and the differentiation process can be regulated by the controllably released growth factors.
  • Colloids and surfaces. B, Biointerfaces.Colloids Surf B Biointerfaces.2014 Feb 1;114:316-23. doi: 10.1016/j.colsurfb.2013.10.001. Epub 2013 Oct 24.
  • To induce human mesenchymal stem cells (hMSCs) to differentiate into chondrocytes in three-dimensional (3D) microenvironments, we developed porous hydrogel scaffolds using the cartilage extracellular matrix (ECM) components of chondroitin sulfate (CS) and collagen (COL). The turbidity and viscosity
  • PMID 24231133
  • Valproic acid inhibits tumor angiogenesis in mice transplanted with Kasumi‑1 leukemia cells.
  • Zhang ZH1, Hao CL1, Liu P2, Tian X3, Wang LH1, Zhao L1, Zhu CM1.Author information 1Affiliated Hospital of Chengde Medical College, Chengde, Hebei 067000, P.R. China.2The First Hospital of Shijiazhuang City, Shijiazhuang, Hebei 050000, P.R. China.3Chinese PLA 89 Hospital, Weifang, Shandong 261000, P.R. China.AbstractHistone deacetylase (HDAC) inhibitors have been reported to inhibit tumor angiogenesis via the downregulation of angiogenic factors. Our previous in vitro studies demonstrated that valproic acid (VPA) exerted antitumor effects on Kasumi‑1 cells, which are human acute myeloid leukemia cells with an 8;21 chromosome translocation. In the present study, the effects of VPA on tumor angiogenesis were investigated in mice transplanted with Kasumi‑1 cells. Semi‑quantitative reverse transcription‑polymerase chain reaction, western blotting and immunohistochemistry were used to detect the expression of vascular endothelial growth factor (VEGF), VEGF receptor (VEGFR2) and basic fibroblast growth factor (bFGF). The tumor microvessel density was measured following staining with an anti‑CD34 antibody. Chromatin immunoprecipitation was used to study the effect of VPA‑induced histone hyperacetylation on VEGF transcription. An intraperitoneal injection of VPA inhibited tumor growth and angiogenesis in mice transplanted with Kasumi‑1 cells. The mRNA and protein expression of VEGF, VEGFR2 and bFGF were inhibited by VPA treatment. In addition, VPA downregulated HDAC, increased histone H3 acetylation and enhanced the accumulation of hyperacetylated histone H3 on the VEGF promoters. The findings of the present study indicate that VPA, an HDAC inhibitor, exerts an antileukemic effect through an anti‑angiogenesis mechanism. In conclusion, the mechanism underlying VPA‑induced anti‑angiogenesis is associated with the suppression of angiogenic factors and their receptors. VPA may increase the accumulation of acetylated histones on the VEGF promoters, which possibly contributes to the regulation of angiogenic factors.
  • Molecular medicine reports.Mol Med Rep.2014 Feb;9(2):443-9. doi: 10.3892/mmr.2013.1834. Epub 2013 Nov 28.
  • Histone deacetylase (HDAC) inhibitors have been reported to inhibit tumor angiogenesis via the downregulation of angiogenic factors. Our previous in vitro studies demonstrated that valproic acid (VPA) exerted antitumor effects on Kasumi‑1 cells, which are human acute myeloid leukemia cells with a
  • PMID 24297248

和文文献

  • bFGF徐放性材料を用いた骨再生モデルにおける骨再生と血管新生の検討(岩手医科大学学位審査報告)
  • 大橋 祐生
  • 岩手医科大学歯学雑誌 36(2), 124-125, 2011-08-19
  • NAID 110008733448
  • P-68 水酸化ナトリウム水溶液を用いたラット食道狭窄モデルにおけるbFGFの治療効果(研究・その他2,ポスターセッション,第48回日本小児外科学会学術集会)
  • 大片 祐一,久松 千恵子,西島 栄治
  • 日本小児外科学会雑誌 47(4), 621, 2011-07-05
  • NAID 110008735190
  • bFGF徐放性材料を用いた骨再生モデルにおける骨再生と血管新生の検討
  • 大橋 祐生,藤村 朗
  • 岩手医科大学歯学雑誌 36(1), 19-34, 2011-05-10
  • … 防御の面において重要であると考えられる.本研究は,bFGF徐放システムによる骨再生モデルを用いて,マイクロフォーカスCTにより同一個体の経時的な骨再生の経過を観察し,連続組織標本を作製することで,骨再生と血管新生の関係を明らかにすることを目的とした.実験方法は,10週齢のWistar系ラットの頭頂骨に直径7mmの骨欠損を形成し,実験群にはbFGF 10μg含有酸性ゼラチンディスクを埋入した.また,対照 …
  • NAID 110008752157

関連リンク

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FGF(細胞増殖因子)注入|渋谷の美容整形ならベル美容外科クリニックへ。アンチエイジング、ほうれい線、しわ、シミなどさまざまなお悩みのご相談を承っております。安心安全で適切な治療を行ってまいります。無料オンライン ...
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★リンクテーブル★
先読みFGF
リンク元成長因子」「線維芽細胞増殖因子」「血管新生

FGF」

  [★] 線維芽細胞増殖因子 fibroblast growth factor


成長因子」

  [★]

成長因子 growth factor
成長促進因子 growth-promoting substance、発育因子増殖因子
[show details]


血管新生

angiogenic factor

angiostasis



線維芽細胞増殖因子」

  [★]

fibroblast growth factor, FGF
線維芽細胞成長因子, ヘパリン結合性増殖因子 heparin-binding growth factor HBGF
bFGFFGFファミリー


種類


血管新生」

  [★]

angiogenesis
二次血管発生
脈管形成 vasculogenesis
  • 既存の血管から芽が出て新たな血管が形成される

==血管新生促進

血管新生抑制


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