SARS-CoV

出典: meddic

SARSコロナウイルス

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出典(authority):フリー百科事典『ウィキペディア(Wikipedia)』「2013/08/12 16:35:09」(JST)

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英文文献

  • Yeast-based assays for the high-throughput screening of inhibitors of coronavirus RNA cap guanine-N7-methyltransferase.
  • Sun Y1, Wang Z1, Tao J1, Wang Y1, Wu A1, Yang Z2, Wang K2, Shi L2, Chen Y3, Guo D4.Author information 1State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, PR China.2Biological Pesticide Engineering Research Center, Hubei Academy of Agricultural Science, Hubei 430072, PR China.3State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, PR China. Electronic address: chenyu@whu.edu.cn.4State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, PR China. Electronic address: dguo@whu.edu.cn.AbstractThe 5'-cap structure is a distinct feature of eukaryotic mRNAs and is important for RNA stability and protein translation by providing a molecular signature for the distinction of self or non-self mRNA. Eukaryotic viruses generally modify the 5'-end of their RNAs to mimic the cellular mRNA structure, thereby facilitating viral replication in host cells. However, the molecular organization and biochemical mechanisms of the viral capping apparatus typically differ from its cellular counterpart, which makes viral capping enzymes attractive targets for drug discovery. Our previous work showed that SARS coronavirus (SARS-CoV) non-structural protein 14 represents a structurally novel and unique guanine-N7-methyltransferase (N7-MTase) that is able to functionally complement yeast cellular N7-MTase. In the present study, we developed a yeast-based system for identifying and screening inhibitors against coronavirus N7-MTase using both 96-well and 384-well microtiter plates. The MTase inhibitors previously identified by in vitro biochemical assays were tested, and some, such as sinefungin, effectively suppressed N7-MTase in the yeast system. However, other compounds, such as ATA and AdoHcy, did not exert an inhibitory effect within a cellular context. These results validated the yeast assay system for inhibitor screening yet also demonstrated the difference between cell-based and in vitro biochemical assays. The yeast system was applied to the screening of 3000 natural product extracts, and three were observed to more potently inhibit the activity of coronavirus than human N7-MTase.
  • Antiviral research.Antiviral Res.2014 Apr;104:156-64. doi: 10.1016/j.antiviral.2014.02.002. Epub 2014 Feb 11.
  • The 5'-cap structure is a distinct feature of eukaryotic mRNAs and is important for RNA stability and protein translation by providing a molecular signature for the distinction of self or non-self mRNA. Eukaryotic viruses generally modify the 5'-end of their RNAs to mimic the cellular mRNA structure
  • PMID 24530452
  • The ORF4a protein of human coronavirus 229E functions as a viroporin that regulates viral production.
  • Zhang R1, Wang K2, Lv W2, Yu W2, Xie S2, Xu K2, Schwarz W3, Xiong S4, Sun B5.Author information 1Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, China; Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025, China.2Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025, China.3Goethe-University Frankfurt, Institute for Biophysics, Max-von-Laue-Str. 1, D-60438 Frankfurt am Main, Germany; Shanghai Research Center for Acupuncture and Meridian, 199 Guoshoujing Road, Shanghai 201023, China.4Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, China. Electronic address: sdxiongfd@126.com.5Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025, China; State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China. Electronic address: bsun@sibs.ac.cn.AbstractIn addition to a set of canonical genes, coronaviruses encode additional accessory proteins. A locus located between the spike and envelope genes is conserved in all coronaviruses and