関: ASGP-R、ASGPR

WordNet

a cellular structure that is postulated to exist in order to mediate between a chemical agent that acts on nervous tissue and the physiological response

PrepTutorEJDIC

=sense organ / 受信装置

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2015/07/08 04:46:19」(JST)

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The asialoglycoprotein receptors are lectins which bind asialoglycoprotein, glycoproteins from which a sialic acid has been removed to expose galactose residues. The receptors, which are located on liver cells, remove the target glycoproteins from circulation.

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Asialoglycoprotein receptor at the US National Library of Medicine Medical Subject Headings (MeSH)

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English Journal

Spheroid culture for enhanced differentiation of human embryonic stem cells to hepatocyte-like cells.

Subramanian K, Owens DJ, Raju R, Firpo M, O'Brien TD, Verfaillie CM, Hu WS.Author information 1 Department of Chemical Engineering and Materials Science, University of Minnesota , Minneapolis, Minnesota.AbstractStem cell-derived hepatocyte-like cells hold great potential for the treatment of liver disease and for drug toxicity screening. The success of these applications hinges on the generation of differentiated cells with high liver specific activities. Many protocols have been developed to guide human embryonic stem cells (hESCs) to differentiate to the hepatic lineage. Here we report cultivation of hESCs as three-dimensional aggregates that enhances their differentiation to hepatocyte-like cells. Differentiation was first carried out in monolayer culture for 20 days. Subsequently cells were allowed to self-aggregate into spheroids. Significantly higher expression of liver-specific transcripts and proteins, including Albumin, phosphoenolpyruvate carboxykinase, and asialoglycoprotein receptor 1 was observed. The differentiated phenotype was sustained for more than 2 weeks in the three-dimensional spheroid culture system, significantly longer than in monolayer culture. Cells in spheroids exhibit morphological and ultrastructural characteristics of primary hepatocytes by scanning and transmission electron microscopy in addition to mature functions, such as biliary excretion of metabolic products and cytochrome P450 activities. This three-dimensional spheroid culture system may be appropriate for generating high quality, functional hepatocyte-like cells from ESCs.
Stem cells and development.Stem Cells Dev.2014 Jan 15;23(2):124-31. doi: 10.1089/scd.2013.0097. Epub 2013 Oct 22.
Stem cell-derived hepatocyte-like cells hold great potential for the treatment of liver disease and for drug toxicity screening. The success of these applications hinges on the generation of differentiated cells with high liver specific activities. Many protocols have been developed to guide human e
PMID 24020366

In Vitro and in Vivo Evaluation of Pectin-Based Nanoparticles for Hepatocellular Carcinoma Drug Chemotherapy.

Yu CY, Wang YM, Li NM, Liu GS, Yang S, Tang GT, He DX, Tan XW, Wei H.Author information Institute of Pharmacy & Pharmacology, Department of Pharmacy, University of South China , Hengyang 421001, China.AbstractThe fabrication and evaluation of a natural pectin-based drug delivery system are reported in this study. The drug delivery system displays specific active targeting ability to hepatocellular carcinoma due to the presence of excess galactose residues in the polymer structure as the natural targeting ligands. The system was prepared under very mild conditions in an aqueous medium containing Ca2+ and CO32- ions, generating uniform pectin-based nanoparticles with an average diameter of 300 nm, and the drug-loading content of anticancer drug 5-fluorouracil (5-FU) is around 24.8%. Cytotoxicity study of the 5-FU-loaded nanoparticles (5-FU-NPs) in HepG2 and A549 cell lines demonstrated their greater potency in killing cancer cells with overexpressed asialoglycoprotein receptor (ASGPR) on the cell surface, compared to that of the free drug. Pharmacokinetics study using Sprague-Dawley (SD) rats further confirmed that the drug-loaded nanoparticles showed a much longer half-life in the circulation fluids than the free drug. Tissue distribution was investigated on Kunming mice, and the results also demonstrated that the 5-FU-NPs has a long circulation effect. Taken together, the pectin-based drug delivery systems exhibit size-induced prolonged circulation as well as ASGP receptor-mediated targeting ability to cancer cell lines; therefore, it is a promising platform for the treatment of hepatocellular carcinoma.
Molecular pharmaceutics.Mol Pharm.2014 Jan 9. [Epub ahead of print]
The fabrication and evaluation of a natural pectin-based drug delivery system are reported in this study. The drug delivery system displays specific active targeting ability to hepatocellular carcinoma due to the presence of excess galactose residues in the polymer structure as the natural targeting
PMID 24383625

In vivo hepatocyte MR imaging using lactose functionalized magnetoliposomes.

