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Direct micropuncture evidence that matrix extracellular phosphoglycoprotein inhibits proximal tubular phosphate reabsorption.

Shirley DG1, Faria NJ, Unwin RJ, Dobbie H.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association.Nephrol Dial Transplant.2010 Oct;25(10):3191-5. doi: 10.1093/ndt/gfq263. Epub 2010 May 14.
BACKGROUND: Matrix extracellular phosphoglycoprotein (MEPE) is a putative phosphatonin that we have shown in previous studies to be phosphaturic in rats. Its site of action in the nephron remains to be confirmed.METHODS: We made micropuncture collections from late proximal convoluted tubules in anae
PMID 20472581

Iothalamate measured by capillary electrophoresis is a suitable alternative to radiolabeled inulin in renal micropuncture.

Capasso G1, Unwin RJ, Pica A, Quagliuolo L, Giovane A.
Kidney international.Kidney Int.2002 Sep;62(3):1068-74.
BACKGROUND: Inulin remains the gold standard for measurements of fluid reabsorption (Jv) and single nephron glomerular filtration rate (SNGFR) in micropuncture experiments. However, the method used to measure cold inulin in nanoliter samples is time-consuming, while the use of radiolabeled inulin is
PMID 12164892

A new protocol for the measurement of picomole quantities of magnesium in rat renal tubular fluid.

Kibble JD1, Audsley N, Day JP, Green R.
Experimental physiology.Exp Physiol.1998 Jan;83(1):11-22.
The analysis of picomolar quantities of magnesium by electrothermal atomic absorption spectrophotometry (EAAS) was studied using a Perkin-Elmer-Zeeman 3030 spectrophotometer. The absorbance signal was not heavily dependent on the atomization temperature, but was greatly reduced when ashing temperatu
PMID 9483416

高畠利一,前川正知,太田博真,高田正信,荒井志郎,野村岳而,服部信
日本腎臓学会誌 22(10), 1381-1390, 1980
… As compared to the normal kidney, late proximal ratio of tubular fluid-to-plasma polyfructosan concentration, (TF/P)<SUB>p</SUB>, were lower, with no difference in early distal (TF/P)p in denervated ones. …
NAID 130004063675