contains a complete or truncated open reading frame (ORF). Previously, we demonstrated that this locus, which contains the gene for accessory protein 3a from severe acute respiratory syndrome coronavirus (SARS-CoV), encodes a protein that forms ion channels and regulates virus release. In the current study, we explored whether the ORF4a protein of HCoV-229E has similar functions. Our findings revealed that the ORF4a proteins were expressed in infected cells and localized at the endoplasmic reticulum/Golgi intermediate compartment (ERGIC). The ORF4a proteins formed homo-oligomers through disulfide bridges and possessed ion channel activity in both Xenopus oocytes and yeast. Based on the measurement of conductance to different monovalent cations, the ORF4a was suggested to form a non-selective channel for monovalent cations, although Li(+) partially reduced the inward current. Furthermore, viral production decreased when the ORF4a protein expression was suppressed by siRNA in infected cells. Collectively, this evidence indicates that the HCoV-229E ORF4a protein is functionally analogous to the SARS-CoV 3a protein, which also acts as a viroporin that regulates virus production. This article is part of a Special Issue entitled: Viral Membrane Proteins - Channels for Cellular Networking.
  • Biochimica et biophysica acta.Biochim Biophys Acta.2014 Apr;1838(4):1088-95. doi: 10.1016/j.bbamem.2013.07.025. Epub 2013 Jul 29.
  • In addition to a set of canonical genes, coronaviruses encode additional accessory proteins. A locus located between the spike and envelope genes is conserved in all coronaviruses and contains a complete or truncated open reading frame (ORF). Previously, we demonstrated that this locus, which contai
  • PMID 23906728
  • SARS coronavirus papain-like protease inhibits the type I interferon signaling pathway through interaction with the STING-TRAF3-TBK1 complex.
  • Chen X1, Yang X, Zheng Y, Yang Y, Xing Y, Chen Z.Author information 1Division of Infection and Immunity, Department of Electromagnetic and Laser Biology, Beijing Institute of Radiation Medicine, Beijing, 100850, China.AbstractSARS coronavirus (SARS-CoV) develops an antagonistic mechanism by which to evade the antiviral activities of interferon (IFN). Previous studies suggested that SARS-CoV papain-like protease (PLpro) inhibits activation of the IRF3 pathway, which would normally elicit a robust IFN response, but the mechanism(s) used by SARS PLpro to inhibit activation of the IRF3 pathway is not fully known. In this study, we uncovered a novel mechanism that may explain how SARS PLpro efficiently inhibits activation of the IRF3 pathway. We found that expression of the membrane-anchored PLpro domain (PLpro-TM) from SARS-CoV inhibits STING/TBK1/IKKε-mediated activation of type I IFNs and disrupts the phosphorylation and dimerization of IRF3, which are activated by STING and TBK1. Meanwhile, we showed that PLpro-TM physically interacts with TRAF3, TBK1, IKKε, STING, and IRF3, the key components that assemble the STING-TRAF3-TBK1 complex for activation of IFN expression. However, the interaction between the components in STING-TRAF3-TBK1 complex is disrupted by PLpro-TM. Furthermore, SARS PLpro-TM reduces the levels of ubiquitinated forms of RIG-I, STING, TRAF3, TBK1, and IRF3 in the STING-TRAF3-TBK1 complex. These results collectively point to a new mechanism used by SARS-CoV through which PLpro negatively regulates IRF3 activation by interaction with STING-TRAF3-TBK1 complex, yielding a SARS-CoV countermeasure against host innate immunity.
  • Protein & cell.Protein Cell.2014 Mar 14. [Epub ahead of print]
  • SARS coronavirus (SARS-CoV) develops an antagonistic mechanism by which to evade the antiviral activities of interferon (IFN). Previous studies suggested that SARS-CoV papain-like protease (PLpro) inhibits activation of the IRF3 pathway, which would normally elicit a robust IFN response, but the mec
  • PMID 24622840

