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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2015/08/12 08:33:25」(JST)

In biochemistry, PMSF (phenylmethanesulfonylfluoride or phenylmethylsulfonyl fluoride) is a serine protease inhibitor commonly used in the preparation of cell lysates. PMSF does not inhibit all serine proteases^{[citation needed]}. It is rapidly degraded in water and stock solutions are usually made up in anhydrous ethanol, isopropanol, corn oil, or DMSO. Proteolytic inhibition occurs when a concentration between 0.1 - 1 mM PMSF is used. The half-life is short in aqueous solutions (110 min at pH 7, 55 min at pH 7.5, and 35 min at pH 8, all at 25 °C).^[1]

PMSF binds specifically to the active site serine residue in a serine protease. It does not bind to any other serine residues in the protein. This is a result of the hyperactivity of that serine residue caused by the specific environmental conditions in the enzyme's active site. Because PMSF binds covalently to the enzyme, the complex can be viewed by X-ray crystallography; it can therefore be used as a chemical label to identify an essential active site serine in an enzyme.

Enzyme(active)Ser-O-H + F-SO₂CH₂C₆H₅ → EnzymeSer-O-SO₂CH₂C₆H₅ + HF

Serine protease + PMSF → Irreversible enzyme-PMS complex + HF

The LD₅₀ is less than 500 mg/kg. PMSF is a cytotoxic chemical which should be handled only inside a fume hood.

PMSF is commonly used in protein solublization in order to deactivate proteases from digesting proteins of interest after cell lysis.

References

^ GT James (1978). "Inactivation of the protease inhibitor phenylmethylsulfonyl fluoride in buffers". Analytical Biochemistry 86 (2): 574–9. doi:10.1016/0003-2697(78)90784-4. PMID 26289.

External links

The MEROPS online database for peptidases and their inhibitors: PMSF

English Journal

Biochemical characterization of trypsins from the hepatopancreas of Japanese sea bass (Lateolabrax japonicus).

Cai QF, Jiang YK, Zhou LG, Sun LC, Liu GM, Osatomi K, Cao MJ.SourceCollege of Biological Engineering, The Key Laboratory of Science and Technology for Aquaculture and Food Safety, Jimei University, Xiamen, China.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology.Comp Biochem Physiol B Biochem Mol Biol.2011 Jul;159(3):183-9. Epub 2011 Apr 15.
A cationic trypsin (trypsin A) and an anionic trypsin (trypsin B) were highly purified from the hepatopancreas of the Japanese sea bass (Lateolabrax japonicus) by ammonium sulfate precipitation, column chromatographies of DEAE-Sepharose and Sephacryl S-200 HR. Purified trypsins revealed single band
PMID 21515400

A novel nonionic surfactant- and solvent-stable alkaline serine protease from Serratia sp. SYBC H with duckweed as nitrogen source: production, purification, characteristics and application.

Li GY, Cai YJ, Liao XR, Yin J.SourceThe Key Laboratory of Industrial Biotechnology, Department of Education, Ministry of Education, School of Biotechnology, Jiangnan University, Lihu Road 1800, 214122, Wuxi, Jiangsu Province, China.
Journal of industrial microbiology & biotechnology.J Ind Microbiol Biotechnol.2011 Jul;38(7):845-53. Epub 2010 Nov 12.
A novel nonionic surfactant- and hydrophilic solvent-stable alkaline serine protease was purified from the culture supernatant of Serratia sp. SYBC H with duckweed as nitrogen source. The molecular mass of the purified protease is about 59?kDa as assayed via SDS-PAGE. The protease is highly active
PMID 21072556

Japanese Journal

Induction of ovulation in Xenopus without hCG injection: the effect of adding steroids into the aquatic environment.

Ogawa Aoi,Dake Junpei,Iwashina Yu-ki,Tokumoto Toshinobu
Reproductive Biology and Endocrinology 9, 11-1, 2011-01-21
… However, in order to perform such studies, eggs are normally collected after the injection of hCG into the dorsal lymph sac of fully-grown female frogs following pre-injection of PMSF. …
NAID 120002829527

Flavonol Glycosides from Muehlenbeckia platyclada and Their Anti-inflammatory Activity

YEN Chiao-Ting,HSIEH Pei-Wen,HWANG Tsong-Long,LAN Yu-Hsuan,CHANG Fang-Rong,WU Yang-Chang
Chemical & pharmaceutical bulletin 57(3), 280-282, 2009-03-01
… values of 3.82±0.80, 8.61±1.38 and 4.37±0.72μg/ml, respectively, and were 15-fold more potent than phenylmethylsulfonyl fluoride (PMSF), the positive control, in this anti-inflammatory assay. …
NAID 110007122356

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リンク元	「phenylmethylsulfonyl fluoride」「フッ化フェニルメチルスルホニル」
関連記事	「PM」「PMS」

「phenylmethylsulfonyl fluoride」

PMSF
Names
	IUPAC name phenylmethanesulfonyl fluoride
Identifiers
CAS Registry Number	329-98-6
ChEBI	CHEBI:8102
ChEMBL	ChEMBL190503
ChemSpider	4620
	InChI InChI=1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2 ; Key: YBYRMVIVWMBXKQ-UHFFFAOYSA-N ; InChI=1/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2; Key: YBYRMVIVWMBXKQ-UHFFFAOYAF ;
Jmol-3D images	Image
KEGG	C06747
MeSH	Phenylmethylsulfonyl+fluoride
PubChem	4784
	SMILES O=S(F)(=O)Cc1ccccc1 ;
Properties
Chemical formula	C7H7FO2S
Molar mass	174.194
	Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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	Infobox references

フッ化フェニルメチルスルホニル
	IUPAC名 phenylmethanesulfonyl fluoride
識別情報
CAS登録番号	329-98-6
PubChem	4784
ChemSpider	4620
KEGG	C06747
MeSH	Phenylmethylsulfonyl+fluoride
ChEBI	CHEBI:8102
ChEMBL	CHEMBL190503
	SMILES O=S(F)(=O)Cc1ccccc1;
	InChI InChI=1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2 ; Key: YBYRMVIVWMBXKQ-UHFFFAOYSA-N InChI=1/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2; Key: YBYRMVIVWMBXKQ-UHFFFAOYAF;
特性
化学式	C7H7FO2S
モル質量	174.194
	特記なき場合、データは常温 (25 °C)・常圧 (100 kPa) におけるものである。