| N-Acetylglutamate synthase |
| Identifiers |
| Symbol |
NAGS |
| Entrez |
162417 |
| HUGO |
17996 |
| OMIM |
608300 |
| RefSeq |
NM_153006 |
| UniProt |
Q8N159 |
| Other data |
| EC number |
2.3.1.1 |
| Locus |
Chr. 17 q21.31 |
N-acetylglutamate synthase (NAGS) is an enzyme that catalyses the production of N-Acetylglutamate (NAG) from glutamate and acetyl-CoA.
Put simply NAGS catalyzes the following reaction:
- acetyl-CoA + L-glutamate → CoA + N-acetyl-L-glutamate
NAGS, a member of the N-acetyltransferase family of enzymes, is present in both prokaryotes and eukaryotes, although its role and structure differ widely depending on the species. NAG can be used in the production of ornithine and arginine, two important amino acids, or as an allosteric cofactor for carbamoyl phosphate synthase (CPS1). In mammals, NAGS is expressed primarily in the liver and small intestine, and is localized to the mitochondrial matrix.[1]
Overall reaction scheme for N-acetylglutamate (NAG) synthesis via N-acetylglutamate synthase (NAGS)
Contents
- 1 Biological Function
- 2 Mechanism
- 3 Clinical significance
- 4 References
- 5 External links
Biological Function
Most prokaryotes (bacteria) and lower eukaryotes (fungi, green algae, plants, etc.) produce NAG through orinithine acetyltransferase (OAT), which is part of a ‘cyclic’ ornithine production pathway. NAGS is therefore used in a supportive role, replenishing NAG reserves as required. In some plants and bacteria, however, NAGS catalyzes the first step in a ‘linear’ arginine production pathway.[2]
The protein sequences of NAGS between prokaryotes, lower eukaryotes and higher eukaryotes have shown a remarkable lack of similarity. Sequence identity between prokaryotic and eukaryotic NAGS is largely <30%,[3] while sequence identity between lower and higher eukaryotes is ~20%.[4]
Enzyme activity of NAGS is modulated by L-arginine, which acts as an inhibitor in plant and bacterial NAGS, but an effector in vertebrates.[5][6] While the role of arginine as an inhibitor of NAG in ornithine and arginine synthesis is well understood, there is some controversy as to the role of NAG in the urea cycle.[7][8] The currently accepted role of NAG in vertebrates is as an essential allosteric cofactor for CPS1, and therefore it acts as the primary controller of flux through the urea cycle. In this role, feedback regulation from arginine would act to signal NAGS that ammonia is plentiful within the cell, and needs to be removed, accelerating NAGS function. As it stands, the evolutionary journey of NAGS from essential synthetic enzyme to primary urea cycle controller is yet to be fully understood.[9]
Mechanism
A simplified reaction mechanism for N-Acetylglutamate synthase (NAGS)
Two mechanisms for N-acetyltransferase function have been proposed: a two-step, ping-pong mechanism involving transfer of the relevant acetyl group to an activated cysteine residue[10] and a one-step mechanism through direct attack of the amino nitrogen on the carbonyl group.[11] Studies conducted using NAGS derived from Neissria gonohorreae suggest that NAGS proceeds through the previously described one-step mechanism.[12] In this proposal, the carbonyl group of acetyl-CoA is attacked directly by the α-amino nitrogen of glutamate. This mechanism is supported by the activation of the carbonyl through hydrogen bond polarization, as well as the absence of a suitable cysteine within the active site to act as an intermediate acceptor of the acetyl group.[13][14]
Clinical significance
Inactivity of NAGS results in N-acetylglutamate synthase deficiency, a form of hyperammonemia.[15] In many vertebrates, N-acetylglutamate is an essential allosteric cofactor of CPS1, the enzyme that catalyzes the first step of the urea cycle.[16] Without NAG stimulation, CPS1 cannot convert ammonia to carbamoyl phosphate, resulting in toxic ammonia accumulation.[17] Carbamoyl glutamate has shown promise as a possible treatment for NAGS deficiency.[15] This is suspected to be a result of the structural similarities between NAG and carabamoyl glutamate, which allows carbamoyl glutamate to act as an effective agonist for CPS1.[14]
References
- ^ A.J Meijer, C Lof, I.C Ramos, A.J Verhoeven, "Control of ureagenesis", Eur. J. Biochem., 148 (1985), pp. 189–196
- ^ Cunin, R., Glansdorff, N., Pierard, A. and Stalon, V. (1986) Biosynthesis and metabolism of arginine in bacteria. Microbiol. Rev. 50, 314–352
- ^ Yu, Y. G., Turner, G. E. and Weiss, R. L. (1996) Acetylglutamate synthase from Neurospora crassa: structure and regulation of expression. Mol. Microbiol. 22, 545–554
- ^ Caldovic, L., Mew, N. A., Shi, D., Morizono, H., Yudkoff, M., and Tuchmana, M. (2010) N-acetylglutamate synthase: structure, function and defects. Mol. Genet. Metab. 100(Suppl 1): S13–S19
- ^ J Cybis, R.H Davis, Organization and control in the arginine biosynthetic pathway of Neurospora. J. Bacteriol., 123 (1975), pp. 196–202
- ^ T Sonoda, M Tatibana, Purification of N-acetyl-l-glutamate synthetase from rat liver mitochondria and substrate and activator specificity of the enzyme. J. Biol. Chem., 258 (1983), pp. 9839–9844
- ^ Meijer, A. J. and Verhoeven, A. J. (1984) N-Acetylglutamate and urea synthesis. Biochem. J. 223, 559–560
- ^ Lund, P. and Wiggins, D. (1984) Is N-acetylglutamate a short-term regulator of urea synthesis? Biochem. J. 218, 991–994
- ^ Caldovic, L., Tuchman, M., N-Acetylglutamate and its changing role through evolution. Biochem. J. (2003) 372 (279–290) doi:10.1042/BJ20030002
- ^ Wong L. J., Wong S. S. Kinetic mechanism of the reaction catalyzed by nuclear histone acetyltransferase from calf thymus. Biochemistry. 1983 Sep 27;22(20):4637-41.
