セファデックス
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出典(authority):フリー百科事典『ウィキペディア(Wikipedia)』「2016/08/30 01:41:26」(JST)
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Sephadex is a trademark for cross-linked dextran gel used for gel filtration. It was launched by Pharmacia in 1959, after development work by Jerker Porath and Per Flodin.[1][2] The name is derived from separation Pharmacia dextran. It is normally manufactured in a bead form and most commonly used for gel filtration columns. By varying the degree of cross-linking, the fractionation properties of the gel can be altered.
These highly specialized gel filtration and chromatographic media are composed of macroscopic beads synthetically derived from the polysaccharide, dextran. The organic chains are cross-linked to give a three-dimensional network having functional ionic groups attached by ether linkages to glucose units of the polysaccharide chains.
Available forms include anion and cation exchangers, as well as gel filtration resins, with varying degrees of porosity; bead sizes fall in discrete ranges between 20 and 300 µm.
Contents
- 1 Applications
- 2 Fractionation
- 3 See also
- 4 References
Applications
Sephadex is used to separate low and high molecular weight molecules. Sephadex is a faster alternative to dialysis (de-salting), requiring a low dilution factor (as little as 1.4:1), with high activity recoveries. Sephadex is also used for buffer exchange and the removal of small molecules during the preparation of large biomolecules, such as ampholytes, detergents, radioactive or fluorescent labels, and phenol (during DNA purification).
Fractionation
Fractionation Range[citation needed] of Globular Proteins (Da)
Gel Type |
Fractionation Range |
G-10 |
≤700 |
G-15 |
≤1500 |
G-25 |
1000–5000 |
G-50 |
1500–30,000 |
G-75 |
3000–80,000 |
G-100 |
4000–150,000 |
G-150 |
5000–300,000 |
G-200 |
5000–600,000 |
See also
- PEGylation
- Size exclusion chromatography
References
- ^ chemeurope.com: From Sephadex to GE Healthcare, accessed on June 17, 2009
- ^ Gel filtration: a method for desalting and group separation, Nature, vol. 183 (1959), s. 1657-1659
UpToDate Contents
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- Cui C, Zhang S, You L, Ren J, Luo W, Chen W, Zhao M.SourceCollege of Light Industry and Food, South China University of Technology, Guangzhou 510640, China.
- Food chemistry.Food Chem.2013 Aug 15;139(1-4):1-8. doi: 10.1016/j.foodchem.2013.01.107. Epub 2013 Feb 10.
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- PNIPAAm-grafted thermoresponsive microcarriers: Surface-initiated ATRP synthesis and characterization.
- Cakmak S, Cakmak AS, Gümüşderelioğlu M.SourceNanotechnology and Nanomedicine Department, Hacettepe University, 06800, Beytepe, Ankara, Turkey.
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- Mild acid degradation of the lipopolysaccharide of Edwardsiella tarda PCM 1156 afforded an O-polysaccharide, which was isolated by gel-permeation chromatography on Sephadex G-50 and studied by sugar and methylation analyses along with (1)H NMR and (13)C NMR spectroscopy, including 2D (1)H,(1)H COSY,
- PMID 23623959
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- NAID 130005071486
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- … Tilapia NAGase was purified to be PAGE homogeneous by the following techniques: (NH_4)_2SO_4 fractionation (40-55%), DEAE-cellulose (DE-32) ion exchange chromatography, Sephadex G-200 gel filtration and DEAE-Sephadex (A-50). …
- NAID 110009804065
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