和文文献

  • Synthesis of Bioactive Natural Products as Protein Inhibitors
  • KONNO Hiroyuki
  • Bioscience, biotechnology, and biochemistry 76(7), 1257-1261, 2012-07-23
  • NAID 10030981537
  • Design, Synthesis and Evalution of Tripeptidomimetics with Arylketone as Inhibitors of SARS-CoV 3CL Protease
  • KONNO Sho,NAKADA Kiyohiko,THANIGAIMALAI Pillaiyar,KAKIUCHI Rie,YAMAMOTO Takehito,YAMAZAKI Yuri,YAKUSHIJI Fumika,AKAJI Kenichi,KISO Yoshiaki,KAWASAKI Yuko,FRIERE Ernest,HAYASHI Yoshio
  • Peptide science : proceedings of the ... Japanese Peptide Symposium 2011, 151-154, 2012-03-01
  • NAID 10030204179
  • Diarylheptanoids from Alnus japonica Inhibit Papain-Like Protease of Severe Acute Respiratory Syndrome Coronavirus
  • Park Ji-Young,Jae Jeong Hyung,Hoon Kim Jang,Min Kim Young,Park Su-Jin,Kim Doman,Hun Park Ki,Song Lee Woo,Bae Ryu Young
  • Biological and Pharmaceutical Bulletin 35(11), 2036-2042, 2012
  • … The papain-like protease (PLpro), which controls replication of the severe acute respiratory syndrome coronavirus (SARS-CoV), has been identified as a potential drug target for the treatment of SARS. … An intensive hunt for effective anti-SARS drugs has been undertaken by screening for natural product inhibitors that target SARS-CoV PLpro. …
  • NAID 130001872274

関連リンク

SARSコロナウイルス(サーズコロナウイルス)(英: SARS coronavirus,SARS-CoV)は 、重症急性呼吸器症候群 (Severe Acute Respiratory Syndrome, SARS) の病原体 として同定された新種のコロナウイルスである。通称SARSウイルス。飛沫感染により ...
The SARS coronavirus, sometimes shortened to SARS-CoV, is the virus that causes severe acute respiratory syndrome (SARS). In April 16 of 2003, following the outbreak of SARS in Asia and secondary cases elsewhere in the world, the ...

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★リンクテーブル★
先読みSARSコロナウイルス
リンク元重症急性呼吸器症候群」「SARS coronavirus」「SARS virus」「SARS-associated coronavirus
関連記事C」「SA」「Co」「SARS」「SAR

SARSコロナウイルス」

  [★]

SARS coronavirusSARS virusSARS-associated coronavirussevere acute respiratory syndrome-associated coronaviarus
コロナウイルス, SARS

ウイルス学

感染症

  • severe acute respiratory sundrome SARS

疫学

  • 2002年11月中国で始まり2003年に世界に広がった。

感染経路

  • 飛沫感染(くしゃみ・咳)
  • 接触感染



重症急性呼吸器症候群」

  [★]

severe acute respiratory syndrome SARS

歴史

  • 2002年11月から2003年2月まで中国広東省に多数の感染者が発生し、香港、ハノイ、カナダ、シンガポール台湾で感染が拡大

病原体

疫学

感染経路

  • 飛沫感染  空気感染ではない
  • 病初期にウイルスの排出量は少ない

症状

  • 38℃以上の発熱、悪寒、咳、呼吸困難、頭痛、下痢、全身倦怠感など



SARS coronavirus」

  [★]

SARS virusSARS-associated coronavirusSARS-CoV


SARS virus」

  [★]

SARSウイルス

SARS coronavirusSARS-associated coronavirusSARS-CoV


SARS-associated coronavirus」

  [★]

SARS coronavirusSARS virusSARS-CoV


C」

  [★]

WordNet   license wordnet

「the 3rd letter of the Roman alphabet」
c

WordNet   license wordnet

「(music) the keynote of the scale of C major」

WordNet   license wordnet

「a general-purpose programing language closely associated with the UNIX operating system」

PrepTutorEJDIC   license prepejdic

「carbonの化学記号」


SA」

  [★]

WordNet   license wordnet

「Nazi militia created by Hitler in 1921 that helped him to power but was eclipsed by the SS after 1943」
Sturmabteilung, Storm Troops


Co」

  [★] コバルト cobalt

PrepTutorEJDIC   license prepejdic

「cobaltの化学記号」


SARS」

  [★] 重症急性呼吸器症候群 severe acute respiratory syndrome


SAR」

  [★]


seasonal allergic rhinitis




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