- ^ Dyda, F., Klein, D. C., and Hickman, A. B. (2000) Annu. Rev. Biophys. Biomol. Struct. 29, 81-103
- ^ Shi, D., Sagar, V., Jin, Z., Yu, X., Caldovic, L., Morizono, H., Allewell, N. M. and Tuchman, M. (2008) The Crystal Structure of N-Acetyl-L-glutamate Synthase from Neisseria gonorrhoeae Provides Insights into Mechanisms of Catalysis and Regulation. J. Bio. Chem. 283, 7176-7184
- ^ Min, L., Jin, Z., Caldovic, L., Morizono, H., Allewell, N. M., Tuchman, M. and Shi, D. Mechanism of Allosteric Inhibition of N-Acetyl-L-glutamate Synthase by L-Arginine. J. Bio. Chem. 284, 4873-4880
- ^ a b Morizono, H., Caldovic, L., Shi, D. and Tuchman, M. Mammalian N-Acetylglutamate synthase. Mol Genet Metab. 2004 April; 81(Suppl 1): S4–11.
- ^ a b Caldovic, L., Morizono, H., Panglao, M. G., Cheng, S. F., Packman, S. and Tuchman, M. Null mutations in the N-acetylglutamate synthase gene associated with acute neonatal disease and hyperammonemia. Hum. Gen. April 2003, Vol. 112, 4, pp 364-368
- ^ McCudden, C. R. and Powers-Lee, S. G. Required Allosteric Effector Site for N-Acetylglutamate on Carbamoyl-Phosphate Synthetase I. J. Bio. Chem. 271, 18285-18294
- ^ Caldovic, L., Morizono, H., Daikhin, Y., Nissim, I., McCarter, R. J., Yudkoff, M. and Tuchman, M. Restoration of ureagenesis in N-acetylglutamate synthase deficiency by N-carbamylglutamate. J. Ped. Vol. 145, 4, October 2004, 552–554
- Lehninger principles of biochemistry, 4th edition, David L. Nelson, Michael M. Cox
External links
- GeneReviews/NCBI/NIH/UW entry on Urea Cycle Disorders Overview
- N-Acetylglutamate Synthase at the US National Library of Medicine Medical Subject Headings (MeSH)
|
Transferases: acyltransferases (EC 2.3)
|
|
| 2.3.1: other than amino-acyl groups |
- acetyltransferases: Acetyl-Coenzyme A acetyltransferase
- N-Acetylglutamate synthase
- Choline acetyltransferase
- Dihydrolipoyl transacetylase
- Acetyl-CoA C-acyltransferase
- Beta-galactoside transacetylase
- Chloramphenicol acetyltransferase
- N-acetyltransferase
- Serotonin N-acetyl transferase
- HGSNAT
- ARD1A
- Histone acetyltransferase
- palmitoyltransferases: Carnitine O-palmitoyltransferase
- Serine C-palmitoyltransferase
- other: Acyltransferase like 2
- Aminolevulinic acid synthase
- Beta-ketoacyl-ACP synthase
- Glyceronephosphate O-acyltransferase
- Lecithin—cholesterol acyltransferase
- Glycerol-3-phosphate O-acyltransferase
- 1-acylglycerol-3-phosphate O-acyltransferase
- 2-acylglycerol-3-phosphate O-acyltransferase
- ABHD5
|
|
| 2.3.2: Aminoacyltransferases |
- Gamma-glutamyl transpeptidase
- Peptidyl transferase
- Transglutaminase
- Tissue transglutaminase
- Keratinocyte transglutaminase
- Factor XIII
|
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| 2.3.3: converted into alkyl on transfer |
- Citrate synthase
- ATP citrate lyase
- HMG-CoA synthase
- Malate synthase
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- Biochemistry overview
- Enzymes overview
- By EC number: 1.1
- 2
- 3
- 4
- 5
- 6
- 7
- 8
- 10
- 11
- 13
- 14
- 15-18
- 2.1
- 3.1
- 4.1
- 5.1
- 6.1-3
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Metabolism: amino acid metabolism - urea cycle enzymes
|
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| Main cycle |
|
mitochondrial matrix:
|
- Carbamoyl phosphate synthetase I
- Ornithine transcarbamylase
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cytosol:
|
- Argininosuccinate synthetase
- Argininosuccinate lyase
- Arginase
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|
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| Regulatory/transport |
- N-Acetylglutamate synthase
- Ornithine translocase
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Index of inborn errors of metabolism
|
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| Description |
- Metabolism
- Enzymes and pathways: citric acid cycle
- pentose phosphate
- glycoproteins
- glycosaminoglycans
