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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2012/10/29 16:38:05」(JST)

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RNA polymerase II holoenzyme is a form of eukaryotic RNA polymerase II that is recruited to the promoters of protein-coding genes in living cells. It consists of RNA polymerase II, a subset of general transcription factors, and regulatory proteins known as SRB proteins. Transcription factor II D (TF_IID) is one of several general transcription factors that make up the RNA polymerase II preinitiation complex.^[1] Before the start of transcription, the transcription Factor II D (TFIID) complex, binds to the TATA box in the core promoter of the gene.

Functions

Coordinates the activities of more than 70 polypeptides required for initiation of transcription by RNA polymerase II
Binds to the core promoter to position the polymerase properly
Serves as the scaffold for assembly of the remainder of the transcription complex
Acts as a channel for regulatory signals

Structure

TF_IID is itself composed of several subunits called TBP-associated factors (TAFs, of which there are 16) and the TATA Binding Protein (TBP). In a test tube, only TBP is necessary for transcription at promoters that contain a TATA box.^[2] TAFs, however, add promoter selectivity, especially if there is no TATA box sequence for TBP to bind to. TAFs are included in two distinct complexes, TFIID and B-TFIID.^[3] The TFIID complex is composed of TBP and more than eight TAFs. But, the majority of TBP is present in the B-TFIID complex, which is composed of TBP and TAFII170 (BTAF1) in a 1:1 ratio.^[4]

Subunits in the TFIID complex include:^[2]

TBP (TATA binding protein)
TAF1 (TAFII250)
TAF2 (CIF150)
TAF3 (TAFII140)
TAF4 (TAFII130/135)
TAF4B (TAFII105)
TAF5 (TAFII100)
TAF6 (TAFII70/80)
TAF7 (TAFII55)
TAF8 (TAFII43)
TAF9 (TAFII31/32)
TAF9B (TAFII31L)
TAF10 (TAFII30)
TAF11 (TAFII28)
TAF12 (TAFII20/15)
TAF13 (TAFII18)
TAF15 (TAFII68)

References

^ Lewin, Benjamin (2004). Genes VIII. Upper Saddle River, NJ: Pearson Prentice Hall. pp. 636–637. ISBN 0-13-144946-X.
^ ^a ^b Lee TI, Young RA (2000). "Transcription of eukaryotic protein-coding genes". Annu. Rev. Genet. 34: 77–137. doi:10.1146/annurev.genet.34.1.77. PMID 11092823.
^ "NCBI Entrez GeneID: 9044 BTAF1 BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1 homolog, S. cerevisiae)". http://www.ncbi.nlm.nih.gov/sites/entrez.
^ Timmers HTM, Meyers RE, Sharp PA (Sep 1992). "Composition of transcription factor B-TFIID". Proc Natl Acad Sci USA. 89 (17): 8140–4. doi:10.1073/pnas.89.17.8140. PMC 49872. PMID 1387711. http://www.pnas.org/content/89/17/8140.full.pdf+html.

External links

Transcription+Factor+TFIID at the US National Library of Medicine Medical Subject Headings (MeSH)

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English Journal

SAYP interacts with DHR3 nuclear receptor and participates in ecdysone-dependent transcription regulation.

Vorobyeva NE, Nikolenko JV, Krasnov AN, Kuzmina JL, Panov VV, Nabirochkina EN, Georgieva SG, Shidlovskii YV.SourceRussian Academy of Sciences; Moscow, Russia.
Cell cycle (Georgetown, Tex.).Cell Cycle.2011 Jun 1;10(11):1821-7. Epub 2011 Jun 1.
The role of metazoan coactivator SAYP in nuclear receptor-driven gene activation in the ecdysone cascade of Drosophila is considered. SAYP interacts with DHR3 nuclear receptor and activates the corresponding genes by recruiting the BTFly (Brahma and TFIID) coactivator supercomplex. The knockdown of
PMID 21519192

Japanese Journal

Temporary expression of the TAF10 gene and its requirement for normal development of Arabidopsis thaliana

TAMADA Yosuke,NAKAMORI Kazuki,NAKATANI Hiromi,MATSUDA Kentaro,HATA Shingo,FURUMOTO Tsuyoshi,IZUI Katsura
Plant and cell physiology 48(1), 134-146, 2006-01-01
… TAF10 is one of the TATA box-binding protein (TBP)-associated factors (TAFs), which constitute a TFIID with a TBP. …
NAID 10018520524