English Journal

Biosynthesis of D-xylulose 5-phosphate from D-xylose and polyphosphate through a minimized two-enzyme cascade.

Kim JE1, Zhang YP2,3,4,5.
Biotechnology and bioengineering.Biotechnol Bioeng.2015 Aug 4. doi: 10.1002/bit.25718. [Epub ahead of print]
Sugar phosphates cannot be produced easily by microbial fermentation because negatively-charged compounds cannot be secreted across intact cell membrane. D-xylulose 5-phosphate (Xu5P), a very expensive sugar phosphate, was synthesized from D-xylose and polyphosphate catalyzed by enzyme cascades in o
PMID 26241217

High-yield production of dihydrogen from xylose by using a synthetic enzyme cascade in a cell-free system.

Martín del Campo JS1, Rollin J, Myung S, Chun Y, Chandrayan S, Patiño R, Adams MW, Zhang YH.
Angewandte Chemie (International ed. in English).Angew Chem Int Ed Engl.2013 Apr 22;52(17):4587-90. doi: 10.1002/anie.201300766. Epub 2013 Mar 19.
Let enzymes work: H2 was produced from xylose and water in one reactor containing 13 enzymes (red). By using a novel polyphosphate xylulokinase (XK), xylose was converted into H2 and CO2 with approaching 100 % of the theoretical yield. The findings suggest that cell-free biosystems could produce H
PMID 23512726

Structure and function of human xylulokinase, an enzyme with important roles in carbohydrate metabolism.

Bunker RD1, Bulloch EM, Dickson JM, Loomes KM, Baker EN.
The Journal of biological chemistry.J Biol Chem.2013 Jan 18;288(3):1643-52. doi: 10.1074/jbc.M112.427997. Epub 2012 Nov 23.
D-Xylulokinase (XK; EC 2.7.1.17) catalyzes the ATP-dependent phosphorylation of d-xylulose (Xu) to produce xylulose 5-phosphate (Xu5P). In mammals, XK is the last enzyme in the glucuronate-xylulose pathway, active in the liver and kidneys, and is linked through its product Xu5P to the pentose-phosph
PMID 23179721