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English Journal

Vascular and micro-environmental influences on MSC-coral hydroxyapatite construct-based bone tissue engineering.

Cai L, Wang Q, Gu C, Wu J, Wang J, Kang N, Hu J, Xie F, Yan L, Liu X, Cao Y, Xiao R.SourceResearch Center of Plastic Surgery Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100144, PR China.
Biomaterials.Biomaterials.2011 Nov;32(33):8497-505. Epub 2011 Sep 8.
Bone tissue engineering (BTE) has been demonstrated an effective approach to generate bone tissue and repair bone defect in ectopic and orthotopic sites. The strategy of using a prevascularized tissue-engineered bone grafts (TEBG) fabricated ectopically to repair bone defects, which is called live b
PMID 21855129

Osteogenesis and angiogenesis of tissue-engineered bone constructed by prevascularized β-tricalcium phosphate scaffold and mesenchymal stem cells.

Wang L, Fan H, Zhang ZY, Lou AJ, Pei GX, Jiang S, Mu TW, Qin JJ, Chen SY, Jin D.SourceInstitute of Orthopaedics and Traumatology, The Third Affiliated Hospital, Guangzhou Medical University, People's Republic of China. wangle8273@163.com
Biomaterials.Biomaterials.2010 Dec;31(36):9452-61. Epub 2010 Sep 24.
Although vascularized tissue-engineered bone grafts (TEBG) have been generated ectopically in several studies, the use of prevascularized TEBG for segmental bone defect repair are rarely reported. In current study, we investigated the efficacy of prevascularized TEBG for segmental defect repair. The
PMID 20869769

中原満
泌尿器科紀要 32(11), 1677-1688, 1986-11
… (TA)でも抑制されなかった.2) Scatchard法による検討では最終精製分画の解離定数は2.3 nMと高親和性の結合能を有し,ARの結合特異性を残していた.3)精製倍率は蛋白量あたりの結合能で計算すると約38倍であった.4) Polyacrylamide gel electrophoresis (PAGE)による検討では,albuminやtestosterone-estradiol binding globulin (TeBG)の混入を認めなかったThe androgen receptor (AR) was purified to establish a new method for measuring AR after producing the antibody for the purified …
NAID 120002159406

中原満
泌尿器科紀要 32(11), 1701-1711, 1986-11
… ず,ARとしての高い結合特異性を示した.精製倍率を蛋白量あたりの結合部位数で計算すると,cytosolの約2,360倍となった.7.5%polyacrylamide gel electrophoresisによる検討では,精製AR中にalbumin, testosterone-estradiol binding globulin (TeBG)などの混入を認めなかった.Sephacryl S-300 column chromatographyではvoid volumeに一致した3H-R1881のピークが得られた.これに対して高速液体クロマトグラフィーによる検討では分子量は約32,000と考えられた.両実験におけ …
NAID 120002159404