- phospholipid
- cholesterol and steroid
- sphingolipids
- eicosanoids
- amino acid
- urea cycle
- nucleotide
|
|
| Disorders |
- Citric acid cycle and electron transport chain
- Glycoprotein
- Proteoglycan
- Fatty-acid
- Phospholipid
- Cholesterol and steroid
- Eicosanoid
- Amino acid
- Purine-pyrimidine
- Heme metabolism
- Symptoms and signs
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| Treatment |
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Mitochondrial proteins
|
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| Outer membrane |
| fatty acid degradation |
- Carnitine palmitoyltransferase I
- Long-chain-fatty-acid—CoA ligase
|
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| tryptophan metabolism |
|
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monoamine neurotransmitter
metabolism |
|
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| Intermembrane space |
- Adenylate kinase
- Creatine kinase
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| Inner membrane |
| oxidative phosphorylation |
- Coenzyme Q – cytochrome c reductase
- Cytochrome c
- NADH dehydrogenase
- Succinate dehydrogenase
|
|
| pyrimidine metabolism |
- Dihydroorotate dehydrogenase
|
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| mitochondrial shuttle |
- Malate-aspartate shuttle
- Glycerol phosphate shuttle
|
|
| other |
- Glutamate aspartate transporter
- Glycerol-3-phosphate dehydrogenase
- ATP synthase
- Carnitine palmitoyltransferase II
- Uncoupling protein
|
|
|
| Matrix |
| citric acid cycle |
- Citrate synthase
- Aconitase
- Isocitrate dehydrogenase
- Oxoglutarate dehydrogenase complex
- Succinyl coenzyme A synthetase
- Fumarase
- Malate dehydrogenase
|
|
| anaplerotic reactions |
- Aspartate transaminase
- Glutamate dehydrogenase
- Pyruvate dehydrogenase complex
|
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| urea cycle |
- Carbamoyl phosphate synthetase I
- Ornithine transcarbamylase
- N-Acetylglutamate synthase
|
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| alcohol metabolism |
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| Other/to be sorted |
| steroidogenesis |
- Cholesterol side-chain cleavage enzyme
- Steroid 11-beta-hydroxylase
- Aldosterone synthase
- Frataxin
|
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- Mitochondrial membrane transport protein
- Mitochondrial permeability transition pore
- Mitochondrial carrier
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|
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| Mitochondrial DNA |
| Complex I |
- MT-ND1
- MT-ND2
- MT-ND3
- MT-ND4
- MT-ND4L
- MT-ND5
- MT-ND6
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| Complex III |
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| Complex IV |
|
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| ATP synthase |
|
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| tRNA |
- MT-TA
- MT-TC
- MT-TD
- MT-TE
- MT-TF
- MT-TG
- MT-TH
- MT-TI
- MT-TK
- MT-TL1
- MT-TL2
- MT-TM
- MT-TN
- MT-TP
- MT-TQ
- MT-TR
- MT-TS1
- MT-TS2
- MT-TT
- MT-TV
- MT-TW
- MT-TY
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see also mitochondrial diseases
|
Index of cells
|
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| Description |
- Structure
- Organelles
- peroxisome
- cytoskeleton
- centrosome
- epithelia
- cilia
- mitochondria
- Membranes
- Membrane transport
- ion channels
- vesicular transport
- solute carrier
- ABC transporters
- ATPase
- oxidoreduction-driven
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| Disease |
- Structural
- peroxisome
- cytoskeleton
- cilia
- mitochondria
- nucleus
- scleroprotein
- Membrane
- channelopathy
- solute carrier
- ATPase
- ABC transporters
- other
- extracellular ligands
- cell surface receptors
- intracellular signalling
- Vesicular transport
- Pore-forming toxins
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