Ketkar-Atre A, Struys T, Dresselaers T, Hodenius M, Mannaerts I, Ni Y, Lambrichts I, Van Grunsven LA, De Cuyper M, Himmelreich U.Author information Biomedical MRI/MoSAIC, Department of Imaging and Pathology, Biomedical Sciences Group, Katholieke Universiteit Leuven, Herestraat 49, B3000 Leuven, Belgium.AbstractThe aim of this study was to assess a novel lactose functionalized magnetoliposomes (MLs) as an MR contrast agent to target hepatocytes as well as to evaluate the targeting ability of MLs for in vivo applications. In the present work, 17 nm sized iron oxide cores functionalized with anionic MLs bearing lactose moieties were used for targeting the asialoglycoprotein receptor (ASGP-r), which is highly expressed in hepatocytes. Non-functionalized anionic MLs were tested as negative controls. The size distribution of lactose and anionic MLs was determined by transmission electron microscopy (TEM) and dynamic light scattering (DLS). After intravenous administration of both MLs, contrast enhancement in the liver was observed by magnetic resonance imaging (MRI). Label retention was monitored non-invasively by MRI and validated with Prussian blue staining and TEM for up to eight days post MLs administration. Although the MRI signal intensity did not show significant differences between functionalized and non-functionalized particles, iron-specific Prussian blue staining and TEM analysis confirmed the uptake of lactose MLs mainly in hepatocytes. In contrast, non-functionalized anionic MLs were mainly taken up by Kupffer and sinusoidal cells. Target specificity was further confirmed by high-resolution MR imaging of phantoms containing isolated hepatocytes, Kupffer cell (KCs) and hepatic stellate cells (HSCs) fractions. Hypointense signal was observed for hepatocytes isolated from animals which received lactose MLs but not from animals which received anionic MLs. These data demonstrate that galactose-functionalized MLs can be used as a hepatocyte targeting MR contrast agent to potentially aid in the diagnosis of hepatic diseases if the non-specific uptake by KCs is taken into account.
Biomaterials.Biomaterials.2014 Jan;35(3):1015-24. doi: 10.1016/j.biomaterials.2013.10.029. Epub 2013 Nov 5.
The aim of this study was to assess a novel lactose functionalized magnetoliposomes (MLs) as an MR contrast agent to target hepatocytes as well as to evaluate the targeting ability of MLs for in vivo applications. In the present work, 17 nm sized iron oxide cores functionalized with anionic MLs bear
PMID 24210051

Japanese Journal

Galactose-Modified Cationic Liposomes as a Liver-Targeting Delivery System for Small Interfering RNA

Biological and Pharmaceutical Bulletin 34(8), 1338-1342, 2011
NAID 130000936610

siRNAのDDS : シクロデキストリン/デンドリマー結合体

Drug delivery system 25(6), 598-606, 2010-11-25
NAID 10027642911

Preoperative estimation of asialoglycoprotein receptor expression in the remnant liver from CT/^<99m>Tc-GSA SPECT fusion images correlates well with postoperative liver function parameters

Journal of hepato-biliary-pancreatic sciences 17(5), 673-681, 2010-09-01
NAID 10027663484

★リンクテーブル★

リンク元	「アシアロ糖タンパク質受容体」「ASGP-R」「アシアロ糖タンパク質レセプター」「アシアロ糖蛋白質受容体」
拡張検索	「antibody toasialoglycoprotein receptor」
関連記事	「asialoglycoprotein」