関: ribonuclease protection assay

WordNet

a quantitative or qualitative test of a substance (especially an ore or a drug) to determine its components; frequently used to test for the presence or concentration of infectious agents or antibodies etc.
analyze (chemical substances)
a substance that is undergoing an analysis of its components
an appraisal of the state of affairs; "they made an assay of the contents"; "a check on its dependability under stress" (同)check
a written report of the results of an analysis of the composition of some substance
the condition of being protected; "they were huddled together for protection"; "he enjoyed a sense of peace and protection in his new home" (同)shelter
the imposition of duties or quotas on imports in order to protect domestic industry against foreign competition; "he made trade protection a plank in the party platform" (同)trade protection
payment extorted by gangsters on threat of violence; "every store in the neighborhood had to pay him protection" (同)tribute
the activity of protecting someone or something; "the witnesses demanded police protection"
hardy and sure-footed animal smaller and with longer ears than the horse
a pompous fool

PrepTutorEJDIC

(金属の品質・鉱石の金属含有量・薬品の)分析,試金 / 試金物,分析物 / …'を'試金する,分析する
〈U〉(…から)『保護すること』,『守ること』;保護されていること《+from(against)+名》 / 《単数形で》(…を)防いでくれる人(物),保護者《+『from』(『against』)+『名』》
ロバ / ばか者
《米俗》しり,けつ(《英俗》arse)

Wikipedia preview

出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2013/04/16 19:13:20」(JST)

wiki en

Nuclease protection assay is a laboratory technique used in biochemistry and genetics to identify individual RNA molecules in a heterogeneous RNA sample extracted from cells. The technique can identify one or more RNA molecules of known sequence even at low total concentration. The extracted RNA is first mixed with antisense RNA or DNA probes that are complementary to the sequence or sequences of interest and the complementary strands are hybridized to form double-stranded RNA (or a DNA-RNA hybrid). The mixture is then exposed to ribonucleases that specifically cleave only single-stranded RNA but have no activity against double-stranded RNA. When the reaction runs to completion, susceptible RNA regions are degraded to very short oligomers or to individual nucleotides; the surviving RNA fragments are those that were complementary to the added antisense strand and thus contained the sequence of interest.

Probe

The probes are prepared by cloning part of the gene of interest in a vector under the control of any of the following promoters, SP6, T7 or T3. These promoters are recognized by DNA dependent RNA polymerases originally characterized from bacteriophages. The probes produced are radioactive as they are prepared by in vitro transcription using radioactive UTPs. Uncomplemented DNA or RNA is cleaved off by nucleases. When the probe is a DNA molecule, S1 nuclease is used; when the probe is RNA, any single-strand-specific ribonuclease can be used. Thus the surviving probe-mRNA complement is simply detected by autoradiography.

Uses

Nuclease protection assays are used to map introns and 5' and 3' ends of transcribed gene regions. Quantitative results can be obtained regarding the amount of the target RNA present in the original cellular extract - if the target is a messenger RNA, this can indicate the level of transcription of the gene in the cell.

They are also used to detect the presence of double stranded RNA, presence of which could mean RNA interference.

Northern blotting is a laboratory technique that produces similar information. It is slower and less quantitative, but also produces accurate information about the size of the target RNA. Nuclease protection assay products are limited to the size of the initial probes due to the destruction of the non-hybridized RNA during the nuclease digestion step.

References

Sandelin, A. et al. Mammalian RNA polymerase II core promoters: insights from genome-wide studies. Nature Rev. Genet. 8, 424–436 (2007)

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English Journal

Detection of human norovirus in cherry tomatoes, blueberries and vegetable salad by using a receptor-binding capture and magnetic sequestration (RBCMS) method.

Pan L, Zhang Q, Li X, Tian P.SourceShanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200135, China. panlw888@126.com
Food microbiology.Food Microbiol.2012 Jun;30(2):420-6. Epub 2012 Jan 11.
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PMID 22365355

Aclarubicin enhances tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis through death receptor 5 upregulation.

Horinaka M, Yoshida T, Nakata S, Shiraishi T, Tomosugi M, Yoshikawa S, Wakada M, Sakai T.SourceDepartment of Molecular-Targeting Cancer Prevention, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan.
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Japanese Journal

Lipopolysaccharide Induces Atrial Arrhythmogenesis via Down-Regulation of L-Type Ca2+ Channel Genes in Rats

Okazaki Reiko,Iwasaki Yu-ki,Miyauchi Yasushi,Hirayama Yoshiyuki,Kobayashi Yoshinori,Katoh Takao,Mizuno Kyoichi,Sekiguchi Akiko,Yamashita Takeshi
International Heart Journal 50(3), 353-363, 2009
… Hemodynamic data were obtained and the atrial appendages were removed after LPS injection (0, 3, 6, 12, and 24 hours) for an RNase protection assay for α1C, β2, α1G, and SCN5A. …
NAID 130000120820

Gene expression of PROSC (proline synthetase co-transcribed) in related to cytosine arabinoside sensitivity in human leukemia

FUJIMAKI Shin-ich,TAKEDA Mayu,SAITO Kuniaki,FUNATO Tadao
Journal of electrophoresis 52(3), 53-56, 2008-09-01
… RNase protection assay and RT-PCR analysis revealed increased expression of PROSC gene in K562 sensitive cells compared with that of ara-C resistant K562 cells. …
NAID 10024310918

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リンク元	「ribonuclease protection assay」「RNaseプロテクションアッセイ」「リボヌクレアーゼプロテクションアッセイ」
関連記事	「assay」「protection」